Species-level Diversity of Epigenetic Regulation for Brain Function Mediated by Non-coding RNA

• Project/Area Number: 21688021
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Kyoto University
• Principal Investigator: IMAMURA Takuya 京都大学, 理学研究科, 准教授 (90390682)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000); Fiscal Year 2011: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2010: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000); Fiscal Year 2009: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
• Keywords: 非コードRNA / DNAメチル化 / 生物多様性 / エピジェネティック制御 / 脳

Research Abstract

Brains of mammalian species mutually differ in the morphology and functions, even those of human and chimpanzee that share~ 98% nucleotide homologies. We have reported promoter-associated noncoding RNA(pancRNA) that acts to activate downstream gene expression through DNA demethylation. This study shows that DNA elements inserted into gene promoter regions of a certain species have provided the templates for species-specific panc RNAs.

Research Products

• [Journal Article] Epigenetic setting for long-term expression of estrogen receptor alpha and androgen receptor in cells (2011)
  • Author(s): Takuya Imamura
  • Journal Title: Hormones and Behavior Volume: Vol.59 Issue: 3 Pages: 345-352
  • DOI: 10.1016/j.yhbeh.2010.05.018
  • Peer Reviewed
• [Journal Article] Kei-ichiro Maeda Involvement of brain ketone bodies and the noradrenergic pathway in diabetic hyperphagia in rats (2011)
  • Author(s): Kinuyo Iwata, Mika Kinoshita, Shunji Yamada, Takuya Imamura, Yoshihisa Uenoyama, Hiroko Tsukamura
  • Journal Title: Journal of Physiological Sciences Volume: Vol.61 Issue: 2 Pages: 103-113
  • DOI: 10.1007/s12576-010-0127-6
  • Peer Reviewed
• [Journal Article] Single-stranded noncoding RNAs mediate local epigenetic alterations at gene promoters in rat cell lines (2011)
  • Author(s): Junko Tomikawa, Hiroko Shimokawa, Masahiro Uesaka, Naoki Yamamoto, Yuji Mori, Hiroko Tsukamura, Kei-ichiro Maeda, Takuya Imamura
  • Journal Title: Journal of Biological Chemistry Volume: Vol.286 Issue: 40 Pages: 34788-34799
  • DOI: 10.1074/jbc.m111.275750
  • Peer Reviewed
• [Journal Article] BV Cell-to species-level diversity of epigenetic setting for androgen receptor expression in mammals (2011)
  • Author(s): Masahiro Uesaka, Takuya Imamura
  • Journal Title: Journal of Steroids and Hormonal Science Volume: Vol.S2 Issue: S2 Pages: 4-4
  • DOI: 10.4172/2157-7536.s2-004
  • Peer Reviewed
• [Journal Article] Epigenetic setting for long-term expression of estrogen receptor alpha and androgen receptor in cells (2011)
  • Author(s): Takuya IMAMURA
  • Journal Title: Hormones and Behavior Volume: 59 Pages: 345-352
  • Peer Reviewed
• [Journal Article] Involvement of brain ketone bodies and the noradrenergic pathway in diabetic hyperphagia in rats (2011)
  • Author(s): Iwata T, Kinoshita M, Yamada S, Imamura T, Uenoyama Y, Tsukamura H, Maeda K
  • Journal Title: The Journal of Physiological Sciences Volume: 61 Pages: 103-113
  • NAID: 10029049665
  • Peer Reviewed
• [Presentation] Possible involvement of cell-state-specific promoter-associated noncoding RNAs in the epigenome formation of rat PC12 cells (2011)
  • Author(s): Yamamoto N, Hamazaki N, Uesaka M, Imamura T
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-16
• [Presentation] Species-specific pseudo gene insertions generate cis-acting RNA for promoter demethylation in the macaque (2011)
  • Author(s): Uesaka M, Nishimura O, Uno K, Ueda HR, Oishi T, Imai H, Agata K, Imamura T
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-16
• [Presentation] Species-specific pseudogene insertions generate cis-acting RNA for promoter demethylation in the macaque (2011)
  • Author(s): Masahiro Uesaka, Osamu Nishimura, Ken-ichiro Uno, Hiroki Ueda, Takao Oishi, Hiroo Imai, Kiyokazu Agata, Takuya Imamura
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-16
• [Presentation] マカクザルにおける偽遺伝子由来promoter-associated noncoding RNA(panc RNA)による種特異的転写活性化 (2011)
  • Author(s): 今村拓也、上坂将弘、西村理、大石高生、今井啓雄、阿形清和
  • Organizer: 第152回日本獣医学会大会
  • Place of Presentation: 大阪府立大学(堺市)
  • Year and Date: 2011-09-20
• [Presentation] マカクザルにおける偽遺伝子由来promoter-associated noncoding RNA (pancRNA)による種特異的転写活性化 (2011)
  • Author(s): 今村拓也、上坂将弘、西村理、大石高生、今井啓雄、阿形清和
  • Organizer: 第152回日本獣医学会大会
