Enhancement of plant photosynthesis by functional improvement of CO_2-fixing enzyme RuBisCO.

• Project/Area Number: 21688024
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Applied molecular and cellular biology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: ASHIDA Hiroki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (50362851)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000); Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2010: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000); Fiscal Year 2009: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
• Keywords: 光合成 / CO_2固定 / ルビスコ / 植物 / シアノバクテリア / アーキア / 枯草菌 / CO2固定 / 葉緑体形質転換 / ルビスコ祖先タンパク質 / 紅藻 / 葉緑体

Research Abstract

Residues for functional improvement of RuBisCO were identified by the results form studies of thermophilic cyanobacterial RuBisCO and RuBisCO ancestral protein from Bacillus. I found a pre-Calvin cycle for CO_2 fixation using RuBisCO in methanogenic archaeon. Improvement of plant RuBisCO by replacement with red-algal residue enabled to perform a normal photosynthetic CO_2-fixing with low RuBisCO level in tobacco. The genes necessary for the achievement of RuBisCO accumulation were identified for quantitative enhancement of RuBisCO to increase photosynthetic capacity.

Research Products

• [Journal Article] Increasing photosynthesis/RuBisCO and CO_2 concentrating mechanisms. Comprehensive Biotechnology (2011)
  • Author(s): Hiroki Ashida and Akiho Yokota
  • Journal Title: 2^<nd> edition Volume: 4 Pages: 165-176
• [Journal Article] Generation of transplastomic lettuce with enhanced growth and high yield (2010)
  • Author(s): Yaka Ichikawa, Masahiro Tamoi, Harumi Sakuyama, Takanori Maruta, Hiroki Ashida, Akiho Yokota and Shigeru Shigeoka
  • Journal Title: GM Crops Volume: 1 Pages: 322-326
  • Peer Reviewed
• [Journal Article] A DEAD-box protein is required for the formation of a hidden break in Arabidopsis chloroplast 23S rRNA (2010)
  • Author(s): Kenji Nishimura, Hiroki Ashida, Taro Ogawa and Akiho Yokota
  • Journal Title: Plant J Volume: 63 Pages: 766-777
  • Peer Reviewed
• [Journal Article] An evolutionally conserved Lys122 is essential for function in Rhodospirillum rubrum bona fide RuBisCO and Bacillus subtilis RuBisCO-like protein (2010)
  • Author(s): Toshihiro Nakano, Hiroki Ashida*, Eiichi Mizohata, Hiroyoshi Matsumura and Akiho Yokota
  • Journal Title: Biochem Biophys Res Commun Volume: 392 Pages: 212-216
  • Peer Reviewed
• [Journal Article] A DEAD-box protein is required for the formation of a hidden break in Arabidopsis chloroplast 23S rRNA. (2010)
  • Author(s): Nishimura K, Ashida H, Ogawa T, Yokota A
  • Journal Title: The Plant Journal Volume: 63 Pages: 766-777
  • Peer Reviewed
• [Journal Article] An evolutionally conserved Lys122 is essential for function in Rhodospirillum rubrum bona fide RuBisCO and Bacillus subtilis RuBisCO-like protein. (2010)
