Molecular mechanisms underlying COUP-TFI/II-mediated temporal identity transition of neural stem cells

• Project/Area Number: 21700357
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: The Institute of Physical and Chemical Research (2011); Keio University (2009-2010)
• Principal Investigator: KANEDA Hayato 独立行政法人理化学研究所, 免疫転写制御研究グループ, 上級研究員 (40528212)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: グリア / 再生医学 / 時間特異性 / 神経幹細胞 / 神経発生 / 神経科学 / 脳・神経 / 再生 / competence / 幹細胞老化

Research Abstract

I succeeded in identification of the downstream effector of COUP-TFs and elucidation of the major molecular mechanisms underlying the neurogenic-to-gliogenic transition of developing neural stem cells. Furthermore, control of the effector enabled us to control the neural stem cell competence bi-directionally, neurogenic or gliogenic. These findings will greatly contribute to the clinical applications of neural stem cells for the treatment of CNS injury and disease.

Research Products

• [Journal Article] FRS2alpha regulates Erk levels to control a self-renewal target Hes1 and proliferation of FGF-responsive neural stem/progenitor cells (2010)
  • Author(s): Sato T, Shimazaki T, Naka H, Fukami S, Satoh Y, Okano H, Lax I, Schlessinger J & Gotoh N
  • Journal Title: Stem Cells Volume: 28 Pages: 1661-1673
  • Peer Reviewed
• [Journal Article] 神経新生からグリア新生へのスイッチ (2010)
  • Author(s): 仲・金田勇人,島崎琢也,岡野栄之
  • Journal Title: 実験医学 Volume: Vol.28, No.5(増刊)
• [Journal Article] Efficient generation of mature cerebellar Purkinje cells from mouse embryonic stem cells (2010)
  • Author(s): Tao O, Shimazaki T, Okada Y, Naka H, Kohda K, Yuzaki M, Mizusawa H & Okano H
  • Journal Title: J Neurosci Res Volume: 88 Pages: 234-247
  • Peer Reviewed
• [Journal Article] FRS2alpha regulates Erk levels to control a self-renewal target Hes1 and proliferation of FGF-responsive neural stem/progenitor cells. (2010)
  • Author(s): Sato T, Shimazaki T, Naka H, Fukami S, Satoh Y, Okano H, Lax I, Schlessinger J, Gotoh N.
  • Journal Title: Stem Cells Volume: 28(9) Pages: 1661-1673
  • Peer Reviewed
• [Journal Article] Efficient generation of mature cerebellar Purkinje cells from mouse embryonic stem cells. (2010)
  • Author(s): Tao O, Shimazaki T, Okada Y, Naka H, Kohda K, Yuzaki M, Mizusawa H, Okano H.
  • Journal Title: Journal of neuroscience research 88(2) Pages: 234-247
  • Peer Reviewed
• [Journal Article] 神経新生からグリア新生へのスイッチ (2010)
  • Author(s): 仲・金田勇人, 島崎琢也, 岡野栄之
  • Journal Title: 実験医学増刊 Vol. 28 No. 5
• [Presentation] Stem cell ageing biology no Su-Su-Me Invited lecture at Hokkaido University School of Dentistry (2010)
  • Author(s): Naka H
  • Organizer: Host : Professor Masato Tamura of Department of Oral Biochemistry and Molecular Biology
  • Year and Date: 2010-09-08
• [Presentation] Stem cell aging biologyのすゝめ (2010)
  • Author(s): 仲(金田)勇人
  • Organizer: 北海道大学大学院歯学研究セミナー
  • Place of Presentation: 北海道大学歯学部
  • Year and Date: 2010-09-08
• [Presentation] Neurogenesis-to-gliogenesis switch of neural stem cells (2010)
  • Author(s): Naka-Kaneda H, Nakamura S, Shimazaki T & Okano H
  • Organizer: The 8th Stem Cell Research Symposium
  • Place of Presentation: Awaji Yumebutai
  • Year and Date: 2010-05-13
• [Presentation] Neurogenesis-to-gliogenesis switch of neural stem cells (2010)
  • Author(s): Naka-Kaneda H, Nakamura S, Shimazaki T, Okano H.
  • Organizer: The 8th Stem Cell Research Symposium
  • Place of Presentation: 淡路夢舞台
  • Year and Date: 2010-05-13
• [Presentation] Neurogenesis-to-gliogenesis switch of neural stem cells (2010)
  • Author(s): Naka-Kaneda H, Nakamura S, Shimazaki T & Okano H
  • Organizer: Global COE Program Symposium 2010
  • Place of Presentation: Tokyo
  • Year and Date: 2010-02-26
• [Presentation] Temporal specification of neural stem cells (2009)
  • Author(s): Hayato Naka, Shiho Nakamura, Takuya Shimazaki, Hideyuki Okano
  • Organizer: 第7回幹細胞シンポジウム
  • Place of Presentation: 東京都港区六本木1-5-2泉ガーデンギャラリー
  • Year and Date: 2009-05-15
• [Presentation] Temporal specification of neural stem cells (2009)
  • Author(s): Naka H, Nakamura S, Shimazaki T & Okano H
  • Organizer: The 7th Stem Cell Research Symposium
  • Place of Presentation: Tokyo
  • Year and Date: 2009-03-15
• [Patent(Industrial Property Rights)] 神経分化促進剤 (2009)
  • Inventor(s): 岡野栄之、島崎琢也、仲勇人
  • Industrial Property Rights Holder: 学校法人慶應義塾
  • Filing Date: 2009-07-08
  • Overseas
• [Patent(Industrial Property Rights)] 神経分化促進剤 (2009)
  • Inventor(s): 岡野栄之、島崎琢也、仲勇人
  • Industrial Property Rights Holder: 学校法人慶應義塾
  • Filing Date: 2009-07-08
  • Overseas