RNA-binding protein Musashi1-mediated miRNA regulation

• Project/Area Number: 21700358
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Keio University
• Principal Investigator: KAWAHARA Hironori Keio University, 医学部, 助教 (00424177)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: Musashi1 / let-7 / Lin28 / 幹細胞 / ES細胞 / Musashil / Let-7 / 脳・神経 / 発現制御 / miRNA

Research Abstract

Musashi1 (Msi1) is an RNA-binding protein that is highly expressed in neural stem/progenitor cells (NS/PCs) as well as in other tissue stem cells. I showed that Msi1 works in concert with Lin28 to regulate post-transcriptional miRNA biogenesis in the cropping step, which occurs in the nucleus. Also, I found that Msi1 enhanced the localization of Lin28 to the nucleus and also inhibited the nuclear cropping step of another let-7 family miRNA, miR98.

Research Products

• [Journal Article] Musashil Cooperates in Abnormal Cell Lineage Protein 28 (Lin28)-mediated Let-7 Family MicroRNA Biogenesis in Early Neural Differentiation (2011)
  • Author(s): Hironori Kawahara, Yohei Ukada, Takao Imai, Akio Iwanami, Paul S.Mischel, Hideyuki Okano.
  • Journal Title: Journal of Biological Chemistry In press
  • Peer Reviewed
• [Journal Article] Musashi1 Cooperates in Abnormal Cell Lineage Protein 28 (Lin28)-mediated Let-7 Family MicroRNA Biogenesis in Early Neural Differentiation (2011)
  • Author(s): Hironori Kawahara, et al
  • Journal Title: Journal of Biological Chemistry Volume: (In press)
  • Peer Reviewed
• [Presentation] Ribonomics解析によるMusashiを介したmRNA regulonの同定 (2010)
  • Author(s): 今井貴雄、河原裕憲、河瀬聡、岩成宏子、望月康弘、児玉龍彦、浜窪隆雄、岡野栄之
  • Organizer: 第12回日本RNA学会
  • Place of Presentation: 東京都
  • Year and Date: 2010-07-28
• [Presentation] 分化制御の鍵をにぎる翻訳抑制複合体のNMR構造機能解析 (2010)
  • Author(s): 大山貴子、永田崇、田中志穂、飯倉裕之、河原裕憲、今井貴雄、岡野栄之、山崎俊夫、片平正人
  • Organizer: 第12回日本RNA学会
  • Place of Presentation: 東京都
  • Year and Date: 2010-07-28
• [Presentation] Characterization of RNA binding protein Musashi in zebrafish (2009)
  • Author(s): Masahiko Umei, Shinsuke Shibata, Hironori Kawahara, Shinji Makino, Hirotaka J Okano, Hideyuki Okano.
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-12-11
• [Presentation] NMR approaches to elucidate the molecular mechanism of translation inhibition by Musashil and PABP1 (2009)
  • Author(s): Hiroyuki Iikura, Shiho Tanaka, Keisuke Higuchi, Hironori Kawahara, Takao Imai, Hideyuki Okano, Takashi Nagata, Masato Katahira.
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-12-11
• [Presentation] RNA-binding protein Musashil regulates the function of let-7 miRNAs. (2009)
  • Author(s): Takao Imai, Hironori Kawahara, Satoshi Kawase, Hideyuki Okano.
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-12-09
• [Presentation] A contribution of Musashil to the regulation by Lin-28 in blocking miRNA processing. (2009)
  • Author(s): Hironori Kawahara, Takao Imai, Hideyuki Okano.
  • Organizer: 49th American Society for Cell Biology(米国細胞生物学会)
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2009-12-07
• [Presentation] A contribution of Musashil to the regulation by Lin-28 in blocking miRNA processing (2009)
  • Author(s): Hironori Kawahara, et al
  • Organizer: 49th American Society for Cell Biology(米国細胞生物学会)
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2009-12-07
• [Presentation] Musashil, PABP、mRNAが構成する翻訳抑制複合体体のNMP構造機能解析 (2009)
  • Author(s): 永田崇、大山貴子、杉山孝司、飯倉裕之、樋口敬介、河原裕憲、今井貴雄、岡野栄之、山崎俊夫、片平正人
  • Organizer: 第11回日本RNA学会
  • Place of Presentation: 新潟市
  • Year and Date: 2009-07-28
• [Presentation] A contribution of Musashil to the regulation by Lin-28 in blocking miRNA processing. (2009)
  • Author(s): Hironori Kawahara, Takao Imai, Hideyuki Okano.
  • Organizer: 7th STEM CELL Research Symposium
  • Place of Presentation: 東京都
  • Year and Date: 2009-05-15
• [Presentation] A contribution of Musashil to the regulation by Lin-28 in blocking miRNA rocessing (2009)
  • Author(s): Hironori Kawahara, et al
  • Organizer: 7^<th> STEM CELL Research Symposium
  • Place of Presentation: 東京都、日本
  • Year and Date: 2009-05-15