  • Author(s): Toshihiro Nakano,Hiroki Ashida^*, Eiichi Mizohata, Hiroyoshi Matsumura, Akiho Yokota
  • Journal Title: Biochemical and biophysical research communications 392 Pages: 212-216
  • Peer Reviewed
• [Journal Article] pfkB-type carbohydrate kinase family protein, NARA5, for massive expression of plastid-encoded photosynthetic genes in Arabidopsis thaliana (2009)
  • Author(s): Taro Ogawa, Kenji Nishimura, Takehiko Aoki, Hisabumi Takase, Ken-Ichi Tomizawa, Hiroki Ashida, and Akiho Yokota
  • Journal Title: Plant Physiol Volume: 151 Pages: 114-28
  • Peer Reviewed
• [Journal Article] Crystal structure of the apo, decarbamylated form of 2, 3-diketo-5-methylthiopentyl-1-phosp hate enolase from Bacillus subtilis. Acta Crystallogr (2009)
  • Author(s): Haruka Tamura, Yohtaro Saito, Hiroki Ashida, Yasushi Kai, Tsuyoshi Inoue, Akiho Yokota and Hiroyoshi Matsumura
  • Journal Title: D Biol. Crystallogr Volume: 65 Pages: 942-51
  • Peer Reviewed
• [Journal Article] Structural and functional similarities between a RuBisCO-like protein from Bacillus subtilis and photosynthetic RuBisCO (2009)
  • Author(s): Yohtaro Saito, Hiroki Ashida, Tomoko Sakiyama, Nicole Tandeau de Marsac, Antoine Danchin, Agnieszka Sekowska, and Akiho Yokota
  • Journal Title: J. Biol. Chem Volume: 284 Pages: 13256-13264
  • Peer Reviewed
• [Journal Article] pfkB-type carbohydrate kinase family protein, NARA5, for massive expression of plastid-encoded photosynthetic genes in Arabidopsis thaliana. (2009)
  • Author(s): Taro Ogawa, Kenji Nishimura, Takehiko Aoki, Hisabumi Takase, Ken-Ichi Tomizawa, Hiroki Ashida, Akiho Yokota
  • Journal Title: Plant Physiology 151 Pages: 114-128
  • Peer Reviewed
• [Journal Article] Crystal structure of the apo, decarbamylated form of 2,3-diketo-5-methyl thiopentyl-1-phosphate enolase from Bacillus subtilis (2009)
  • Author(s): Haruka Tamura, Yohtaro Saito, Hiroki Ashida, Yasushi Kai, Tsuyoshi Inoue, Akiho Yokota, Hiroyoshi Matsumura
  • Journal Title: Acta crystallographica. Section D, Biological crystallography 65 Pages: 942-951
  • Peer Reviewed
• [Journal Article] Structural and functional similarities between a RuBisCO-like protein from Bacillus subtilis and photosynthetic RuBisCO. (2009)
  • Author(s): Yohtaro Saito, Hiroki Ashida, Tomoko Sakiyama, Nicole Tandeaude Marsac, Antoine Danchin, Agnieszka Sekowska, Akiho Yokota
  • Journal Title: Journal of Biological Chemistry 284 Pages: 13256-13264
  • Peer Reviewed
• [Presentation] メタン産生アーキアMethanospirillum hungateiにおけるRuBisCOとPRKを利用した新規CO_2固定回路の解析 (2012)
  • Author(s): 河野卓成、遠藤千夏子、Mehrotora Sandhya、横田明穂、蘆田弘樹
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-25
• [Presentation] メタン産生アーキアMethanospirillum hungateiにおけるRuBisCOとPRKを利用した新規CO_2固定回路の解析 (2012)
  • Author(s): 河野卓成、遠藤千夏子、Mehrotora Sandhya、横田明穂、蘆田弘樹
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2012-03-25
