Effect of transplantation of ischemia-induced neural stem cells through vascular niches

• Project/Area Number: 21700363
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Hyogo Medical University
• Principal Investigator: NAKAGOMI Takayuki 兵庫医科大学, 医学部, 講師 (80434950)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 神経再生 / 神経幹細胞 / 脳梗塞 / 血管ニッチ

Research Abstract

In the present study, we investigated the effect of transplantation of ischemia-induced neural stem cells through vascular niches. We found that endothelial cells support survival, proliferation and neuronal differentiation of transplanted ischemia-induced neural stem/progenitor cells after cerebral infarction.

Research Products

• [Journal Article] Leptomeningeal-Derived Doublecortin-Expressing Cells in Poststroke Brain (2012)
  • Author(s): Nakagomi T, Molnar Z, Taguchi A, Nakano-Doi A, Lu S, Kasahara Y, Nakagomi N, Matsuyama T.
  • Journal Title: Stem Cells and Development Volume: (in press)
  • Peer Reviewed
• [Journal Article] Glucocorticoid-induced TNF receptor-triggered T cells are key modulators for survival/death of neural stem/progenitor cells induced by ischemic stroke (2012)
  • Author(s): Takata M, Nakagomi T, Kashiwamura S, Nakano-Doi A, Saino O, Nakagomi N, Okamura H, Mimura O, Taguchi A, Matsuyama T.
  • Journal Title: Cell death and Differentiation Volume: 19 Pages: 756-767
  • Peer Reviewed
• [Journal Article] Neural stem cells after brain injury : Do they originate developmentally from neural tube, neural crest, or both? (2011)
  • Author(s): Nakagomi T and Matsuyama T.
  • Journal Title: Stem Cells Studies Volume: 1 Pages: 148-149
  • Peer Reviewed
• [Journal Article] Ischemia-induced neural stem/progenitor cells express pyramidal cells markers (2011)
  • Author(s): Clausen M, Nakagomi T, Nakano-Doi A, Saino O, Takata M, Taguchi A, Luiten P, Matsuyama T.
  • Journal Title: Neuroreport Volume: 22 Pages: 789-794
  • Peer Reviewed
• [Journal Article] Ischemia-induced neural stem/progenitor cells in the pia mater following cortical infarction (2011)
  • Author(s): Nakagomi T, Molnar Z, Nakano-Doi A, Taguchi A, Saino O, Kubo S, Clausen M, Yoshikawa H, Nakagomi N, Matsuyama T.
  • Journal Title: Stem Cells and Development Volume: 20 Pages: 2037-2051
  • Peer Reviewed
• [Journal Article] Ischemia-induced neural stem/progenitor cells in the pia mater following cortical infarction (2011)
  • Author(s): Nakagomi T, Taguchi A, Matsuyama T
  • Journal Title: Stem Cells Dev Volume: 20 Pages: 2037-51
  • Peer Reviewed
• [Journal Article] Clausen, M, Nakagomi T, Nakano-Doi A, Saino O, Takata M, Taguchi A, Luiten P, Matsuyama T. Ischemia-induced neural stem/progenitor cells express pyramidal cell markers. NeuroReport 2011 ; 22 : 789-794 (2011)
  • Author(s): Clausen, M, Nakagomi T, Taguchi A, Matsuyama T
  • Journal Title: NeuroReport Volume: 22 Pages: 789-794
  • Peer Reviewed
• [Journal Article] Glucocorticoid-induced TNF receptor-triggered T cells are key modulators for survival/death of neural stem/progenitor cells induced by ischemicstroke (2011)
  • Author(s): Takata M, Nakagomi T, Kashiwamura S, Taguchi A, Matsuyama T
  • Journal Title: Cell Death Differ Volume: 19 Pages: 756-67
  • Peer Reviewed
• [Journal Article] Cilostazol reduces the risk of hemorrhagic infarction after administration of tissue plasminogen activator in a murine stroke model (2011)
  • Author(s): Kasahara Y, Nakagomi T, Matsuyama T, Taguchi A
  • Journal Title: Stroke Volume: 43 Pages: 499-506
  • Peer Reviewed
• [Journal Article] Human umbilical cord provides a significant source of unexpanded mesenchymal stromal cells (2011)
  • Author(s): Kikuchi-Taura A, Taguchi A, Matsuyama T, Nakagomi T
  • Journal Title: Cytotherapy Volume: 14 Pages: 441-50
  • Peer Reviewed
• [Journal Article] Neural stem cells after brain injury : do they originate developmentally from neural tube, neural crest, or both? (2011)
  • Author(s): Nakagomi T, Matsuyama T
  • Journal Title: Stem Cell Studies Volume: 1:e21 Pages: 148-149
  • Peer Reviewed
• [Journal Article] Bone marrow mononuclear cells promote proliferation of endogenous neural stem cells through vascular niches after cerebral infarction (2010)
  • Author(s): Nakano-Doi A, Nakagomi T, Fujikawa M, Nakagomi N, Kubo S, Lu Shan, Yoshikawa H, Soma T, Taguchi A, Matsuyama T.
