Comprehensive analysis of the higher-order auditory circuit in the fruit-fly brain

• Project/Area Number: 21700360
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Nagoya University (2011); Tokyo University of Pharmacy and Life Science (2009-2010)
• Principal Investigator: KAMIKOUCHI Azusa 名古屋大学, 理学研究科, 教授 (00525264)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 神経回路 / 脳 / 聴覚 / ショウジョウバエ / 二次聴覚神経 / feed-back / feed-forward / 神経伝達物質

Research Abstract

The aim of this research project is to clarify the neuronal basis that translate a given sound into meaningful neural signals in a brain. During the period of the project, we screened 3, 939 GAL4 enhancer-trap strains to visualizes the central auditory circuit in a fly brain. 3-dimensional high-resolution analysis of these brain established a map for the neural circuit formed by the 2nd-order auditory neurons. Such neuroanatomical map for auditory Drosophila brain.

Research Products

• [Journal Article] 分子遺伝学で探るショウジョウバエの聴覚と重力感覚の神経回路 (2011)
  • Author(s): 上川内あづさ
  • Journal Title: 生化学 Volume: 83(5) Pages: 399-402
  • NAID: 10029068486
  • Peer Reviewed
• [Journal Article] 聴覚神経系のシステムニューロバイオロジー:遺伝子発現誘導系を駆使した新たな研究戦略Systems neurobiology for auditory systems : a new strategy using a gene-expression induction system (2011)
  • Author(s): 上川内あづさ、伊藤啓
  • Journal Title: 実験医学 Volume: 29(4) Pages: 538-543
• [Journal Article] Transcuticular optical imaging of stimulus-evoked neural activities in the Drosophila PNS (2010)
  • Author(s): Kamikouchi A, Wiek R, Effertz T, Gopfert MC, Fiala A
  • Journal Title: Nat Protocols Volume: 5 Pages: 1229-1235
  • Peer Reviewed
• [Journal Article] Mechanical feedback amplification in Drosophila hearing is independent of synaptic transmission (2010)
  • Author(s): Kamikouchi A, Albert JT, Gopfert MC.
  • Journal Title: Eur J Neurosci Volume: 31 Pages: 697-703
  • Peer Reviewed
• [Journal Article] Methods for quantifying simple gravity sensing in Drosophila melanogaster (2010)
  • Author(s): Inagaki HK, Kamikouchi A, Ito K.
  • Journal Title: Nat Protocols Volume: 5 Pages: 20-25
  • Peer Reviewed
• [Journal Article] Protocol for quantifying sound-sensing ability of Drosophila melanogaster (2010)
  • Author(s): Inagaki HK, Kamikouchi A, Ito K.
  • Journal Title: Nat Protocols Volume: 5 Pages: 26-30
  • Peer Reviewed
• [Journal Article] ショウジョウバエの音と重力の受容システムの解明Analysis of the Drosophila gravity-and sound-sensing systems (2010)
  • Author(s): 上川内あづさ、伊藤啓
  • Journal Title: 生物物理 Volume: 50(6) Pages: 282-285
• [Journal Article] Transcuticular optical imaging of stimulus-evoked neural activities in the Drosophila PNS. (2010)
  • Author(s): Kamikouchi A, Wiek R, Effertz T, Gopfert MC, Fiala A.
  • Journal Title: Nat Protocols Volume: 5 Pages: 1229-1235
  • Peer Reviewed
• [Journal Article] ショウジョウバエの音と重力の受容システムの解明Analysis of the Drosophila gravity- and sound-sensing systems (2010)
  • Author(s): 上川内あづさ、伊藤啓
  • Journal Title: 生物物理 Volume: 50 Pages: 282-285
  • Peer Reviewed
• [Journal Article] Mechanical feedback amplification in Drosophila hearing is independent of synaptic transmission. (2010)
  • Author(s): Kamikouchi A, Albert JT, Gopfert MC.
  • Journal Title: Eur J Neurosci 31 Pages: 697-703
  • Peer Reviewed
• [Journal Article] Methods for quantifying simple gravity sensing in Drosophila melanogaster. (2010)