• [Presentation] 葉緑体においてPEPカルボキシラーゼとPEPカルボキシキナーゼを過剰発現させることによるタバコ(C_3植物)の高CO_2条件下での水利用効率(WUE)の向上 (2012)
  • Author(s): 泉井桂、蘆田弘樹、橋詰恵丞、有川慶大、濱口祐子、横正健剛、横田明穂、秋田求
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-16
• [Presentation] 葉緑体においてPEPカルボキシラーゼとPEPカルボキシキナーゼを過剰発現させることによるタバコ(C_3植物)の高CO_2,条件下での水利用効率(WUE)の向上 (2012)
  • Author(s): 泉井桂、蘆田弘樹、橋詰恵丞、有川慶大、濱口祐子、横正健剛,横田明穂、秋田求
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-16
• [Presentation] メタン産生アーキアMethanospirillum hungateiにおけるRuBisCOとPRKを利用した新規CO_2固定回路の解析 (2011)
  • Author(s): 河野卓成、Mehrotra Sandhya、横田明穂、蘆田弘樹
  • Organizer: ユーグレナ研究会第27回研究集会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-11-12
• [Presentation] メタン産生アーキアMethanospirillum hungateiにおけるRuBisCOとPRKを利用した新規CO_2固定回路の解析 (2011)
  • Author(s): 河野卓成、Mehrotra Sandhya、横田明穂、蘆田弘樹
  • Organizer: ユーグレナ研究会第27回研究集会
  • Place of Presentation: 中部大学春日井キャンパス
  • Year and Date: 2011-11-12
• [Presentation] メチオニン欠乏環境で機能する枯草菌メチオニン再生経路酵素RuBisCO-like proteinと光合成CO_2固定酵素RuBisCOの比較研究 (2011)
  • Author(s): 蘆田弘樹
  • Organizer: 第84回日本生化学会大会シンポジウム「特殊環境下における微生物の潜在能力:酵素研究の新展開」
  • Place of Presentation: 京都
  • Year and Date: 2011-09-24
• [Presentation] メチオニン欠乏環境で機能する枯草菌メチオニン再生経路酵素RuBisCO-like proteinと光合成CO_2固定酵素RuBisCOの比較研究 (2011)
  • Author(s): 蘆田弘樹
  • Organizer: 第84回日本生化学会大会シンポジウム
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-09-24
• [Presentation] RuBisCO機能進化研究～RuBisCO-like proteinの解析を通して～ (2011)
  • Author(s): 蘆田弘樹
  • Organizer: 日本光合成学会若手の会
  • Place of Presentation: 京都
  • Year and Date: 2011-06-04
• [Presentation] RuBisCO機能進化研究～RuBisCO-like proteinの解析を通して～ (2011)
  • Author(s): 蘆田弘樹
  • Organizer: 日本光合成学会若手の会
  • Place of Presentation: 京都大学
  • Year and Date: 2011-06-04
• [Presentation] The molecular evolution of phosphoribulokinase for completion of the Calvin cycle (2011)
  • Author(s): Kono Takunari, Sandhya Mehrotora, Hiroki Ashida, Akiho Yokota
  • Organizer: Gordon Research Conferences CO2 Assimilation in Plants : Genome to Biome, Les Diablerets
  • Place of Presentation: Switzerland
  • Year and Date: 2011-05-30
• [Presentation] The molecular evolution of phosphoribulokinase for completion of the Calvin cycle (2011)
  • Author(s): Kono Takunari, Sandhya Mehrotora, Hiroki Ashida, Akiho Yokota
  • Organizer: Gordon Research Conferences CO_2 Assimilation in Plants : Genome to Biome
  • Place of Presentation: Les Diablerets, Switzerland
  • Year and Date: 2011-05-30
• [Presentation] Prospects for increasing plant photosynthesis by overcoming the limitations of CO_2-fixing enzyme, RuBisCO (2011)
  • Author(s): Hiroki Ashida、Akiho Yokota
  • Organizer: 平成22年度バイオインダストリー協会新資源生物変換研究会シンポジウム兼日本農芸化学会2011年度大会シンポジウム「炭素固定機構の分子基盤と産業応用への可能性を探る」
  • Place of Presentation: 京都
  • Year and Date: 2011-03-28
• [Presentation] Prospects for increasing plant photosynthesis by overcoming the limitations of CO_2-fixing enzyme, RuBisCO. (2011)
  • Author(s): 蘆田弘樹、横田明穂
  • Organizer: 日本農芸化学会2011年度大会 シンポジウム
  • Place of Presentation: 京都女子大学(開催予定が中止となり、要旨集で発表)
