Development of motor functions by formation of local neural circuits

• Project/Area Number: 21700344
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: The University of Tokyo
• Principal Investigator: KOHSAKA Hiroshi 東京大学, 大学院・理学系研究科, 助教 (20431900)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 発生・発達・再生神経科学 / 神経回路 / 神経科学 / 光生理学 / 遺伝学 / 行動学 / イメージング / 制御 / 電気生理学 / 発生・分化

Research Abstract

Network of neurons generates variety of animal's activities such as moving, sensing and memorizing. While the properties of each neuron have been deeply examined, how assembly of neurons creates coherent and coordinated functions remains to be clarified. In this study, we dissected Drosophila larval motor circuits that generate spatiotemporal patterns of locomotion. We succeeded in identification of a group of interneurons(we named them "PMSI") which control the speed of the larval locomotion. By detailed analysis of PMSI using Ca imaging and optogenetics, we revealed some of the mechanisms how motor circuits regulate speed of locomotion.

Research Products

• [Journal Article] Development of larval motor circuits inDrosophila (2012)
  • Author(s): Kohsaka, H., Okusawa, S., Itakura, Y., Fushiki, A. and Nose, A.
  • Journal Title: Development, Growth andDifferentiation Volume: 54 Issue: 3 Pages: 408-419
  • DOI: 10.1111/j.1440-169x.2012.01347.x
  • Peer Reviewed
• [Journal Article] Development of larval motor circuits in Drosophila (2012)
  • Author(s): Kohsaka, H., Okusawa, S., Itakura, Y., Fushiki, A., Nose, A
  • Journal Title: Development Growth and Differentiation Volume: (in press)
  • NAID: 10031136913
  • Peer Reviewed
• [Journal Article] Optical dissection of neural circuits responsiblefor Drosophila larval locomotion withhalorhodopsin (2011)
  • Author(s): Inada, K., Kohsaka, H., Takasu, E., and Nose, A.
  • Journal Title: PLoS One 6 Volume: 6 Issue: 12 Pages: e29019-e29019
  • DOI: 10.1371/journal.pone.0029019
  • Peer Reviewed
• [Presentation] 時空間パターンを生み出すメゾ回路の作動原理の解明 (2011)
  • Author(s): 高坂洋史、能瀬聡直
  • Organizer: 新学術領域研究「メゾスコピック神経回路から探る脳の情報処理基盤」第2回領域会議
  • Place of Presentation: 熱海
  • Year and Date: 2011-10-26
• [Presentation] Functional and anatomical analysis of segmentally-arrayed interneurons that regulate locomotion in Drosophila (2011)
  • Author(s): Kohsaka, H., Takasu, E., Nose, A.
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都(招待講演)
  • Year and Date: 2011-09-21
• [Presentation] Optogenetic dissection of central circuitsunderlying larval locomotion in Drosophila (2011)
  • Author(s): Kohsaka, H, and Nose, A.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] ショウジョウバエ幼虫の運動開始に関与する介在神経細胞の探索 (2011)
  • Author(s): 澤井貴史、高坂洋史、能瀬聡直
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] 神経活動の可視化と光操作による運動制御回路の解析 (2011)
  • Author(s): 高坂洋史、能瀬聡直
  • Organizer: 第3回光塾
  • Place of Presentation: 東京
• [Presentation] Regulation of Drosophila larval locomotion bypropagating activity of local interneurons (2011)
  • Author(s): Kohsaka, H. and Nose, A.
  • Organizer: Cold Spring Harbor Meeting on "Neurobiologyof Drosophila"
  • Place of Presentation: Cold Spring Harbor, U. S. A.
• [Presentation] Role of sensory experience in functionaldevelopment of Drosophila motor circuits (2011)
  • Author(s): Fushiki, A., Kohsaka, H. and Nose, A.
  • Organizer: 2011 Cold Spring Harbor Asia Conference "Assembly, plasticity, dysfunction and repair ofneural circuits"
  • Place of Presentation: Dushu lake, China
• [Presentation] Functional and anatomical analysis ofsegmentally-arrayed interneurons that regulatelarval locomotion in Drosophila (2011)
  • Author(s): Kohsaka, H., Takasu, E. and Nose, A.
