A novel mechanism of dendritic spine formation by synapse-resident transcriptional coactivator

• Project/Area Number: 21700340
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Gunma University
• Principal Investigator: YAMAZAKI Hiroyuki Gunma University, 大学院・医学系研究科, 助教 (10334137)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 樹状突起スパイン / 樹状突起フィロポディア / 転写調節因子 / アクチン細胞骨格 / フィロポディア / 核

Research Abstract

In this study, we analyzed the role of SPIKAR, a novel transcriptional-coactivator, in dendritic spine formation. SPIKAR is localized in not only nucleus but also cytoplasm. We indicated that SPIKAR is involved in early phase of spine formation, especially in filopodia formation. SPIKAR-Knockout mice showed embryonic fatality, suggesting that SPIKAR has pivotal roles in ontogenesis as well as neuronal development.

Research Products

• [Journal Article] シナプスの細胞骨格 (2010)
  • Author(s): 山崎博幸、白尾智明
  • Journal Title: Clinical Neuroscience. Vol28 Pages: 858-861
• [Journal Article] Low accumulation of drebrin at glutamatergic postsynaptic sites on GABAergic neurons. (2010)
  • Author(s): Hanamura K, Mizui T, Kakizaki T, Roppongi RT, Yamazaki H, Yanagawa Y, Shirao T.
  • Journal Title: Neuroscience. 169 Pages: 1489-1500
  • Peer Reviewed
• [Journal Article] F-actin-binding protein drebrin regulates CXCR4 recruitment to the immune synapse. (2010)
  • Author(s): Perez-Martinez M, Gordon-Alonso M, Cabrero JR, Barrero-Villar M, Rey M, Mittelbrunn M, Lamana A, Morlino G, Calabia C, Yamazaki H, Shirao T, Vazquez J, Gonzalez-Amaro R, Veiga E, Sanchez-Madrid F.
  • Journal Title: J Cell Sci. 123 Pages: 1160-1170
  • Peer Reviewed
• [Journal Article] Chemico-genetic identification of drebrin as a regulator of calcium responses. (2010)
  • Author(s): Mercer JC, Qi Q, Mottram LF, Law M, Bruce D, Iyer A, Morales JL, Yamazaki H, Shirao T, Peterson BR, August A.
  • Journal Title: Int J Biochem Cell Biol. 42 Pages: 337-345
  • Peer Reviewed
• [Journal Article] Genetic disruption of the alternative splicing of drebrin gene impairs context-dependent fear learning in adulthood. (2010)
  • Author(s): Kojima N, Hanamura K, Yamazaki H, Ikeda T, Itohara S, Shirao T.
  • Journal Title: Neuroscience. 165 Pages: 138-150
  • Peer Reviewed
• [Journal Article] シナプスの細胞骨格 (2010)
  • Author(s): 山崎博幸
  • Journal Title: Clinical Neuroscience Volume: 28 Pages: 858-861
• [Journal Article] Low accumulation of drebrin at glutamatergic postsynaptic sites on GABAergic neurons (2010)
  • Author(s): Hanamura K
  • Journal Title: Neuroscience Volume: 169 Pages: 1489-1500
  • Peer Reviewed
• [Journal Article] F-actin-binding protein drebrin regulates CXCR4 recruitment to the immune synapse (2010)
  • Author(s): Perez-Martinez M
  • Journal Title: J Cell Sci Volume: 123 Pages: 1160-1170
  • Peer Reviewed
• [Journal Article] Chemico-genetic identification of drebrin as a regulator of calcium responses (2010)
  • Author(s): Mercer JC
  • Journal Title: The International Journal of Biochemistry & Cell Biology Vol.42 Pages: 337-345
  • Peer Reviewed
• [Journal Article] Genetic disruption of the alternative splicing of drebrin gene impairs context-dependent fear learning in adulthood (2010)
  • Author(s): Nobuhiko Kojima
  • Journal Title: Neuroscience VOL.165 Pages: 138-150
