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Clarification of mechanisms for hierarchical gene expression in RNA world and its application

Research Project

Project/Area Number 21689049
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionNagasaki University

Principal Investigator

MORI Ryoichi  長崎大学, 大学院・医歯薬学総合研究科, 助教 (30509310)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000)
Fiscal Year 2011: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2010: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2009: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Keywords免疫 / 炎症 / microRNA / 次世代シークエンサー / 皮膚創傷治癒 / miRNA / 組織修復 / アンチセンスオリゴ / 線維化 / DNAアレイ / 次世代シーケンサー
Research Abstract

Inflammation-related miRNAs might be involved in regulation of inflammatory response and tissue fibrosis. However, these genes have not been identified yet. In the present study, we identified wound-related known and unknown miRNAs using next generation sequencing. Then we identified inflammation-related miRNAs and clarified its functions using histological, cellular and molecular biological, and imaging analysis in vivo and in vitro.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (29 results)

All 2012 2011 2010 2009

All Journal Article (11 results) (of which Peer Reviewed: 10 results) Presentation (18 results)

  • [Journal Article] Reporter bioassay systems for the phenotypic screening of candidate drugs2011

    • Author(s)
      Chiba T, Tsuchiya T, Mori R, Shimokawa I
    • Journal Title

      Sensors

      Volume: 12 Pages: 1648-1656

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Release of TNF-αfrom macrophages is mediated by small GTPase Rab372011

    • Author(s)
      Mori R, Ikematsu K, Kitaguchi T, Kim SE, Okamoto M, Chiba T, Miyawaki A, Shimokawa I, Tsuboi T
    • Journal Title

      European Journal of Immunology

      Volume: 41 Pages: 3230-3239

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Acute stress response modified by modest inhibition of growth hormone axis : a potential machinery of the anti-aging effect of calorie restriction2011

    • Author(s)
      Komatsu T, Trindade LS, Chiba T, Hayashi H, Henmi T, Ushiroda Y, Mori R, Shimokawa I
    • Journal Title

      Mechanisms of Ageing and Development

      Volume: 132 Pages: 103-109

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Release of tumor necrosis factor-alpha from macrophages is mediated by small GTPase Rab372011

    • Author(s)
      Mori R, Ikematsu K, Kitaguchi T, Kim SE, Okamoto M, Chiba T, Miyawaki A, Shimokawa I, Tsuboi T
    • Journal Title

      European Journal of Immunology

      Volume: 41 Issue: 11 Pages: 3230-3239

    • DOI

      10.1002/eji.201141640

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Acute stress response modified by modest inhibition of growth hormone axis : apotential machinery of the anti-aging effect of calorie restriction2011

    • Author(s)
      Komatsu T, Trindade LS, Chiba T, Hayashi H, Henmi T, Ushiroda Y, Mori R, Shimokawa I
    • Journal Title

      Mechanisms of Ageing and Development

      Volume: 132 Issue: 3 Pages: 103-109

    • DOI

      10.1016/j.mad.2011.01.002

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] マクロファージからのサイトカイン分泌に関与する低分子量Gタンパク質Rabファミリーの機能解析2011

    • Author(s)
      森亮一、間所俊介、Kim Sang Eun、小松利光、千葉卓哉、坪井貴司、下川功
    • Journal Title

      Biomedical Gerontology

      Volume: 35 Pages: 33-36

    • NAID

      10029692166

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Development of Calorie Restriction Mimetics as Therapeutics for Obesity, Diabetes, Inflammatory and Neurodegenerative Diseases2010

    • Author(s)
      Chiba T, Tsuchiya T, Komatsu T, Mori R, Hayashi H, Shimokawa I
    • Journal Title

      Current Genomics

      Volume: 11 Pages: 562-567

    • NAID

      120006984859

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Wound-associated skin fibrosis : mechanisms and treatments based on modulating the inflammatory response. Endocrine2010

    • Author(s)
      Shaw TJ, Kishi K, Mori R
    • Journal Title

      Metabolic & Immune Disorders-Drug Targets

      Volume: 10 Pages: 320-330

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a bioassay to screen for chemicals mimicking the anti-aging effects of calorie restriction2010

    • Author(s)
      Chiba T, Tsuchiya T, Komatsu T, Mori R, Hayashi H, Shimano H, Spindler SR, Shimokawa I
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 401 Pages: 213-218

    • NAID

      120006984912

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] FoxO1 is involved in the antineoplastic effect of calorie restriction2010

    • Author(s)
      Yamaza H, Komatsu T, Wakita S, Kijogi C, Park S, Hayashi H, Chiba T, Mori R, Furuyama T, Mori N, Shimokawa I
    • Journal Title

      Aging Cell

      Volume: 9 Pages: 372-382

    • NAID

      120006984942

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Wound-associated skin fibrosis : mechanisms and treatments based on modulating the inflammatory response2010

    • Author(s)
      Shaw TJ, Kishi K, Mori R
    • Journal Title

      Endocr Metab Immune Disord Drug Targets

      Volume: Vol 10 Pages: 320-30

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Identification of differentially expressed circulating microRNA-complexed with argonaute2 in blood plasma of calorie restriction and high fat diet-induced obese mice by next generation sequencing2012

