| Budget Amount *help |
¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000)
Fiscal Year 2011: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2010: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2009: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
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| Research Abstract |
During ovulation process, ERK1/ 2 pathway increased the expression level of multiple kinds of genes in granulosa cells, which induced luteinization of granulosa cells, cumulus cell expansion and oocyte maturation. However, in in vitro culture system, the level of ERK1/ 2-target genes was lower than those in in vivo due to the low level of neuregulin 1. Neuregulin 1 that enhanced the phosphorylation of ERK1/ 2 was corded by Nrg1 which had two transcription start sites in both terminals. The micro-RNA that might be generated from antisense strand of Nrg1 via Dicer-dependent manner suppressed the transcription of sense strand. To rescue the low level of Nrg1 in in vitro, the addition of neuregulin improved oocyte developmental competence
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