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] Segmentally-arrayed interneurons regulate theperistaltic locomotion of Drosophila larvae (2011)
  • Author(s): Kohsaka, H., Takasu, E. and Nose, A.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] Formation and plastic change of the terminals ofsensory feedback neurons involved in Drosophilalarval locomotion (2011)
  • Author(s): Okusawa, S., Kohsaka, H. and Nose, A.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] Search for interneurons that regulate larvallocomotion in Drosophila (2011)
  • Author(s): Itakura, Y., Kohsaka, H. and Nose, A.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] Role of sensory experience in functionaldevelopment of Drosophila motor circuits (2011)
  • Author(s): Fushiki, A., Kohsaka, H. and Nose, A.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] Imaging and manipulating neural activitieswithin the motor circuit of the Drosophila larvae (2011)
  • Author(s): Kohsaka, H., Nii, R., Nakagawa, Y. and Nose, A.
  • Organizer: 1^<st> Korea University-Tokyo University JointWorkshop
  • Place of Presentation: Tokyo
• [Presentation] Regulation of Drosophila larval locomotion by propagating activity of local interneurons (2011)
  • Author(s): Kohsaka, H., Nose, A.
  • Organizer: Cold Spring Harbor Meeting on "Neurobiology of Drosophila"
  • Place of Presentation: Cold Spring Harbor lab, USA
• [Presentation] Segmentally-arrayed interneurons regulate the peristaltic locomotion of Drosophila larvae (2011)
  • Author(s): Kohsaka, H., Takasu, E., Nose, A.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] 時空間パターンを生み出すメゾ回路の作動原理の解明 (2011)
  • Author(s): 高坂洋史、能瀬聡直
  • Organizer: 新学術領域研究「メゾスコピック神経回路から探る脳の情報処理基盤」
  • Place of Presentation: 東京
• [Presentation] Role of sensory experience in functional development of Drosophila motor circuits (2011)
  • Author(s): Fushiki, A., Kohsaka, H., Nose, A.
  • Organizer: 2011 Cold Spring Harbor Asia Conference "Assembly, plasticity, dysfunction and repair of circuits"
  • Place of Presentation: China
• [Presentation] Optogenetic dissection of central circuits underlying larval locomotion in Drosophila (2011)
  • Author(s): Kohsaka, H., Nose, A
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜(招待講演)
• [Presentation] Formation and plastic change of the terminals of sensory feedback neurons involved in Drosophila larval locomotion (2011)
  • Author(s): Okusawa, S., Kohsaka, H., Nose, A.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] Search for interneurons that regulate larval locomotion in Drosophila (2011)
  • Author(s): Itakura, Y, Kohsaka, H., Nose, A.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] Role of sensory experience in functional development of Drosophila motor circuits (2011)
  • Author(s): Fushiki, A., Kohsaka, H., Nose, A.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] Imaging and manipulating neural activities within the motorcircuit of the Drosophila larvae. (2011)
  • Author(s): Kohsaka, H., Nii, R., Nakagawa, Y., Inada, K., Nose, A
  • Organizer: 1^<st> Korea University-Tokyo University Joint Workshop.
  • Place of Presentation: 東京大学
• [Presentation] Imaging activity propagation in the Drosophilamotor circuits that regulate larval peristalsis (2010)
  • Author(s): Kohsaka, H., Nii, R., Nakagawa, Y., Inada, K. and Nose, A.
  • Organizer: Neuro2010神経科学学会シンポジウム
  • Place of Presentation: 神戸
• [Presentation] Temporal perturbation of neural acitivity inDrosophila larvae undergoing locomotion (2010)
  • Author(s): Inada, K., Kohsaka, H., Takasu, E. andNose, A.
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸
• [Presentation] Functional localization of neuronal componentscontrolling larval peristaltic movements inDrosophila (2010)
  • Author(s): Nakagawa, Y., Kohsaka, H. and Nose, A.
