Structural studies on influenza viral RNA polymerase for designing new anti-viral drug

• Project/Area Number: 21689014
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Yokohama City University
• Principal Investigator: OBAYAHI Eiji Yokohama City University, 大学院・生命ナノシステム科学研究科, 特任助教 (50321740)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2010: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000); Fiscal Year 2009: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
• Keywords: インフルエンザ / RNAポリメラーゼ / 構造解析 / 立体構造解析

Research Abstract

As one of the measures to the menace of the new influenza virus appearance, I performed structural studies for designing new anti-influenza viral drug. Because a compound which can inhibit the function of viral RNA polymerase playing a central role in the replication of the influenza virus will be a good candidate, I solved a crystal structure of the RNA polymerase. In addition, using purified RNA polymerase in this study, I succeeded in the making of the antibody which suppressed a function of RNA polymerase. Drug development based on these results is expected in future.

Research Products

• [Journal Article] インフルエンザウイルスRNAポリメラーゼの構造解析と創薬研究 (2010)
  • Author(s): 杉山佳奈子、尾林栄治、吉田尚史、朴三用
  • Journal Title: 日本結晶学会誌 52巻 Pages: 271-278
  • NAID: 10027673105
  • Peer Reviewed
• [Journal Article] インフルエンザウイルスRNAポリメラーゼの構造解析と創薬研究 (2010)
  • Author(s): 杉山佳奈子、尾林栄治、吉田尚史、朴三用
  • Journal Title: 日本結晶学会誌 Volume: 52 Pages: 271-278
  • NAID: 10027673105
  • Peer Reviewed
• [Journal Article] Structural insight into the essential PB1-PB2 subunit contact of the influenza virus RNA polymerase (2009)
  • Author(s): K.Sugiyama, E.Obayashi, A.Kawaguchi, Y.Suzuki, J.R.H.Tame, K.Nagata, S.-Y.Park
  • Journal Title: EMBO Journal Vol.28 Pages: 1803-1811
  • Peer Reviewed
• [Journal Article] インフルエンザ増殖の謎 (2009)
  • Author(s): 尾林栄治、朴三用、ついに解明!
  • Journal Title: 化学 64巻 Pages: 25-29
• [Journal Article] Structural insight into the essential PB1-PB2 subunit contact of the influenza virus RNA polymerase (2009)
  • Author(s): K.Sugiyama, E.Obayashi, A.Kawaguchi, Y.Suzuki, J.R.H.Tame, K.Nagata, S.-Y.Park
  • Journal Title: EMBO Journal 28巻 Pages: 1803-1811
  • Peer Reviewed
• [Presentation] The structural study on influenza RNA polymerase for designing new anti-viral drug (2010)
  • Author(s): Eiji Obayashi
  • Organizer: The 10th conference of the Asian Crystallographic Association
  • Place of Presentation: BEXCO、釜山(韓国)(招待講演)
  • Year and Date: 2010-11-02
• [Presentation] The structural study on influenza RNA polymerase for designing new anti-viral drug (2010)
  • Author(s): Eiji Obayashi(尾林栄治)
  • Organizer: The 10^<th> conference of the Asian Crystallographic Association
  • Place of Presentation: BEXCO、釜山(韓国)(招待講演)
  • Year and Date: 2010-11-02
• [Presentation] インフルエンザウイルスのRNAポリメラーゼサブユニットPB1-PB2サブユニト間相互作用の解明 (2010)
  • Author(s): 杉山佳奈子、尾林栄治、川口敦史、鈴木由佳理、テイム ジェレミー、永田恭介、朴三用
  • Organizer: 第47回日本蛋白質科学会
  • Place of Presentation: 北海道大学
• [Presentation] インフルエンザウイルスのRNAポリメラーゼサブユニットPA-PB1サブユニト間相互作用の解明 (2010)
  • Author(s): 吉田尚史、尾林栄治、河合文啓、川口敦史、永田恭介、テイム ジェレミー、朴三用
  • Organizer: 第47回日本蛋白質科学会
  • Place of Presentation: 北海道大学
• [Presentation] インフルエンザウイルスのRNAポリメラーゼにおけるサブユニット間相互作用 (2009)
  • Author(s): 尾林栄治
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: アスティ徳島
  • Year and Date: 2009-10-30