  • Year and Date: 2011-03-28
• [Presentation] アーキアにおける光合成カルビン回路の進化的完成の証拠 (2011)
  • Author(s): 河野卓成、Mehrotra Sandhya、横田明穂、蘆田弘樹
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-26
• [Presentation] アーキアにおける光合成カルビン回路の進化的完成の証拠 (2011)
  • Author(s): 河野卓成、Mehrotra Sandhya、横田明穂、蘆田弘樹
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都女子大学(開催予定が中止となり、要旨集で発表)
  • Year and Date: 2011-03-26
• [Presentation] RuBisCO-like protein H294の機能から予想される植物RuBisCOの活性化機構 (2011)
  • Author(s): 中野寿宏、横田明穂、蘆田弘樹
  • Organizer: 第52回日本植物生理学会
  • Place of Presentation: 東北
  • Year and Date: 2011-03-22
• [Presentation] 枯草菌RuBisCO-like protein His294の機能から予想される植物RuBisCOの活性化機構 (2011)
  • Author(s): 中野寿宏、横田明穂、蘆田弘樹
  • Organizer: 日本植物生理学会2011年度大会
  • Place of Presentation: 東北大学(開催予定が中止となり、要旨集で発表)
  • Year and Date: 2011-03-22
• [Presentation] phosphoribulokinaseの分子進化から視た光合成カルビンサイクルの起源 (2011)
  • Author(s): 河野卓成、メヒロートラサンディア、横田明穂、蘆田弘樹
  • Organizer: 第52回日本植物生理学会
  • Place of Presentation: 東北
  • Year and Date: 2011-03-20
• [Presentation] シロイヌナズナDEAD-box RNA helicase 39により23SリボソームRNAの導入されるhidden breakを介した葉緑体翻訳制御機構 (2011)
  • Author(s): 西村健司、蘆田弘樹、小川太郎、横田明穂
  • Organizer: 第52回日本植物生理学会
  • Place of Presentation: 東北
  • Year and Date: 2011-03-20
• [Presentation] phosphoribulokinaseの分子進化から視た光合成カルビンサイクルの起源 (2011)
  • Author(s): 河野卓成、Mehrotra Sandhya, 横田明穂、蘆田弘樹
  • Organizer: 日本植物生理学会2011年度大会
  • Place of Presentation: 東北大学(開催予定が中止となり、要旨集で発表)
  • Year and Date: 2011-03-20
• [Presentation] シロイヌナズナDEAD-box RNA helicase 39により23SリボソームRNAに導入されるhidden breakを介した葉緑体翻訳制御機構 (2011)
  • Author(s): 西村健司、蘆田弘樹、小川太郎、横田明穂
  • Organizer: 日本植物生理学会2011年度大会
  • Place of Presentation: 東北大学(開催予定が中止となり、要旨集で発表)
  • Year and Date: 2011-03-20
• [Presentation] 2酵素融合による新たな触媒能の獲得～テトラヒメナメチオニン代謝系で働くMtnBDの機能解析～ (2010)
  • Author(s): 中野寿宏、蘆田弘樹、横田明穂
  • Organizer: 2010年度日本農芸化学会関西支部大会
  • Place of Presentation: 奈良
  • Year and Date: 2010-10-03
• [Presentation] 2酵素融合による新たな触媒能の獲得～テトラヒメナ メチオニン代謝系で働くMtnBDの機能解析～ (2010)
  • Author(s): 中野寿宏、蘆田弘樹、横田明穂
  • Organizer: 日本農芸化学会関西支部大会
  • Place of Presentation: 近畿大学農学部
  • Year and Date: 2010-10-03
• [Presentation] A chloroplast RNase HI, NARA4, is essential for the massive accumulation of plastid-encoded proteins. (2010)
  • Author(s): Ogawa T, Ashida H, Nishimura K, Yokota A
  • Organizer: 2^<nd> International Symposium on Chloroplast Genomics and Genetic Engineering
  • Place of Presentation: National University of Ireland, Maynooth, Co.Kildare, Ireland
  • Year and Date: 2010-06-21
• [Presentation] A DEAD-box protein mediates the formation of a hidden break into chloroplast 23S rRNA in Arabodopsis. (2010)
  • Author(s): Nishimura K, Ashida H, Ogawa T, Yokota A
  • Organizer: 2^<nd> International Symposium on Chloroplast Genomics and Genetic Engineering
  • Place of Presentation: National University of Ireland, Maynooth, Co.Kildare, Ireland
  • Year and Date: 2010-06-21