  • Journal Title: Stem Cells Volume: 28 Pages: 1292-1302
  • Peer Reviewed
• [Journal Article] Immunodeficiency reduces neural stem/progenitor cell-apoptosis and enhances neurogenesis in the cerebral cortex after stroke (2010)
  • Author(s): Saino O, Taguchi A, Nakagomi T, Nakano-Doi A, Kashiwamura S, Doe N, Soma T, Nakagomi N, Yoshikawa H, Stern DM, Okamura H, Matsuyama T.
  • Journal Title: J Neurosci Res Volume: 88 Pages: 2385-2397
  • Peer Reviewed
• [Journal Article] Injury-induced neural stem/progenitor cells in post-stroke human cerebral cortex (2010)
  • Author(s): Nakayama D, Matsuyama T, Ishibashi-Ueda H, Nakagomi T, Kasahara Y, Hirose H, Kikuchi-Taura A, Stern DM, Mori H, Taguchi A.
  • Journal Title: Eur J Neurosci Volume: 31 Pages: 90-98
  • Peer Reviewed
• [Journal Article] Injury-induced neural stem/progenitor cells in post-stroke human cerebral cortex. (2010)
  • Author(s): Nakayama D, Nakagomi T, et al
  • Journal Title: Eur J Neurosci Volume: 31 Pages: 90-98
  • Peer Reviewed
• [Journal Article] Bone marrow mononuclear cells promote proliferation of endogenous neural stem cells through vascular niches after cerebral infarction. (2010)
  • Author(s): Nakano-Doi A, Nakagomi T, et al
  • Journal Title: Stem Cells Volume: 28 Pages: 1292-1302
  • Peer Reviewed
• [Journal Article] Immunodeficiency reduces neural stem/progenitor cell-apoptosis and enhances neurogenesis in the cerebral cortex after stroke. (2010)
  • Author(s): Saino O, Nakagomi T, et al
  • Journal Title: J Neurosci Res Volume: 88 Pages: 2385-2397
  • Peer Reviewed
• [Journal Article] Telmisartan suppresses cerebral injury of transient, but not permanent focal ischemia in a murine model. (2010)
  • Author(s): Kasahara Y, Nakagomi T, et al
  • Journal Title: Brain Res Volume: 1340 Pages: 70-80
  • Peer Reviewed
• [Journal Article] A reproducible and simple model of permanent cerebral ischemia in CB-17 and SCID mice. (2010)
  • Author(s): Taguchi A, Nakagomi T, et al
  • Journal Title: J Exp Stroke Transl Med Volume: 3 Pages: 28-33
  • Peer Reviewed
• [Journal Article] Endothelial cells support survival, proliferation and neuronal differentiation of transplanted adult ischemia-induced neural stem/progenitor cells after cerebral infarction (2009)
  • Author(s): Nakagomi N, Nakagomi T, Kubo S, Nakano-Doi A, Saino O, Takata M, Yoshikawa H, Stern DM, Matsuyama T, Taguchi A.
