The study for development of novel in vitro oocyte maturation technique generated from expression profile of micro-RNA during ovulation process
Project/Area Number |
21688019
|
Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Applied animal science
|
Research Institution | Hiroshima University |
Principal Investigator |
SHIMADA Masayuki 広島大学, 大学院・生物圏科学研究科, 准教授 (20314742)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000)
Fiscal Year 2011: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2010: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2009: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
|
Keywords | 排卵 / 顆粒膜細胞 / 転写制御 / シグナル伝達経路 / 受精 / 成長因子 / 卵 / 体外成熟培養 / 卵成熟 / シグナル伝達 / siRNA / 卵巣 / 減数分裂 / IVM / 遺伝子発現 / 卵丘細胞 / neureulin 1 / ERK1 / 2 |
Research Abstract |
During ovulation process, ERK1/ 2 pathway increased the expression level of multiple kinds of genes in granulosa cells, which induced luteinization of granulosa cells, cumulus cell expansion and oocyte maturation. However, in in vitro culture system, the level of ERK1/ 2-target genes was lower than those in in vivo due to the low level of neuregulin 1. Neuregulin 1 that enhanced the phosphorylation of ERK1/ 2 was corded by Nrg1 which had two transcription start sites in both terminals. The micro-RNA that might be generated from antisense strand of Nrg1 via Dicer-dependent manner suppressed the transcription of sense strand. To rescue the low level of Nrg1 in in vitro, the addition of neuregulin improved oocyte developmental competence
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Report
(4 results)
Research Products
(81 results)
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[Presentation] 卵胞発育と卵成熟2011
Author(s)
島田昌之
Organizer
生殖バイオロジー東京シンポジウム
Place of Presentation
東京都(招待講演)
Year and Date
2011-07-17
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[Book] 卵子学2011
Author(s)
森崇英、島田昌之
Total Pages
1187
Publisher
京大出版会
Related Report
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[Book] 卵子学2011
Author(s)
森崇英(総編集), 島田昌之(編集幹事)
Total Pages
1187
Publisher
京大出版会
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