Molecular basis of cholesterol efflux by ABCG1

• Project/Area Number: 21688007
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Applied biochemistry
• Research Institution: Kyoto University
• Principal Investigator: MATSUO Michinori 京都大学, 大学院・農学研究科, 助教 (00335308)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000); Fiscal Year 2011: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000); Fiscal Year 2010: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000); Fiscal Year 2009: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
• Keywords: ABCタンパク質 / 脂質 / コレステロール / 動脈硬化 / アルツハイマー病 / トランスポーター / リン脂質 / 膜脂質ラフト / アミロイド / 界面活性剤 / 脂質ラフト / 輸送基質

Research Abstract

It was demonstrated that ABCG_1 and ABCG_4 , a lipid transporter, suppress amyloidβsecretion and that ABCG_1 stimulates axonal extension of central nervous neurons. Analysis of purified proteins suggested that ABCG_1 transports cholesterol and choline phospholipid, while ABCG_4 transports cholesterol. It was also demonstrated that ABCG_1 and ABCG_4 distribute to distinct domains on the plasma membrane and transport lipid by modulating the domain structures.

Research Products

• [Journal Article] ATPase activity of human ABCG1 is stimulated by cholesterol and sphingomyelin. (2013)
  • Author(s): Hirayama, H., Kimura, Y., Kioka, N., Matsuo,M. and Ueda, K.
  • Journal Title: J. Lipid Res. Volume: 54 Issue: 2 Pages: 496-502
  • DOI: 10.1194/jlr.m033209
  • Peer Reviewed
• [Journal Article] ATPase activity of nucleotide binding domains of human MDR3 in the context of MDR1 (2013)
  • Author(s): Ishigami, M.. Tominaga, Y., Nagao, K., Kimura, Y., Matsuo, M., Kioka, N. and Ueda, K
  • Journal Title: Biochim Biophys Acta-MCBL Volume: 1831 Issue: 4 Pages: 683-690
  • DOI: 10.1016/j.bbalip.2012.12.016
  • Peer Reviewed
• [Journal Article] ATP hydrolysis-dependent conformational changes in the extracellular domain of ABCA1 are associated with apoA-I binding. (2012)
  • Author(s): Nagao, K., Takahashi, K., Azuma, Y., Takada, M., Kimura, Y., Matsuo, M., Kioka, N. and Ueda, K.
  • Journal Title: J Lipid Res Volume: 53 Issue: 1 Pages: 126-136
  • DOI: 10.1194/jlr.m019976
  • Peer Reviewed
• [Journal Article] Role of Dlg5/1p-dlg, a membrane-associated guanylate kinase family protein, in epithelial-mesenchymal transition in LLc-PK1 renal epithelial cells (2012)
  • Author(s): Takuhito Sezaki, Kohki Inada, Takayuki Sogabe, Kumiyo Kakuda, Lucia Tomiyama, Yohsuke Matsuno, Takafumi Ichikawa, Michinori Matsuo, Kazumitsu Ueda, Noriyuki Kioka
  • Journal Title: PLoS ONE Volume: 7 Issue: 4 Pages: e35519-e35519
  • DOI: 10.1371/journal.pone.0035519
  • Peer Reviewed
• [Journal Article] Involvement of low density lipoprotein receptor-related protein and ABCG1 in stimulation of axonal extension by apo E-containing lipoproteins. (2011)
  • Author(s): Matsuo, M., Campenot, R. B., Vance, D. E., Ueda, K., and Vance J. E
  • Journal Title: Biochim. Biophys. Acta Volume: 1811 Issue: 1 Pages: 31-38
  • DOI: 10.1016/j.bbalip.2010.10.004
  • Peer Reviewed
• [Journal Article] Involvement of low density lipoprotein receptor-related protein and ABCG1 in stimulation of axonal extension by apo E-containing lipoproteins (2011)
  • Author(s): Matsuo, M., Campenot, R.B., Vance, D.E., Ueda, K., Vance J.E.
  • Journal Title: Biochim.Biophys.Acta Volume: 1811 Pages: 31-38
  • Peer Reviewed