  • Author(s): Inagaki HK, Kamikouchi A, Ito K.
  • Journal Title: Nat Protocols 5 Pages: 20-25
  • Peer Reviewed
• [Journal Article] Protocol for quantifying sound-sensing ability of Drosophila melanogaster. (2010)
  • Author(s): Inagaki HK, Kamikouchi A, Ito K.
  • Journal Title: Nat Protocols 5 Pages: 26-30
  • Peer Reviewed
• [Journal Article] 解剖学・生理学・行動学の統合的解析モデルとしてのショウジョウバエを利用した音・重力・風情報の脳内処理機構の解明Application of Drosophila as an integrative neural model to understand how sound, gravity, and wind information are processed in the brain (2009)
  • Author(s): 上川内あづさ、稲垣秀彦、萬涼子、伊藤啓
  • Journal Title: 蛋白質核酸酵素 Volume: vol.54(14) Pages: 1817-1826
• [Journal Article] ショウジョウバエにおける音、重力、風検知の神経基盤The neural mechanism underlying sound, gravity and wind senses in Drosophila (2009)
  • Author(s): 上川内あづさ、稲垣秀彦、伊藤啓
  • Journal Title: 実験医学 Volume: 27(13) Pages: 2105-2108
• [Journal Article] 解剖学・生理学・行動学の統合的解析モデルとしてのショウジョウバエを利用した音・重力・風情報の脳内処理機構の解明 (2009)
  • Author(s): 上川内あづさ、稲垣秀彦、萬涼子、伊藤啓
  • Journal Title: 蛋白質核酸酵素 54(14) Pages: 1817-1826
• [Journal Article] ショウジョウバエにおける音、重力、風検知の神経基盤 (2009)
  • Author(s): 上川内あづさ、稲垣秀彦、伊藤啓
  • Journal Title: 実験医学 27(13) Pages: 2105-2108
• [Presentation] The auditory map in the fly brain. Gender Equality Committee symposium Trends in Neuroscience(S2-J-1-2) (2011)
  • Author(s): Kamikouchi A, Seki H, Mizuno H, Miyakawa H, Ito K, Morimoto T.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-14
• [Presentation] Anatomy of the secondary auditory neurons in the Drosophila brain (2011)
  • Author(s): Seki H, Ishiguro Y, Miyakawa H, Morimoto T, Kamikouchi A.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-14
• [Presentation] The auditory map in the fly brain (2011)
  • Author(s): Kamikouchi A, Seki H, Mizuno H, Miyakawa H, Ito K, Morimoto T
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜(招待講演)
  • Year and Date: 2011-09-14
• [Presentation] The gravity-and sound-sensing systems in the fruit fly. Comparative mechanobiology from monad to human S32-3 (2011)
  • Author(s): Kamikouchi A, Seki H, Mizuno H, Miyakawa H, Ito K, Morimoto T.
  • Organizer: 8th International Congress of Comparative Physiology and Biochemistry
  • Place of Presentation: 名古屋
  • Year and Date: 2011-06-03
• [Presentation] The gravity- and sound-sensing systems in the fruit fly (2011)
  • Author(s): Kamikouchi A, Seki H, Mizuno H, Miyakawa H, Ito K, Morimoto T
  • Organizer: 8th International Congress of Comparative Physiology and Biochemistry
  • Place of Presentation: 名古屋(招待講演)
  • Year and Date: 2011-06-03
• [Presentation] Identification of neurons that project to the secondary auditory center in the Drosophila brain (2010)
  • Author(s): Seki H, Uchida A, Ito K, Miyakawa H, Kamikouchi A. Morimoto T.
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-02
• [Presentation] Anatomy and function of the secondary auditory neurons AMMC-B1 in the fruit fly brain (2010)
  • Author(s): Kamikouchi A, Hikita K, Mizuno H, Sato R, Miyakawa H, Ito K, Morimoto T.
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-02
• [Presentation] Identification of neurons that project to the secondary auditory center in the Drosophila brain. (2010)
  • Author(s): Seki H, Uchida A, Ito K, Miyakawa H, Morimoto T, Kamikouchi A.
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター(兵庫県)
  • Year and Date: 2010-09-02