  • Journal Title: Stem Cells Volume: 27 Pages: 2185-2195
  • Peer Reviewed
• [Journal Article] Isolation and characterization of neural stem/progenitor cells from post-stroke cerebral cortex in mice (2009)
  • Author(s): Nakagomi T, Taguchi A, Fujimori Y, Saino O, Nakano-Doi A, Kubo S, Gotoh A, Soma T, Yoshikawa H, Nishizaki N, Nakagomi N, Stern DM, Matsuyama T.
  • Journal Title: Eur J Neurosci Volume: 29 Pages: 1842-1852
  • Peer Reviewed
• [Journal Article] Isolation and characterization of neural stem/progenitor cells from post-stroke cerebral cortex in mice (2009)
  • Author(s): Nakagomi T, Matsuyama T et al
  • Journal Title: Eur J Neurosci 29 Pages: 1842-1852
  • Peer Reviewed
• [Journal Article] Endothelial cells support survival, proliferation and neuronal differentiation of transplanted adult ischemia-induced neural stem/progenitor cells after cerebral infarction (2009)
  • Author(s): Nakagomi N, Nakagomi T et al
  • Journal Title: Stem Cells 27 Pages: 2185-2195
  • Peer Reviewed
• [Journal Article] Circulating CD34-positive cells have prognostic value for neurologic function in patients with past cerebral infarction (2009)
  • Author(s): Taguchi A, Nakagomi T et al
  • Journal Title: J Cereb Blood Flow Metab 29 Pages: 34-38
  • Peer Reviewed
• [Presentation] Expression of neural stem cells induced by transient cortical ischemia in mice (2011)
  • Author(s): Momota Y, Nakagomi T, Nakano-Doi A, Saino O, Taguchi A, Takata M, Tsukamoto Y, Miyamae M, Kotani J, Matsuyama T.
  • Organizer: XXXth International Symposium on Cerebral Blood Flow, Metabolism and Function & Xth International Conference on Quantification of Brain Function and PET
  • Place of Presentation: Barcelona, Spain
• [Presentation] Ischemia-induced neural stem/progenitor cells express pyramidal cell-markers in vivo and in vitro (2011)
  • Author(s): Clausen M, Nakano-Doi A, Saino O, Takata M, Nakagomi T, Luiten P, Matsuyama T.
  • Organizer: XXXth International Symposium on Cerebral Blood Flow, Metabolism and Function & Xth International Conference on Quantification of Brain Function and PET
  • Place of Presentation: Barcelona, Spain
• [Presentation] 一過性脳虚血誘導性神経幹細胞発現の発現 (2011)
  • Author(s): 百田義弘、中込隆之、中野亜紀子、田口明彦、柴田啓貴、田片将士、塚本吉胤、小谷順一郎、松山知弘
  • Organizer: 第23回日本脳循環代謝学会総会
  • Place of Presentation: 東京
• [Presentation] 大脳皮質梗塞後に誘導される脳軟膜由来神経幹細胞の発見 (2011)
  • Author(s): 中込隆之、土居亜紀子、田片将士、盧山、立花久大、田口明彦、土江伸誉、松山知弘
  • Organizer: 第23回日本脳循環代謝学会総会
  • Place of Presentation: 東京
• [Presentation] Ischemia-induced neural stem/progenitor cells express pyramidal cell-markers in vivo and in vitro (2011)
  • Author(s): Clausen M, Nakano-Doi A, Saino O, Takata M, Nakagomi T, Luiten P, Matsuyama T
  • Organizer: XXVth International Symposium on Cerebral Blood Flow, Metabolism and Function &Xth International Conference on Quantification of Brain Function and PET
  • Place of Presentation: Barcelona, Spain
• [Presentation] Effect of Soluble Cilostazol on Cerebral Blood Flow after Acute Ischemic Stroke in Mice (2011)
  • Author(s): Lu S, Nakagomi T, Nakano-Doi A, Takata M, Matsuyama T.