  • Place of Presentation: 大阪府立大学(堺市)
  • Year and Date: 2011-09-20
• [Presentation] エピゲノム制御に関連するpromoter-associated noncoding RNAの卵活性化依存的発現 (2011)
  • Author(s): 浜崎伸彦、上坂将弘、阿形清和、今村拓也
  • Organizer: 第104回日本繁殖生物学会大会
  • Place of Presentation: いわて県民交流センター・アイーナ(盛岡市)
  • Year and Date: 2011-09-16
• [Presentation] マカクザルにおける偽遺伝子由来promoter-associated noncoding RNA(panc RNA)による種特異的転写活性化 (2011)
  • Author(s): 上坂将弘、西村理、大石高生、今井啓雄、阿形清和、今村拓也
  • Organizer: 第104回日本繁殖生物学会大会
  • Place of Presentation: いわて県民交流センター・アイーナ(盛岡市)
  • Year and Date: 2011-09-16
• [Presentation] マカクザルにおける偽遺伝子由来promoter-associated noncoding RNA (pancRNA)による種特異的転写活性化 (2011)
  • Author(s): 上坂将弘、西村理、大石高生、今井啓雄、阿形清和、今村拓也
  • Organizer: 第104回日本繁殖生物学会大会
  • Place of Presentation: いわて県民交流センター・アイーナ(盛岡市)
  • Year and Date: 2011-09-16
• [Presentation] Species-specific promoter-associated noncoding RNA mediates DNA demethylation in macaques (2011)
  • Author(s): Imamura T, Uesaka M, Agata K
  • Organizer: Society for Molecular Biology and Evolution 2011
  • Place of Presentation: 京都大学(京都市)
  • Year and Date: 2011-07-27
• [Presentation] Species-specific promoter-associated noncoding RNA mediates DNA demethylation in macaques (2011)
  • Author(s): Takuya Imamura, Masahiro Uesaka, Kiyokazu Agata
  • Organizer: Society for Molecular Biology and Evolution 2011
  • Place of Presentation: 京都大学(京都市)(招待講演)
  • Year and Date: 2011-07-27
• [Presentation] Pseudo gene insertions may drive pancRNA acquisition during mammalisn evolution (2011)
  • Author(s): Uesaka M, Oishi T, Imai H, Nishimura O, Agata K, Imamura T
  • Organizer: 次世代シークエンサ現場の会第一回研究会
  • Place of Presentation: 熱海ニューフジヤマホテル(熱海市)
  • Year and Date: 2011-05-29
• [Presentation] Pseudogene insertions may drive pancRNA acquisition during mammalisn evolution (2011)
  • Author(s): Masahiro Uesaka, Takao Oishi, Hiroo Imai, Osamu Nishimura, Kiyokazu Agata, Takuya Imamura
  • Organizer: 次世代シークエンサ現場の会第一回研究会
  • Place of Presentation: 熱海ニューフジヤマホテル(熱海市)
  • Year and Date: 2011-05-29
• [Presentation] 浜崎伸彦、上坂将弘、阿形清和、今村拓也In vitro卵活性化依存的に発現するpromoter-associated noncoding RNAの同定 (2011)
  • Author(s): 浜崎伸彦、上坂将弘、阿形清和、今村拓也
  • Organizer: 第5回エピジェネティクス研究会年会
  • Place of Presentation: KKRホテル熊本(熊本)
  • Year and Date: 2011-05-20
• [Presentation] In vitro卵活性化依存的に発現するpromoter-associated noncoding RNAの同定 (2011)
  • Author(s): 浜崎伸彦、上坂将弘、阿形清和、今村拓也
  • Organizer: 第5回エピジェネティクス研究会年会
  • Place of Presentation: KKRホテル熊本(熊本)
  • Year and Date: 2011-05-20
• [Presentation] Potential of promoter-associated noncoding RNAs for epigenetic setting during differentiation (2010)
  • Author(s): Yamamoto N, Hamazaki N, Uesaka M, Shimokawa, H, Tsukamura H, Maeda K, Mori Y, Imamura T
  • Organizer: 16th International Conference of the International Society of Differentiation
  • Place of Presentation: 奈良県新公会堂(奈良市)
  • Year and Date: 2010-11-16
• [Presentation] ノンコーディングRNAによる性ステロイド受容体遺伝子発現とげっ歯類脳機能制御 (2010)
  • Author(s): 今村拓也、束村博子、前多敬一郎、森裕司
  • Organizer: 第103回日本繁殖生物学会
  • Place of Presentation: 北里大学獣医学部(十和田市)
  • Year and Date: 2010-09-02
• [Presentation] Single-stranded noncoding RNA mediate local epigenetic alterations at gene promoters in rat cell lines (2010)
  • Author(s): Imamura T, Tomikawa J, Shimokawa H, Uesaka M, Tsukamura H, Maeda K, Mori Y
  • Organizer: Keystone Symposium : RNA Silencing
  • Place of Presentation: キーストンリゾートホテル(米国コロラド州キーストン)
  • Year and Date: 2010-01-16
• [Presentation] Single-stranded noncoding RNA mediate local epigenetic alterations at gene promoters in rat cell lines (2010)
  • Author(s): Imamura T, Tomikawa J, Shimokawa H, Uesaka M, Tsukamura H, Maeda K, Mori Y
  • Organizer: Keystone Symposium : RNA Silencing
  • Place of Presentation: 米国コロラド州キーストン
  • Year and Date: 2010-01-16
• [Presentation] マウス・サル脳における種特異的promoter-associated noncoding RNAの同定 (2009)
  • Author(s): 上坂将弘、上田泰己、宇野健一郎、阿形清和、今村拓也
  • Organizer: 第102回日本繁殖生物学会
  • Place of Presentation: 近畿大学農学部(奈良県)
  • Year and Date: 2009-09-11
• [Remarks] 研究成果公開ウェブページ「ほ乳類の遺伝子ONをRNAにより制御する」
  • URL: http://www.a.u-tokyo.ac.jp/topics/2011/20111005-1.html
• [Remarks]
  • URL: http://www.a.u-tokyo.ac.jp/topics/2011/20111005-1.html
• [Remarks]
  • URL: http://gcoe.biol.sci.kyoto-u.ac.jp/gcoe/jpn/member/2008/01/imamura_takuya.php
• [Remarks]
  • URL: http://gcoe.biol.sci.kyoto-u.ac.jp/gcoe/eng/member/2008/02/imamura_takuya.php