• [Presentation] DEAD-boxタンパク質を介した葉緑体リボソームRNAへのhidden breakの導入 (2010)
  • Author(s): 西村健司、蘆田弘樹、小川太郎、横田明穂
  • Organizer: 第57回日本生化学会近畿支部例会
  • Place of Presentation: 奈良
  • Year and Date: 2010-05-22
• [Presentation] 枯草菌RuBisCO-like proteinのhistidine294は活性化に関与する (2010)
  • Author(s): 中野寿宏、蘆田弘樹、横田明穂
  • Organizer: 第57回日本生化学会近畿支部例会
  • Place of Presentation: 奈良
  • Year and Date: 2010-05-22
• [Presentation] RuBisCO-like proteinを用いた光合成CO_2固定酵素RuBisCOの分子進化研究 (2010)
  • Author(s): 蘆田弘樹、齋藤洋太郎、中野寿宏、田村はるか、松村浩由、横田明穂
  • Organizer: 第57回日本生化学会近畿支部例会
  • Place of Presentation: 奈良
  • Year and Date: 2010-05-22
• [Presentation] RuBisCO-like proteinを用いた光合成CO_2固定酵素RuBisCOの分子進化研究 (2010)
  • Author(s): 蘆田弘樹、齋藤洋太郎、中野寿宏、田村はるか、松村浩由、横田明穂
  • Organizer: 第57回日本生化学会近畿支部例会
  • Place of Presentation: 奈良先端科学技術大学院大学
  • Year and Date: 2010-05-22
• [Presentation] RuBisCO-like proteinのhistidine294は活性化に関与する (2010)
  • Author(s): 中野寿宏、蘆田弘樹、横田明穂
  • Organizer: 第57回日本生化学会近畿支部例会
  • Place of Presentation: 奈良先端科学技術大学院大学
  • Year and Date: 2010-05-22
• [Presentation] DEAD-boxタンパク質を介した葉緑体リボソームRNAへのhidden breakの導入 (2010)
  • Author(s): 西村健司、蘆田弘樹、小川太郎、横田明穂
  • Organizer: 第57回日本生化学会近畿支部例会
  • Place of Presentation: 奈良先端科学技術大学院大学
  • Year and Date: 2010-05-22
• [Presentation] 枯草菌RuBisCO-like proteinが保存する光合成RuBisCO触媒必須残基His294の役割 (2010)
  • Author(s): 中野寿宏、蘆田弘樹、横田明穂
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] 枯草菌RuBisCO-like proteinが保存する光合成RuBisCO触媒必須残基His294の役割 (2010)
  • Author(s): 中野寿宏、盧田弘樹、横田明穂
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2010-03-29
• [Presentation] Expression of the functional human thioredoxin-1 protein in lettuce chloroplasts (2010)
  • Author(s): Hiroki Ashida
  • Organizer: 2^<nd> International Symposium on Chloroplast Genomics and Genetic Engineering
  • Place of Presentation: Ireland
• [Presentation] A DEAD-box protein mediates the formation of a hidden break into chloroplast 23S rRNA in Arabodopsis (2010)
  • Author(s): Kenji Nishimura, Hiroki Ashida, Taro Ogawa and Akiho Yokota
  • Organizer: 2^<nd> International Symposium on Chloroplast Genomics and Genetic Engineering
  • Place of Presentation: Ireland
• [Presentation] A chloroplast RNase HI, NARA4, is essential for the massive accumulation of plastid-encoded proteins (2010)
  • Author(s): Taro Ogawa, Hiroki Ashida, Kenji Nishimura and Akiho Yokota
  • Organizer: 2^<nd> International Symposium on Chloroplast Genomics and Genetic Engineering
  • Place of Presentation: Ireland
• [Presentation] An evolutionally conserved lysine 134 is essential for the function in Rhodospirillum rubrum RuBisCO and RuBisCO-like protein from Bacillus subtilis (2009)
  • Author(s): Toshihiro Nakano, Hiroki Ashida, Akiho Yokota
  • Organizer: 第20回関西光合成研究会
  • Place of Presentation: 奈良
  • Year and Date: 2009-11-21
• [Presentation] DEAD-boxタンパク質による葉緑体リボソームRNAへのhidden breakの導入 (2009)
  • Author(s): 西村健司、蘆田弘樹、小川太郎、横田明穂
  • Organizer: 第20回関西光合成研究会
  • Place of Presentation: 奈良