  • Organizer: XXVth International Symposium on Cerebral Blood Flow, Metabolism and Function & Xth International Conference on Quantification of Brain Function and PET
  • Place of Presentation: Barcelona, Spain
• [Presentation] Edaravone is effective to ameliorate the sensory neurologic deficit developed in chronic phase of cerebral infarction in mice (2011)
  • Author(s): Matsuyama T, Doe N, Nakagomi T, Nakano-Doi A, Saino O, Takata M, Lu S, Mimura O, Tachibana H, Taguchi A
  • Organizer: XXVth International Symposium on Cerebral Blood Flow, Metabolism and Function & Xth International Conference on Quantification of Brain Function and PET
  • Place of Presentation: Barcelona, Spain
• [Presentation] Expression of neural stem cells induced by transient cortical ischemia in mice (2011)
  • Author(s): Momota Y, Nakagomi T, Nakano-Doi A, Saino O, Y, Taguchi A, Takata M, Tsukamoto Y, Miyamae M, Kotani J, Matsuyama T
  • Organizer: XXVth International Symposium on Cerebral Blood Flow, Metabolism and Function & Xth International Conference on Quantification of Brain Function and PET
  • Place of Presentation: Barcelona, Spain
• [Presentation] Glucocorticoid-induced TNF receptor-stimulated T cells serve as a negative regulator for CNS repair after cerebral infarction. XXVth International Symposium on Cerebral Blood Flow, Metabolism and Function & Xth International Conference on Quantification of Brain Function and PET, Brain 2011, 5.24-28, Barcelona, Spain (2011)
  • Author(s): Takata M, Nakagomi T, Kashiwamura S, Nakano-Doi A, Saino O, Okamura H, Mimura O, Soma T, Taguchi A, Matsuyama T
  • Organizer: XXVth International Symposium on Cerebral Blood Flow, Metabolism and Function & Xth International Conference on Quantification of Brain Function and PET
  • Place of Presentation: Barcelona, Spain
• [Presentation] Bone marrow mononuclear cells improve sensory disturbance with promoting endogenous neurogenesis at the ipsilateral thalamus damaged after cortical infarction (2011)
  • Author(s): Nakano-Doi A, Doe N, Nakagomi T, Takata M, Lu S, Kanaoka S, Tachibana H, Taguchi A, Matsuyama T
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] 一過性脳虚血誘導性神経幹細胞の発現 (2011)
  • Author(s): 百田義弘, 中込隆之, 中野亜紀子, 田口明彦, 柴田啓貴, 田片将士, 塚本吉胤, 小谷順一郎, 松山知弘
  • Organizer: 第23回日本脳循環代謝学会総会
  • Place of Presentation: 東京
• [Presentation] マウス虚血再灌流モデルにおける神経幹細胞発現の検討 (2010)
  • Author(s): 百田義弘、中込隆之、中野亜紀子、沖中由佳、斉野織恵、笠原由紀子、田口明彦、田片将士、盧山、宮前雅見、小谷順一郎、松山知弘
  • Organizer: 第22回日本脳循環代謝学会総会
  • Place of Presentation: 大阪
• [Presentation] Bone marrow mononuclear cells promote proliferation of endogenous neural stem cells through vascular niches after cerebral infarction (2010)
  • Author(s): Nakano-Doi A, Nakagomi T, Nakagomi N, Saino O, Takata M, Lu S, Tachibana H, Taguchi A, Matsuyama T.
