| Budget Amount *help |
¥28,080,000 (Direct Cost: ¥21,600,000、Indirect Cost: ¥6,480,000)
Fiscal Year 2010: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2009: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
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| Research Abstract |
In this study, to investigate the physiological role of NML in vivo, we generated mice with a targeted deletion of the gene coding NML (NML-KO mice). The most of NML-KO mice (90%) were embryonic lethal. We generated mouse embryonic fibroblasts (MEF) from NML-KO embryos. We found that metabolites level of glycolysis and TCA cycle in NML-KO MEF have been different from those in WT MEF using the metabolome analysis by CE-MS. Theses result suggested that NML is related to the regulation of glucose metabolism. Next, we evaluated the glucose metabolism of the surviving NML-KO mice. The surviving NML-KO mice showed the increase of serum glucose levels and the decrease of serum insulin levels under the chow diet compared with WT mice. These results showed the suppression of insulin secretion from pancreatic beta cells in NML-KO mice.
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