  • Year and Date: 2009-11-21
• [Presentation] RuBisCOを焦点とした光合成タンパク質高蓄積機構におけるNARA遺伝子の位置付け (2009)
  • Author(s): 小川太郎、西村健司、蘆田弘樹、横田明穂
  • Organizer: 第20回関西光合成研究会
  • Place of Presentation: 奈良
  • Year and Date: 2009-11-21
• [Presentation] 植物RuBisCOの高蓄積を担うNARA遺伝子の分子遺伝学的解析 (2009)
  • Author(s): 小川太郎、西村健司、蘆田弘樹、横田明穂
  • Organizer: 日本農芸化学会関西・中四国・西日本支部、日本栄養・食糧学会九州・沖縄支部および日本食品科学工学会西日本支部合同沖縄大会
  • Place of Presentation: 沖縄
  • Year and Date: 2009-10-30
• [Presentation] 檀物RuBisCOの高蓄積を担うNARA遺伝子の分子遺伝学的解析 (2009)
  • Author(s): 小川太郎、西村健司、盧田弘樹、横田明穂
  • Organizer: 日本農芸化学会関西・中四国・西日本支部、日本栄養・食糧学会九州・沖縄支部および日本食品科学工学会西日本支部合同沖縄大会
  • Place of Presentation: 琉球大学千原キャンパス
  • Year and Date: 2009-10-30
• [Presentation] pfkB-type carbohydrate kinase family protein, NARA5 is essential for the active expressions of plastid-encoded photosynthetic genes in Arabidopsis thaliana. (2009)
  • Author(s): Taro Ogawa, Kenji Nishimura, T Aoki, Hiroki Ashida, Akiho Yokota
  • Organizer: Plant Biology 2009 Joint Annual Meeting of the American Society of Plant Biologists and the Phycological Society of America
  • Place of Presentation: Hawaii
• [Presentation] A DEAD-box RNA helicase RH39 is required for the chloroplast rRNA processing in Arabidopsis thaliana (2009)
  • Author(s): Kenji Nishimura, Taro Ogawa, Hiroki Ashida, Akiho Yokota
  • Organizer: Plant Biology 2009 Joint Annual Meeting of the American Society of Plant Biologists and the Phycological Society of America
  • Place of Presentation: Hawaii
• [Presentation] A DEAD-box RNA helicase RH39 is required for the chloroplast rRNA processing in Arabidopsis thaliana. (2009)
  • Author(s): Kenji Nishimura, Taro Ogawa, Hiroki Ashida, Akiho Yokota
  • Organizer: Plant Biology 2009 Joint Annual Meeting of the American Society of Plant Biologists and the Phycological Society of America
  • Place of Presentation: Honolulu, Hawaii
• [Presentation] pfkB-type carbohydrate kinase family protein, NARA5, is essential for the active expressions of plastid-encoded photosynthetic genes in Arabidopsis thaliana. (2009)
  • Author(s): Taro Ogawa, Kenji Nishimura, Takehiko Aoki, Hiroki Ashida, Akiho Yokota
  • Organizer: Plant Biology 2009 Joint Annual Meeting of the American Society of Plant Biologists and the Phycological Society of America
  • Place of Presentation: Honolulu, Hawaii
• [Book] Increasing photosynthesis/RuBisCO and CO_2 concentrating mechanisms. In : Comprehensive Biotechnology, 2^<nd> edition, 165-176 (2011)
  • Author(s): Hiroki Ashida, Akiho Yokota
  • Total Pages: 12
  • Publisher: Pergamon
• [Book] Comprehensive Biotechnology, 2^<nd> edition, Increasing photosynthesis/RuBisCO and CO_2 concentrating mechanism. (2011)
  • Author(s): Ashida H, Yokota A
  • Publisher: Elsevier(in Press)
• [Book] 光合成CO_2固定酵素RuBisCO誕生の秘密. RuBisCO-like proteinが隠し持つRuBisCOとしての潜在能力.化学と生物 (2010)
  • Author(s): 齋藤洋太郎、蘆田弘樹
• [Remarks]
  • URL: http://bsw3.naist.jp/yokota/research_ashida.html