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸
• [Presentation] 神経回路の時空間活動パターンのモデル化 (2010)
  • Author(s): 高坂洋史
  • Organizer: 脳科学若手の会ワークショップ
  • Place of Presentation: 埼玉
• [Presentation] Optical control of Drosophila larval locomotionwith halorhodopsin (2010)
  • Author(s): Inada, K., Kohsaka, H., Takasu, E. and Nose, A.
  • Organizer: System neurobiology spring school
  • Place of Presentation: 京都
• [Presentation] Imaging activity propagation in the larval motor circuits that regulate peristalsis. (2010)
  • Author(s): Kohsaka, H., Nii, R., Nakagawa, Y., Inada, K., Nose, A
  • Organizer: NeuroFly 2010
  • Place of Presentation: Manchester, UK
• [Presentation] Imaging activity propagation in the Drosophila motor circuits that regulate larval peristalsis (2010)
  • Author(s): Kohsaka, H., Nii, R., Nakagawa, Y., Inada, K., Nose, A
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター
• [Presentation] Functional localization of neuronal components controlling larval peristaltic movements in Drosophila (2010)
  • Author(s): Nakagawa, Y., Kohsaka, H., Nose, A.
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター
• [Presentation] Temporal perturbation of neural activity in Drosophila larvae undergoing locomotion. (2010)
  • Author(s): Inada, K., Kohsaka, H., Takasu, E. Nose, A
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター
• [Presentation] ショウジョウバエ幼虫の運動回路の形成と可塑性 (2009)
  • Author(s): 高坂洋史
  • Organizer: 第17回東北大脳科学グローバルCOE若手フォーラム
  • Place of Presentation: 仙台
  • Year and Date: 2009-08-28
• [Presentation] ショウジョウバエ幼虫の中枢神経系におけるカルシウムイメージング: Imaging ofactivity propagation in the nerve cord ofDrosophila larvae (2009)
  • Author(s): 中川義章、仁井理恵、高坂洋史、能瀬聡直
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Imaging of activity propagation in the nerve cordof Drosophila larvae (2009)
  • Author(s): Kohsaka, H., Nii, R. and Nose, A.
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] Formation and plasticity of motor circuit inDrosophila (2009)
  • Author(s): Nose, A., Kohsaka, H., Tachi, Y., Okusawa, S
  • Organizer: 第32回日本神経科学大会シンポジウム
  • Place of Presentation: 名古屋
• [Presentation] Formation and plasticity of motor circuits inDrosophila (2009)
  • Author(s): Okusawa, S., Kohsaka, H., Tachi. Y. and Nose, A.
  • Organizer: 特定領域研究「統合脳」神経回路・分子脳科学領域合同夏班会議
  • Place of Presentation: 札幌
• [Presentation] Formation and plasticity of motor circuits inDrosophila (2009)
  • Author(s): Okusawa, S., Kohsaka, H., Tachi. Y. and Nose, A.
  • Organizer: Japan Drosophila Research Conference9(JDRC9)
  • Place of Presentation: 静岡
• [Presentation] Formation and plasticity of neural circuitsregulating peristaltic movements of Drosophilalarvae (2009)
  • Author(s): Kohsaka, H., Tachi, Y. and Nose, A.
  • Organizer: Constructing Neural Circuits meeting at JaneliaFarm
  • Place of Presentation: U. S. A.
• [Presentation] Formation and plasticity of neural circuits regulating peristaltic movements of Drosophila larv (2009)
  • Author(s): Kohsaka, H., Tachi, Y., Nose, A.
  • Organizer: "Constructing neural circuits" meeting at Janelia Farm
  • Place of Presentation: 米国、バージニア州アッシュバーン
• [Presentation] Imaging of activity propagation in the nerve cord of Drosophila larvae (2009)
  • Author(s): Kohsaka, H., Nii, R., Nose, A.
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] Formation and plasticity of motor circuit in Drosophila (2009)
  • Author(s): Nose, A., Kohsaka, H., Tachi, Y.
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] ショウジョウバエ幼虫の中枢神経系におけるカルシウムイメージング (2009)
  • Author(s): 中川義章、仁井理恵、高坂洋史、能瀬
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Remarks] 日本経済新聞(2011年3月20日)ミクロで観る生命、4.成長するハエの卵、神経回路ができる瞬間
• [Remarks]
  • URL: http://bio.phys.s.u-tokyo.ac.jp/