Analysis of the molecular mechanism regulating synaptic functions in the mouse cerebellar cortex using a system for conditional knockout in the cells constituting synapses.

• Project/Area Number: 21700348
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Niigata University
• Principal Investigator: ABE Manabu Niigata University, 脳研究所, 助教 (10334674)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 分子・細胞神経科学 / グルタミン酸受容体 / 分子・細胞神経化学

Research Abstract

The aim of this study was to reveal the relationship between molecular dynamics of glutamate receptor complex and regulation of synaptic function in cerebellar cortex. We first developed a system for conditional knockout in the cells constituting synapses. By analyzing the cell type-selective knockout mice, we found that the activity of glutamate receptors in Purkinje cells may regulate the presynaptic function at climbing and parallel fiber synapses and that β-catenin expressed in Bergmann glia may play an indispensable role in the formation of the laminar structure of cerebellum.

Research Products

• [Journal Article] Glutamate receptor dr δ2 isessential for input pathway-dependentregulation of synaptic AMPAR contents incerebellar Purkinje cells. (2011)
  • Author(s): 山崎美和子、宮崎太輔、畦地裕統、阿部学、他6名
  • Journal Title: Journal ofNeuroscience 31 Pages: 3362-3374
  • Peer Reviewed
• [Journal Article] Glutamate receptor d2 is essential for input pathway-dependent regulation of synaptic AMPAR contents in cerebellar purkinje cells. (2011)
  • Author(s): 山崎美和子
  • Journal Title: The Journal of Neuroscience Volume: 31 Pages: 3362-74
  • Peer Reviewed
• [Journal Article] Calpain 8/nCL-2 and calpain 9/nCL-4 constitute an activeprotease complex, G-calpain, involved ingastric mucosal defense. (2010)
  • Author(s): 秦勝志、阿部学、他7名
  • Journal Title: PLoS Genetics 6 : e1001040
  • Peer Reviewed
• [Journal Article] Theendocannabinoid 2-arachidonoylglycerolproduced by diacylglycerol lipase alphamediates retrograde suppression ofsynaptic transmission. (2010)
  • Author(s): 谷村あさみ、山崎真弥、橋本谷祐輝、内ヶ島基政、川田慎也、阿部学、他6名
  • Journal Title: Neuron 65 Pages: 320-327
  • Peer Reviewed
• [Journal Article] TARPs γ-2 and γ-7 are essential for AMPA receptor expression in the cerebellum. (2010)
  • Author(s): 山崎真弥
  • Journal Title: European Journal of Neuroscience Volume: 31 Pages: 2204-2220
  • Peer Reviewed
• [Journal Article] Calpain 8/nCL-2 and calpain 9/nCL-4 constitute an active protease complex, G-calpain, involved in gastric mucosal defense. (2010)
  • Author(s): 秦勝志
  • Journal Title: PLoS Genetics Volume: 6
  • Peer Reviewed
• [Journal Article] LPA4 regulates blood and lymphatic vessel formation during mouse embryogenesis. (2010)
  • Author(s): 住田隼一
  • Journal Title: Blood Volume: 116 Pages: 5060-70
  • Peer Reviewed
• [Journal Article] Chondroitin sulfate N-Acetylgalactosaminyltransferase-1 is required for normal cartilage development. (2010)
  • Author(s): 渡邊裕美
  • Journal Title: Biochemical Journal Volume: 432 Pages: 47-55
  • Peer Reviewed
• [Journal Article] The endocannabinoid 2-arachidonoylglycerol produced by diacylglycerol lipase alphamediates retrograde suppression of synaptic transmission. (2010)
  • Author(s): 谷村あさみ
  • Journal Title: Neuron 65 Pages: 320-327
  • Peer Reviewed
• [Journal Article] NMDA receptor GluN2B (GluR epsilon 2/NR2B) subunit is crucial for channel function, postsynaptic macromolecular organization, and actin cytoskeleton at hippocampal CA3 synapses. (2009)
  • Author(s): 明石馨
  • Journal Title: The Journal of Neuroscience 29 Pages: 10869-10882
  • Peer Reviewed
• [Presentation] Probing the physiological role of a novel CDC42 effector Protein. (2010)
  • Author(s): 上田-石原奈津実
  • Organizer: 第33回 日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-12-08