• [Journal Article] ATP-Binding Cassette Proteins Involved in Glucose and Lipid Homeostasis (2010)
  • Author(s): Matsuo, M.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 74 Issue: 5 Pages: 899-907
  • DOI: 10.1271/bbb.90921
  • NAID: 10027554433
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Lipid outward translocation by ABC proteins (2010)
  • Author(s): Nagao, K., Kimura, Y., Matsuo, M. and Ueda, K
  • Journal Title: FEBS Lett Volume: 584 Issue: 13 Pages: 2717-2723
  • DOI: 10.1016/j.febslet.2010.04.036
  • Peer Reviewed
• [Journal Article] ABC proteins involved in glucose and lipid homeostasis (2010)
  • Author(s): Matsuo, M.
  • Journal Title: Biosci.Biotechnol.Biochem. Volume: 74 Pages: 899-907
  • Peer Reviewed
• [Journal Article] Lipid outward translocation by ABC proteins (2010)
  • Author(s): Nagao, K., Kimura, Y., Matsuo, M., Ueda, K.
  • Journal Title: FEBS Lett. Volume: 584 Pages: 2717-2723
  • Peer Reviewed
• [Presentation] Phosphorylation by protein kinase C stabilizes ABCG1 (2013)
  • Author(s): Michinori Matsuo, Taro Watanabe, Kazumitsu Ueda
  • Organizer: The Fourteenth International MembraneResearch Forum
  • Place of Presentation: Kyoto
• [Presentation] Phosphorylation by protein kinase C stabilizes ABCG1 (2013)
  • Author(s): Michinori Matsuo, Taro Watanabe, Kazumitsu Ueda
  • Organizer: The Fourteenth International Membrane Research Forum
  • Place of Presentation: Kyoto
• [Presentation] ABC proteins regulate the integrity of membrane meso-domains (2012)
  • Author(s): Michinori Matsuo, Hiromune Ando, Makoto Kiso, and Kazumisu Ueda
  • Organizer: iCeMS retreat
  • Place of Presentation: Osaka
  • Year and Date: 2012-08-31
• [Presentation] ABCA1とABCG1によるコレステロール排出のハイスループット測定系の開発 (2012)
  • Author(s): 松尾道憲、江澤美智、植田和光
  • Organizer: 農芸化学会2011年度大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-23
• [Presentation] ABCG1 is involved in stimulation of axonal extension by apoE-containing lipoproteins (2012)
  • Author(s): Michinori Matsuo, Robert B. Campenot, Dennis E. Vance, Kazumitsu Ueda, and Jean E. Vance
  • Organizer: 4th FEBS special meeting "ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases"
  • Place of Presentation: Innsbruck, Austria
• [Presentation] ABCトランスポーターの膜脂質ラフトへの分布と機能の連関 (2011)
  • Author(s): 松尾道憲、佐野修、加藤玲子、清水裕二、植田和光
  • Organizer: 日光シンポジウム-膜輸送体研究の前衛を目指して
  • Place of Presentation: 日光
  • Year and Date: 2011-12-18
• [Presentation] ABC トランスポーターと疾患:ABCA1 とABCG1 による脂質恒常性維持 ABC transporters and diseases: Lipid homeostasis maintained by ABCA1and ABCG1 (2011)
  • Author(s): 松尾道憲、植田和光
  • Organizer: 日本生化学会2011年度大会シンポジウム「疾患リスクおよび治療バイオマーカーとしてのABCトランスポーターの遺伝子変異と多型」
  • Place of Presentation: 京都
  • Year and Date: 2011-09-23
• [Presentation] 脂質排出ABCトランスポーターの膜ラフトドメインへの局在 (2011)
  • Author(s): 松尾道憲、佐野修、加藤玲子、清水裕二、植田和光
  • Organizer: 第5回トランスポーター研究会九州部会
  • Place of Presentation: 宮崎
  • Year and Date: 2011-09-17
• [Presentation] ABCA1とABCG1とABCG4は異なる膜ドメインに局在し膜脂質ラフと構造を変える (2011)
  • Author(s): 松尾道憲、佐野修、加藤玲子、清水裕二、植田和光
  • Organizer: 第6回トランスポーター研究会年会
  • Place of Presentation: 仙台
  • Year and Date: 2011-06-11
• [Presentation] ABCG1とABCG4がアミロイド前駆体タンパク質のプロセシングに与える影響 (2011)
  • Author(s): 松尾道憲、佐野修、加藤玲子、清水裕二、植田和光
  • Organizer: 日本農芸化学会2011 年度大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-26
• [Presentation] Yuuji Shimizu and Kazumitsu UedaDistribution of ABCG1 and ABCG4 to membrane raft domains (2011)