  • Peer Reviewed
• [Journal Article] Drebrin a knockout eliminates the rapid form of homeostatic synaptic plasticity at excitatory synapses of intact adult cerebral cortex. (2009)
  • Author(s): Aoki C, Kojima N, Sabaliauskas N, Shah L, Ahmed TH, Oakford J, Ahmed T, Yamazaki H, Hanamura K, Shirao T.
  • Journal Title: J Comp Neurol. 517 Pages: 105-121
  • Peer Reviewed
• [Journal Article] Activity of the AMPA receptor regulates drebrin stabilization in dendritic spine morphogenesis. (2009)
  • Author(s): Takahashi H, Yamazaki H, Hanamura K, Sekino Y, Shirao T.
  • Journal Title: J Cell Sci. 122 Pages: 1211-1219
  • Peer Reviewed
• [Journal Article] Drebrin E is involved in the regulation of axonal growth through actin-myosin interactions. (2009)
  • Author(s): Mizui T, Kojima N, Yamazaki H, Katayama M, Hanamura K, Shirao T.
  • Journal Title: J Neurochem. 109 Pages: 611-622
  • Peer Reviewed
• [Journal Article] Drebrin a knockout eliminates the rapid form of homeostatic synaptic plasticity at excitatory synapses of intact adult cerebral cortex (2009)
  • Author(s): Chye Aoki
  • Journal Title: Journal of Comparative Neurology VOL.517 Pages: 105-121
  • Peer Reviewed
• [Journal Article] Activity of the AMPA receptor regulates drebrin stabilization in dendritic spine morphogenesis (2009)
  • Author(s): Hideto Takahashi
  • Journal Title: Journal of Cell Science VOL.122 Pages: 1211-1219
  • Peer Reviewed
• [Journal Article] Drebrin E is involved in the regulation of axonal growth through actinmyosin interactions (2009)
  • Author(s): Toshiyuld Mizui
  • Journal Title: Journal of Neurochemistry VOL.109 Pages: 611-622
  • Peer Reviewed
• [Presentation] 新規ドレブリン結合タンパク質の同定とその機能解析 (2011)
  • Author(s): 山崎博幸、白尾智明
  • Organizer: 第1回放射線神経生物研究集会
  • Place of Presentation: 刀城会館(群馬県)
  • Year and Date: 2011-01-29
• [Presentation] 新規ドレブリン結合タンパク質の同定とその機能解析 (2011)
  • Author(s): 山崎博幸
  • Organizer: 第1回放射線神経生物研究集会
  • Place of Presentation: 刀城会館(前橋)
  • Year and Date: 2011-01-29
• [Presentation] 新規ドレブリン結合タンパク質の同定とその機能解析 (2009)
  • Author(s): 山崎博幸
  • Organizer: 第18回海馬と高次脳機能学会
  • Place of Presentation: 湯涌創作の森交流研修棟(石州県)
  • Year and Date: 2009-11-21
• [Presentation] The role of Spikar in cytoplasm and identification of its functional domain (2009)
  • Author(s): 山崎博幸
  • Organizer: 第52回日本神経化学大会
  • Place of Presentation: 伊香保ホテル天坊(群馬県)
  • Year and Date: 2009-06-23
• [Presentation] ドレブリン結合タンパク質Spikarは細胞質で樹状突起棘形成に関与する (2009)
  • Author(s): 山崎博幸、白尾智明
  • Organizer: 第24回神経組織の成長・再生・移植研究会
  • Place of Presentation: 伊香保ホテル天坊(群馬県)
  • Year and Date: 2009-06-21
• [Presentation] ドレブリン結合タンパク質 Spikar は細胞質で樹状突起棘形成に関与する (2009)
  • Author(s): 山崎博幸
  • Organizer: 第24回神経の成長・再生・移植研究会
  • Place of Presentation: 伊香保ホテル天坊(群馬県)
  • Year and Date: 2009-06-21
• [Presentation] 新規ドレブリン結合タンパク質の同定とその機能解析 (2009)
  • Author(s): 山崎博幸、竹田麗子、白尾智明
  • Organizer: 第18回海馬と高次脳機能学会
  • Place of Presentation: 金沢湯湧創作の森(石川県)
• [Presentation] Identification of nuclear-shuttling signals for spikar function (2009)
  • Author(s): 山崎博幸、児島伸彦、白尾智明
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場(愛知県)
• [Presentation] The role of Spikar in cytoplasm and identification of its functional domain (2009)
  • Author(s): 山崎博幸、児島伸彦、白尾智明
  • Organizer: 第52回日本神経化学会
  • Place of Presentation: 伊香保ホテル天坊(群馬県)