• [Presentation] Gene duplication alters expression and DNA methylation in Protocadher in cluster. (2010)
  • Author(s): 金子涼輔
  • Organizer: 第33回 日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-12-08
• [Presentation] Identification of G-calpain, agastrointestional-tract-specific calpain complex of calpain 8 and calpain 9, and its involvement in gastric mucosal defense. (2010)
  • Author(s): 秦勝志
  • Organizer: 第33回 日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-12-07
• [Presentation] The establishment and analysis of 26S proteasome conditional knockoutmice for the mechanisms of neurodegenerative diseases. (2010)
  • Author(s): 田代善崇
  • Organizer: 第33回 日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-12-07
• [Presentation] NMDA receptor GluN2B subunit is cruciaHbr channel function, postsynaptic macromolecular organization, and regulation of actin cytoskeleton at hippocampal CA3 synapses. (2010)
  • Author(s): 明石馨
  • Organizer: NEUROSCIENCE2010
  • Place of Presentation: アメリカ・サンディエゴ
  • Year and Date: 2010-11-16
• [Presentation] プロカドヘリン-α遺伝子の重複は発現とDNAメチル化を変化させる (2010)
  • Author(s): 金子涼輔
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-09-04
• [Presentation] CL3/CaMK 1gamma欠損マウスにおける組織学的・行動学的解析 (2010)
  • Author(s): 竹本-木村さやか
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-09-04
• [Presentation] CLICK-III遺伝子ノックアウト雄マウスの行動特性 (2010)
  • Author(s): 仲田真理子
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-09-04
• [Presentation] プルキンエ細胞選択的ノックアウトを用いた小脳AMPA受容体サブユニットの生理機能解析 (2010)
  • Author(s): 阿部学
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-09-03
• [Presentation] ジアシルグリセロールリパーゼαによって産出される2-アラキドノイルグリセロールが、逆行性のシナプス伝達抑圧を引き起こす (2010)
  • Author(s): 谷村あさみ
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-09-03
• [Presentation] 神経変性疾患モデル作製のための26Sプロテアソームコンディショナルノックアウトマウスの確立と解析 (2010)
  • Author(s): 田代善崇
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-09-03
• [Presentation] 海馬におけるAMPA受容体のシナプス発現とサブユニット構成 (2010)
  • Author(s): 山崎美和子
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-09-02
• [Presentation] Retrograde synaptic suppression in cerebellum, hippocampus and striatum is mediated by 2-arachidonoylglycerol produced by diacylglycerol lipase alpha. (2010)
  • Author(s): 谷村あさみ
  • Organizer: FENS Forum 2010
  • Place of Presentation: オランダ・アムステルダム
  • Year and Date: 2010-07-06
• [Presentation] ES細胞における相同組換え効率上昇法の開発 (2010)
  • Author(s): 阿部学
  • Organizer: 第57回日本実験動物学会総会
  • Place of Presentation: 京都府京都市
  • Year and Date: 2010-05-12
• [Presentation] 高効率相同組換え法はC57BL/6ES細胞RENKAの生殖細胞分化能に影響しない (2010)
  • Author(s): 夏目里恵
  • Organizer: 第57回日本実験動物学会総会
  • Place of Presentation: 京都府京都市
  • Year and Date: 2010-05-12
• [Presentation] ES細胞での相同組換え上昇に関与する因子の同定 (2009)
  • Author(s): 八矢幸大
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2009-12-11
• [Presentation] Analysis of the physiologicalfunction of cerebellar AMPA receptorsubunits using Purkinje cell-specificgene targeting system. (2009)
  • Author(s): 阿部学
  • Organizer: 第39回北米神経科学会大会
  • Place of Presentation: メリカ・シカゴ
  • Year and Date: 2009-10-18
• [Presentation] Analysis of the physiological function of cerebellar AMPA receptor subunits using Purkinje cell-specific gene targeting system. (2009)
  • Author(s): 阿部学
  • Organizer: 第39回北米神経科学会大会
  • Place of Presentation: McCormick Place convention center(Chicago)
  • Year and Date: 2009-10-18
• [Remarks]
  • URL: http://www.bri.niigata-u.ac.jp/cellular/
• [Remarks]
  • URL: http://www.bri.niigata-u.ac.jp/~cellular/
• [Patent(Industrial Property Rights)] ES細胞における相同組換え効率を改善する方法 (2010)
  • Inventor(s): 崎村建司、阿部学、八矢幸大
  • Industrial Property Rights Holder: 国立大学法人 新潟大学
  • Industrial Property Number: 2010-228524
  • Filing Date: 2010-10-08