  • Author(s): Michinori Matsuo, Osamu Sano, Reiko Kato
  • Organizer: ABC 2011 in Kyoto
  • Place of Presentation: Kyoto
• [Presentation] Mechanisms by which apoE-containing lipoproteins stimulate axonal extension of CNS neurons (2011)
  • Author(s): Michinori Matsuo, Robert B. Campenot, Dennis E. Vance, Kazumitsu Ueda, and Jean E. Vance
  • Organizer: ABC 2011 in Kyoto
  • Place of Presentation: Kyoto
• [Presentation] 脂質代謝とABCトランスポーター (2010)
  • Author(s): 松尾道憲、木村泰久、植田和光
  • Organizer: 第130回薬学会130年会記念型シンポジウム トランスポーター:異物解毒と栄養素供給・利用との境界Transporter: Boundary of the Membrane Transports Xenobiotics and Nutrients
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-29
• [Presentation] 脂質代謝とABCトランスポーター (2010)
  • Author(s): 松尾道憲、木村泰久、植田和光
  • Organizer: 第130回薬学会130年会記念型シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-29
• [Presentation] Expression of ABCG1 or ABCG4 modulates raft domains, which suppresses the production of amyloid beta (2010)
  • Author(s): Michinori Matsuo
  • Organizer: 3rd FEBS special meeting "ATP-Binding Cassette (ABC) Proteins
  • Place of Presentation: オーストリア・インスブルク
  • Year and Date: 2010-02-28
• [Presentation] Mechanisms by which glia-derived apoE-containing lipoproteins stimulate axon elongation of CNS neurons (2010)
  • Author(s): Michinori Matsuo
  • Organizer: The thirteenth MEMBRANE RESEARCH FORUM
  • Place of Presentation: 京都
  • Year and Date: 2010-01-28
• [Presentation] アポリポタンパク質E含有リポタンパク質はLRP1を介して中枢神経細胞の軸索伸長を促進する (2010)
  • Author(s): 松尾道憲、Robert B. Campenot、植田和光、Jean E. Vance
  • Organizer: BMB2010
  • Place of Presentation: 神戸
• [Presentation] アポリポタンパク質E含有リポタンパク質が中枢神経細胞の軸索伸長を促進する分子機構 (2010)
  • Author(s): 松尾道憲、Robert B. Campenot、植田和光、Jean E. Vance
  • Organizer: 第5回トランスポーター研究会年会
  • Place of Presentation: 東京医科大学
• [Presentation] Yuuji Shimizu and Kazumitsu UedaExpression of ABCG1 or ABCG4 modulates raft domains, which suppresses the production of amyloid beta (2010)
  • Author(s): Michinori Matsuo, Osamu Sano, Reiko Kato
  • Organizer: The 27thNaito Conference "Membrane Dynamics and Lipid Biology [I]"
  • Place of Presentation: Sapporo
• [Presentation] Expression of ABCG1 or ABCG4 modulates raft domains, which suppresses the production of amyloid beta (2010)
  • Author(s): Michinori Matsuo, Osamu Sano, Reiko Kato, Yuuji Shimizu and Kazumitsu Ueda
  • Organizer: 3rd FEBS special meeting "ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases"
  • Place of Presentation: Austria
• [Presentation] Mechanisms by which glia-derived apoE-containing lipoproteins stimulate axon elongation of CNS neurons (2010)
  • Author(s): Michinori Matsuo
  • Organizer: The thirteenth MEMBRANE RESEARCH FORUM
  • Place of Presentation: Kyoto
• [Presentation] 脂質トランスポーターABCG1とABCG4がアミロイド前駆体タンパク質のプロセシングに与える効果 (2009)
  • Author(s): 松尾道憲、佐野修、加藤玲子、清水裕二、植田和光
  • Organizer: トランスポーター研究会第3回九州部会
  • Place of Presentation: 鹿児島
  • Year and Date: 2009-11-21
• [Book] 脂質のトランスポーター」 竹谷豊、薩秀夫、伊藤美紀子、武田英二編 『栄養・食品機能とトランスポーター (2011)
  • Author(s): 松尾道憲、千場智尋、植田和光
  • Publisher: 建帛社
• [Book] 「栄養と代謝物のトランスポーター-脂質-」 金井好克、竹島浩、森泰生、久保義弘編『トランスポートソームの世界-膜輸送研究の源流から未来へ-』 (2011)
  • Author(s): 松尾道憲、植田和光
  • Publisher: 京都廣川書店
• [Remarks]
  • URL: http://cseika-1.kais.kyoto-u.ac.jp/
• [Remarks]
  • URL: http://cseika-l.kais.kyoto-u.ac.jp/
• [Remarks]
  • URL: http://cseika-1.kais.kyoto-u.ac.jp/