Molecular mechanism of long-term synaptic plasticity at cerebellar inhibitory synapses

• Project/Area Number: 21700349
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Kyoto University
• Principal Investigator: KAWAGUCHI Shin-Ya Kyoto University, 大学院・理学研究科, 助教 (00378530)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: シナプス可塑性 / パッチクランプ / システム生物学 / GABARAP / CaMKII / GABA / シミュレーション / 抑制性シナプス / 可塑性 / GABA(A)受容体 / Ca^<2+>

Research Abstract

At GABAergic synapses on a cerebellar Purkinje neuron, long-term potentiation of inhibitory synaptic transmission mediated by GABA(A) receptors is induced by postsynaptic depolarization. Using electrophysiological and fluorescent imaging experiments coupled with systems biological simulation, this study revealed that neuronal activity-dependent sustained activation of CaMKII leads to stable modification of GABARAP self-association, contributing to the long-term maintenance of inhibitory synaptic LTP.

Research Products

• [Journal Article] Prediction and validation of a mechanism to control the threshold for inhibitory synaptic plasticity. (2009)
  • Author(s): Kitagawa, Y., Hirano, T., Kawaguchi, S.
  • Journal Title: Molecular Systems Biology 5 Pages: 280-280
  • Peer Reviewed
• [Journal Article] Prediction and validation of a mechanism to control the threshold for inhibitory synaptic plasticity. (2009)
  • Author(s): Yuichi Kitagawa
  • Journal Title: Molecular Systems Biology 5
  • Peer Reviewed
• [Presentation] Temporal sequence of Ca^2+ signals regulates long-term potentiation at inhibitory synapses on a cerebellar Purkinje neuron. (2010)
  • Author(s): Kawaguchi S., Hirano T.
  • Place of Presentation: Neuroscience2010 in San Diego, CA, USA
  • Year and Date: 2010-11-17
• [Presentation] Opposite roles of α and βCaMKII in LTP at inhibitory synapses on a Purkinje neuron. (2010)
  • Author(s): Nagasaki N., Hirano T., Kawaguchi S.
  • Place of Presentation: Neuroscience2010 in San Diego, CA, USA
  • Year and Date: 2010-11-17
• [Presentation] Temporal sequence of Ca^<2+> signals regulates long-term potentiation at inhibitory synapses on a cerebellar Purkinje neuron (2010)
  • Author(s): Shin-ya Kawaguchi
  • Organizer: 北米神経科学大会 Neuroscience2010
  • Place of Presentation: 米国サンディエゴ市コンベンションセンター
  • Year and Date: 2010-11-17
• [Presentation] Ca^2+シグナルの時間パターンによる小脳抑制性シナプス可塑性の制御 (2010)
  • Author(s): 川口真也, 平野丈夫
  • Organizer: 日本神経科学学会 Neuro2010
  • Place of Presentation: 兵庫県神戸市 神戸国際会議場
  • Year and Date: 2010-09-03
• [Presentation] Ca^<2+>シグナルの時間パターンによる小脳抑制性シナプス可塑性の制御 (2010)
  • Author(s): 川口真也
  • Organizer: 日本神経科学学会 Neuro2010
  • Place of Presentation: 兵庫県神戸市 神戸国際会議場
  • Year and Date: 2010-09-03
• [Presentation] Systems Biology of Inhibitory Synaptic Plasticity.Second International (2010)
  • Author(s): Kawaguchi S.
  • Organizer: Neuron to Synapse 2010 Meeting
  • Place of Presentation: in New York, U.S.A
  • Year and Date: 2010-06-07
• [Presentation] 小脳抑制性シナプス可塑性におけるCaMKIIサブユニット構成の役割 (2010)
  • Author(s): 長崎信博, 平野丈夫, 川口真也
  • Organizer: 日本生理学大会
  • Place of Presentation: 岩手県盛岡市 盛岡市民文化ホール
  • Year and Date: 2010-05-19
• [Presentation] CaMKIIサブユニット構成の違いによる小脳抑制性シナプス可塑性の調節 (2009)
  • Author(s): 長崎信博, 平野丈夫, 川口真也
  • Organizer: 日本神経科学学会Neuro2009
  • Place of Presentation: 愛知県名古屋市 名古屋国際会議場
  • Year and Date: 2009-09-16
• [Presentation] CaMKIIサブユニット構成の違いによる小脳抑制性シナプス可塑性の調節 (2009)
  • Author(s): 長崎信博
  • Organizer: 日本神経科学学会 Neuro2009
  • Place of Presentation: 愛知県名古屋市名古屋国際会議場
  • Year and Date: 2009-09-16
• [Book] Molecular Mechanism of Long-Term Plasticity at Cerebellar Inhibitory Synapses (2010)
  • Author(s): Kawaguchi, S., Hirano T.
  • Publisher: Inhibitory Synaptic Plasticity, Springer
• [Book] Inhibitory Synaptic Plasticity (2010)
  • Author(s): Shin-ya Kawaguchi, Tomoo Hirano
  • Publisher: Springer, Inc.
• [Remarks] ホームページ等
  • URL: http://www.neurosci.biophys.kyoto-u.ac.jp
• [Remarks]
  • URL: http://neurosci.biophys.kyoto-u.ac.jp