Visualization of cellulase molecules at a solid/liquid interface using high-speed atomic force microscopy

• Project/Area Number: 21688023
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Boundary agriculture
• Research Institution: The University of Tokyo
• Principal Investigator: IGARASHI Kiyohiko 東京大学, 大学院・農学生命科学研究科, 准教授 (80345181)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥21,840,000 (Direct Cost: ¥16,800,000、Indirect Cost: ¥5,040,000); Fiscal Year 2010: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000); Fiscal Year 2009: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
• Keywords: セルラーゼ / セルロース / 高速原子間力顕微鏡 / 固液界面 / セロビオヒドロラーゼ

Research Abstract

We have succeeded in observing "traffic jams" of cellulase molecules on the surface of cellulose using high-speed atomic force microscopy. This "molecular congestion" is not only a new finding as basic science, but also is the first proof that this traffic jam of cellulase molecules is responsible for reducing the rate of cellulase hydrolysis. The findings of the present study provide a useful guide to developing an efficient system for the utilization of cellulose biomass near future.

Research Products

• [Journal Article] Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface (2011)
  • Author(s): Igarashi, K., Uchihashi, T., Koivula, A., Wada, M., Kimura, S., Okamoto, T., Penttila, M., Ando, T., and Samejima, M.
  • Journal Title: Science Volume: 333 Pages: 1279-1282
• [Journal Article] セルロース培養系においてエノキタケが生産する糖質加水分解酵素ファミリー7セロビオヒドロラーゼの解析 (2010)
  • Author(s): 石黒真希、堀友宣、吉田誠、高畠幸司、金子哲、五十嵐圭日子、鮫島正浩
  • Journal Title: 木材学会誌 Volume: 56 Pages: 397-404
  • NAID: 10029789394
  • Peer Reviewed
• [Journal Article] Molecular cloning of cDNAs encoding two glycoside hydrolase family 7 cellobiohydrolases from the basidiomycete Flammulina velutipes (2010)
  • Author(s): Ishiguro, M., Hori, T., Ishida, T., Yoshida, M., Takabatake, K., Kaneko, S., Igarashi, K., Samejima, M.
  • Journal Title: Plant Biotechnology Volume: 27 Pages: 273-281
  • NAID: 10026527649
  • Peer Reviewed
• [Journal Article] High-speed atomic force microscopy visualizes processive movement of Trichoderma reesei cellobiohydrolase I on crystalline cellulose (2009)
  • Author(s): Igarashi, K., Koivula, A., Wada, M., Kimura, S., Penttila, M., and Samejima, M.
  • Journal Title: J. Biol. Chem. Volume: 284 Pages: 36186-36190
• [Presentation] Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface (2011)
  • Author(s): Igarashi, K.
  • Organizer: Gordon Conference (Cellulosomes, Cellulases & Other Carbohydrate Modifying Enzymes)
  • Place of Presentation: ニューハンプシャー(米国)
  • Year and Date: 2011-07-24
• [Presentation] Single molecular observations of processive glycosidases by high-speed atomic force microscopy (2010)
  • Author(s): Igarashi, K., Uchihashi, T., Wada, M., Ando, T., Samejima, M.
  • Organizer: 第48回生物物理学会
  • Place of Presentation: 宮城県
  • Year and Date: 2010-09-20
• [Presentation] Single molecular observations of processive glycosidases by high-speed atomic force microscopy (2010)
  • Author(s): Kiyohiko Igarashi, Takayuki Uchihashi, Masahisa Wada, Toshio Ando, Masahiro Samejima
  • Organizer: 第48回生物物理学会
  • Place of Presentation: 東北大学(宮城県)
  • Year and Date: 2010-09-20
• [Presentation] プロセッシブ糖質加水分解酵素の分子機構 (2010)
  • Author(s): 五十嵐圭日子、和田昌久、鮫島正浩
  • Organizer: 第24回キチン・キトサンシンポジウム
  • Place of Presentation: 東京都
  • Year and Date: 2010-07-12
• [Presentation] プロセッシブ糖質加水分解酵素の分子機構 (2010)
  • Author(s): 五十嵐圭日子、和田昌久、鮫島正浩
  • Organizer: 第24回キチン・キトサンシンポジウム
  • Place of Presentation: 東京大学(東京都)
  • Year and Date: 2010-07-12
• [Presentation] Single molecule observations of processive glycosidases on crystalline substrates (2009)
  • Author(s): Igarashi, K.
  • Organizer: JST/CREST Symposium : WBMA'09 Watching Biomolecules in Action
  • Place of Presentation: 大阪府
  • Year and Date: 2009-12-15
• [Presentation] Single-molecule imaging of cellobiohydrolases on highly crystalline cellulose using high-speed atomic force microscopy (2009)
  • Author(s): Igarashi, K. Gordon
  • Organizer: Research Conference(Cellulosomes, Cellulases & Other Carbohydrate Modifying Enzymes)
  • Place of Presentation: 米国ニューハンプシャー州
  • Year and Date: 2009-07-29
• [Book] Visualization of cellobiohydrolase I from Trichoderma reesei moving on crystalline cellulose using high-speed atomic force microscopy. Methods Enzymol. (2012)
  • Author(s): Igarashi, K., Uchihashi, T., Koivula, A., Wada, M., Kimura, S., Penttila, M., Ando, T., Samejima, M.
• [Book] 次世代バイオエタノール生産の技術革新と事業展開 (2010)
  • Author(s): 五十嵐圭日子、鮫鳥正浩
  • Total Pages: 322
  • Publisher: 株式会社フロンティア出版
• [Book] 次世代バイオエタノール生産の技術革新と事業展開 (2010)
  • Author(s): 五十嵐圭日子、鮫島正浩
  • Total Pages: 322
  • Publisher: 株式会社フロンティア出版
• [Remarks]
  • URL: http://www.a.u-tokyo.ac.jp/topics/2010/20100122-1.html
• [Remarks]
  • URL: http://www.a.u-tokyo.ac.jp/topics/2011/20110902-1.html
• [Remarks]
  • URL: http://www.a.u-tokyo.ac.jp/topics/2011/20110902-1.html