Structural study of hydration water molecules in protein crystals by coupled use of neutron and X-ray structure analysis
| Project/Area Number |
21700329
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bioinformatics/Life informatics
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| Research Institution | Japan Atomic Energy Agency |
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Principal Investigator |
OHHARA Takashi Japan Atomic Energy Agency, 量子ビーム応用研究部門, 研究員 (60391249)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | バイオインフォマティックス / 中性子構造解析 / 量子ビーム / タンパク質水和水 / 不凍タンパク質 |
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| Research Abstract |
The author developed an efficient method to determine the structure of hydration water molecules in protein crystals without arbitrariness by coupled use of single crystal neutron and X-ray structure analysis. In addition, the author successfully clarified the role of hydration water molecules for the anti-freeze activity of an anti-freeze protein by a mutation study and coupled use of neutron and X-ray structure analysis.
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Report
(3 results)
Research Products
(39 results)