• [Presentation] Anatomy and function of the secondary auditory neurons AMMC-B1 in the fruit fly brain. (2010)
  • Author(s): Kamikouchi A, Hikita K, Mizuno H, Sato R, Miyakawa H, Ito K, Morimoto T.
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター(兵庫県)
  • Year and Date: 2010-09-02
• [Presentation] 重力,風検知の神経基盤 (2010)
  • Author(s): 上川内あづさ、稲垣秀彦、Gopfert MC、宮川博義、森本高子、伊藤啓ショウジョウバエにおける音
  • Organizer: 第115回日本解剖学会総会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-03-28
• [Presentation] ショウジョウバエにおける音,重力,風検知の神経基盤 (2010)
  • Author(s): 上川内あづさ、稲垣秀彦、Gopfert MC、宮川博義、森本高子、伊藤啓
  • Organizer: 第115回日本解剖学会総会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-03-28
• [Presentation] Systematic identification of higher-order auditory neurons in a fruitfly brain (2010)
  • Author(s): Kamikouchi A, Hikita K, Seki H, Uchida A, Sato R, Ito K, Miyakawa H, Morimoto T.
  • Organizer: 国際バイオリソースシンポジウム「ショウジョウバエ」
  • Place of Presentation: 京都
  • Year and Date: 2010-03-17
• [Presentation] Systematic screening of higher-order auditory neurons in the fruitfly brain (2010)
  • Author(s): Kamikouchi A, Hikita K, Seki H, Uchida A, Ito K, Miyakawa H, Morimoto T.
  • Organizer: CSH meeting"NEUROBIOLOGY OF DROSOPHILA"
  • Place of Presentation: Cold Spring Harbor
  • Year and Date: 2010-03-17
• [Presentation] Systematic identification of higher-order auditory neurons in a fruitfly braim. (2010)
  • Author(s): Kamikouchi A, Hikita K, Seki H, Uchida A, Sato R, Ito K, Miyakawa H, Morimoto T.
  • Organizer: 国際バイオリソースシンポジウム「ショウジョウバエ」
  • Place of Presentation: 京都
  • Year and Date: 2010-03-17
• [Presentation] The neural pathway for and gravity-sensing in Drosophila (2009)
  • Author(s): Kamikouchi A, Ito K, Miyakawa H, Morimoto T.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] The neural pathway for hearing and gravity-sensing in Drosophila. (2009)
  • Author(s): Kamikouchi A, Ito K, Miyakawa H, Morimoto T.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Systematic screening of higher-order auditory neurons in the fruitfly brain. (2009)
  • Author(s): Kamikouchi A, Hikita K, Seki H Uchida A, Ito K, Miyakawa H, Morimoto T.
  • Organizer: CSH meeting "NEUROBIOLOGY OF DROSOPHILA"
  • Place of Presentation: ニユーヨーク
  • Year and Date: 2009-10-01
• [Presentation] The neural circuits of Drosophila gravity sensing and hearing (2009)
  • Author(s): 上川内あづさ、稲垣秀彦、Effertz T, Fiala A,伊藤啓, Gopfert MC
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-17
• [Presentation] Systematic screening of higher-order auditory neurons in the Drosophila brain (2009)
  • Author(s): Kamikouchi A, Uchida A, Seki H, Hikita K, Ito K, Miyakawa H, Morimoto T.
  • Organizer: 第9回日本ショウジョウバエ研究集会
  • Place of Presentation: 静岡
• [Presentation] Systematic screening of higher-order auditory neurons in the Drosophila brain. (2009)
  • Author(s): Kamikouchi A, Uchida A, Seki H, Hikita K, Ito K, Miyakawa H, Morimoto T.
  • Organizer: 第9回日本ショウジョウバエ研究集会
  • Place of Presentation: 静岡
• [Book] 分子昆虫学ポストゲノムの昆虫研究, 分子生物学的手法を用いたショウジョウバエ聴覚系の解析(神村学ら編) (2009)
  • Author(s): 上川内あづさ
  • Publisher: 共立出版
• [Book] 「分子生物学的手法を用いたショウジョウバエ聴覚系の解析」In:分子昆虫学ポストゲノムの昆虫研究(神村学ら編) (2009)
  • Author(s): 上川内あづさ
  • Total Pages: 426
  • Publisher: 共立出版
• [Remarks]
  • URL: http://www.ls.toyaku.ac.jp/Life-Science/lcn-9/KamSites/kamJP.htm
• [Remarks]
  • URL: http://www.ls.toyaku.ac.jp/Life-Science/lcn-9/KamSites/kamEN.htm