    • Author(s)
      森亮一
    • Organizer
      The 2nd Pusan-Nagasaki Joint Seminar on Aging Research
    • Place of Presentation
      Pusan, Koria
    • Year and Date
      2012-02-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Identification of differentially expressed circulating microRNA-complexed with argonaute2 in blood plasma of calorie restriction and high fat diet-induced obese mice by next generation sequencing2011

    • Author(s)
      Mori R
    • Organizer
      The 2nd Pusan-Nagasaki Joint Seminar on Aging Research
    • Place of Presentation
      Pusan, Korea
    • Year and Date
      2011-01-16
    • Related Report
      2011 Final Research Report
  • [Presentation] 皮膚創傷治癒過程における炎症反応特異的microRNAの同定2011

    • Author(s)
      森亮一、Kim Sang Eun、岡本百々子、千葉卓哉、下川功
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 皮膚創傷治癒過程における炎症反応特異的microRNAの同定2011

    • Author(s)
      森亮一
    • Organizer
      第3回日本RNAi研究会
    • Place of Presentation
      広島
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] マクロファージのサイトカイン分泌に関与する低分子量G蛋白質Rab familyの機能解析2011

    • Author(s)
      森亮一、間所俊介、Kim Sang Eun、小松利光、千葉卓哉、坪井貴司、下川功
    • Organizer
      第34回日本基礎老化学会
    • Place of Presentation
      東京
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Molecular mechanisms for TNFαsecretion from macrophages2011

    • Author(s)
      Mori R
    • Organizer
      Gordon Research Conferences-Tissue Repair & Regeneration
    • Place of Presentation
      New Hampshire, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] マクロファージにおけるtumor necrosis factor-αの分泌は低分子量G蛋白質Rab37によって制御される2011

    • Author(s)
      森亮一、千葉卓哉、下川功
    • Organizer
      第100回日本病理学会総会
    • Place of Presentation
      横浜
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Molecular mechanisms for TNFα secretion from macrophages2011

    • Author(s)
      森亮一
    • Organizer
      Gordon Research Conferences-Tissue Repair & Regeneration
    • Place of Presentation
      New Hampshire, USA
    • Related Report
      2011 Annual Research Report
  • [Presentation] 次世代シーケンサーを用いたカロリー制限及び肥満マウス血漿中に存在するmicroRNAの網羅的同定2010

    • Author(s)
      森亮一、Kim San Eun、兼松宗太郎、千葉卓哉、小松利光、鈴木穣、下川功
    • Organizer
      第2回RNAi研究会
    • Place of Presentation
      広島
    • Related Report
      2011 Final Research Report
  • [Presentation] Identification of differentially expressed microRNA in blood plasma of calorie restricted and obese mice by next generation sequencing2010

    • Author(s)
      Mori R
    • Organizer
      Asian Aging Core for Longevity Research and Education 2010
    • Place of Presentation
      Jeju Island, Korea
    • Related Report
      2011 Final Research Report
  • [Presentation] 次世代シーケンサーを用いたカロリー制限及び肥満マウス血漿中に存在するmicroRNAの網羅的同定2010

    • Author(s)
      森亮一、Kim San Eun、兼松宗太郎、千葉卓哉、小松利光、鈴木穣、下川功
    • Organizer
      第33回日本基礎老化学会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Final Research Report
  • [Presentation] デジタルトランスクリプトーム解析による炎症及び組織修復関連microRNAの網羅的同定2010

    • Author(s)
      森亮一、千葉卓哉、下川功
    • Organizer
      第99回日本病理学会総会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] デジタルトランスクリプトーム解析による炎症及び組織修復関連microRNAの網羅的同定2010

    • Author(s)
      森亮一
    • Organizer
      第99回 日本病理学会総会
    • Place of Presentation
      京王プラザホテル(東京)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 次世代シーケンサーを用いたカロリー制限及び肥満マウス血漿中に存在するmicroRNAの網羅的同定2010

    • Author(s)
      森亮一
    • Organizer
      第33回日本基礎老化学会
    • Place of Presentation
      野依記念学術交流館(名古屋)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Identification of differentially expressed microRNA in blood plasma of calorie restriction and obese mice by deep sequencing approach2010

    • Author(s)
      Ryoichi Mori
    • Organizer
      Asian Aging Core for Longevity 2010
    • Place of Presentation
      Neighborhood Hotel in Jeju (Koria)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 次世代シーケンサーを用いたカロリー制限及び肥満マウス血漿中に存在するmicroRNAの網羅的同定2010

    • Author(s)
      森亮一
    • Organizer
      第2回RNAi研究会
    • Place of Presentation
      グランドプリンス広島(広島)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Molecular mechanisms of cytokine secretion in macrophage2009

    • Author(s)
      森亮一
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] Molecular mechanisms of cytokine secretion in macrophages2009

    • Author(s)
      森亮一、池松和哉、下川功、坪井貴司
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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