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸
• [Presentation] Effect of soluble cilostazol on cerebral blood flow after acute ischemic stroke in mice. (2010)
  • Author(s): Lu S, Nakagomi T, et al
  • Organizer: Neuro2010(第33回日本神経科学大会)
  • Place of Presentation: 神戸
• [Presentation] Depletion of CD4-positive T-lymphocytes Enhances Post-stroke Endogenous Neurogenesis. (2010)
  • Author(s): Saino O, Nakagomi T, et al
  • Organizer: Neuro2010(第33回日本神経科学大会)
  • Place of Presentation: 神戸
• [Presentation] Bone marrow mononuclear cells promote proliferation of endogenous neural stem cells through vascular niches after cerebral infarction. (2010)
  • Author(s): Nakano-Doi A, Nakagomi T, et al
  • Organizer: Neuro2010(第33回日本神経科学大会)
  • Place of Presentation: 神戸
• [Presentation] GITR (Glucocorticoid-induced TNF receptor)-positive T lymphocytes regulate endogenous neurogenesis in post-stroke brain. (2010)
  • Author(s): Takata M, Nakagomi T, et al
  • Organizer: Neuro2010(第33回日本神経科学大会)
  • Place of Presentation: 神戸
• [Presentation] 可溶化シロスタゾール脳梗塞急性期投与の脳血流に及ぼす影響の検討 (2010)
  • Author(s): 盧山, 中込隆之, 他
  • Organizer: 第22回日本脳循環代謝学会総会
  • Place of Presentation: 大阪
• [Presentation] Glucocorticoid-induced TNF receptor(GITR)刺激が脳梗塞後の神経再生に及ぼす影響 (2010)
  • Author(s): 田片将士, 中込隆之, 他
  • Organizer: 第22回日本脳循環代謝学会総会
  • Place of Presentation: 大阪
• [Presentation] Telmisartan suppresses cerebral reperfusion injury through activation of eNOS and ERK1/2 in endothelial cells. (2010)
  • Author(s): Kasahara Y, Nakagomi T, et al
  • Organizer: 7th World Stroke Congress
  • Place of Presentation: Soeal, Korea
• [Presentation] GITR (Glucocorticoid-induced TNF receptor) triggering reduces, and its inhibition enhances, endogenous neurogenesis in post-strok brain. (2010)
  • Author(s): Takata M, Nakagomi T, et al
  • Organizer: 7th World Stroke Congress
  • Place of Presentation: Soeal, Korea
• [Presentation] CD25-positive T lymphocytes are key immunomodulators of neurogenesis after stroke. (2010)
  • Author(s): Matsuyama T, Nakagomi T, et al
  • Organizer: 7th World Stroke Congress
  • Place of Presentation: Soeal, Korea
• [Presentation] A simple and highly reproducible model of permanent cerebral ischemia in CB-17 and SCID mice. (2010)
  • Author(s): Taguchi A, Nakagomi T, et al
  • Organizer: 7th World Stroke Congress
  • Place of Presentation: Soeal, Korea
• [Presentation] 骨髄単核球投与が脳梗塞後の脳血管内皮細胞と神経幹細胞に及ぼす影響の検討 (2009)
  • Author(s): 中野亜紀子、中込隆之、藤川昌敏、盧山、斎野織恵、田片将士、中込奈美、田浦映恵、田口明彦、松山知弘
  • Organizer: 第21回日本脳循環代謝学会総会
  • Place of Presentation: 大阪
• [Presentation] 実験的脳梗塞モデルにおける免疫動態の検討 (2009)
  • Author(s): 田片将士、中込隆之, 他
  • Organizer: 第21回日本脳循環代謝学会総会
  • Place of Presentation: 大阪
• [Presentation] T細胞除去が脳梗塞後の組織修復と機能回復に与える影響の検討 (2009)
  • Author(s): 斎野織恵、中込隆之, 他
  • Organizer: 21回日本脳循環代謝学会総会
  • Place of Presentation: 大阪
• [Book] Ischemia-induced neural stem/progenitor cells within the post-stroke cortex in adult brains. Neural Stem Cells and Therapy (2012)
  • Author(s): Nakagomi T and Matsuyama T.
• [Book] Ischemia-induced neural stem/progenitor cells within the post-stroke cortex in adult brains. Neural Stem Cells and Therapy, Chapter 16, Edited by : Tao Sun. ISBN 978-953-307-958-5 (2012)
  • Author(s): Nakagomi T, Matsuyama T
  • Total Pages: 440
  • Publisher: InTech-Open Access Publisher
• [Book] Neurogenesis in the cerberal cortex after stroke. Stem Cells and Cancer Stem Cells, Volume 2(M.A.Hayat (ed.)) (2011)
  • Author(s): Kasahara Y, Nakagomi T, Matsuyama T, Taguchi A
  • Total Pages: 384
  • Publisher: Springer Science+Business Media B.V.