Analysis of neural tissue development using ES cells culture system

• Project/Area Number: 21700367
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: EIRAKU Mototsugu The Institute of Physical and Chemical Research, 立体組織形成・解析ユニット, 副ユニットリーダー (40415097)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ES細胞 / 形態形成 / 器官発生 / 試験管内再構築 / 神経発生 / イメージング / 組織形成 / 幹細胞

Research Abstract

In this study, we report the dynamic, autonomous formation of the optic cup (retinal primordium) structure from a three-dimensional culture of mouse ES cell aggregates. ES cell-derived retinal epithelium spontaneously formed hemispherical epithelial vesicles that become patterned along its proximal-distal axis. While the rigid proximal portion differentiated into pigment epithelium, the flexible distal portion progressively folded inward to form a shape reminiscent of the embryonic optic cup, exhibited interkinetic nuclear migration and generated stratified neural retinal tissue, just as seen in vivo. We demonstrate that optic-cup morphogenesis in this simple cell culture depends on an intrinsic self-organizing program involving stepwise and domain-specific regulations in local epithelial properties.

Research Products

• [Journal Article] Self-organizing optic-cup morphogenesis in three-dimensional culture. (2011)
  • Author(s): Eiraku M, Takata N, Ishibashi H, Kawada M, Sakakura E, Okuda S, Sekiguchi K, Adachi T, Sasai Y
  • Journal Title: Nature 472 Pages: 51-56
  • Peer Reviewed
• [Journal Article] Subregional specification of embryonic stem cell-derived ventral telencephalic tissues by timed and combinatory treatment with extrinsic signals. (2011)
  • Author(s): Danjo T, Eiraku M, Muguruma K, Watanabe K, Kawada M, Yanagawa Y, Rubenstein JL, Sasai Y
  • Journal Title: J Neurosci. 31 Pages: 1919-1933
  • Peer Reviewed
• [Journal Article] Subregional specification of embryonic stem cell-derived ventral telencephalic tissues by timed and combinatory treatment with extrinsic signals. (2011)
  • Author(s): Danjo T, Eiraku M, Muguruma K, Watanabe K, Kawada M, Yanagawa Y, Rubenstein JL, Sasai Y.
  • Journal Title: Journal of Nuroscience Volume: 31 Pages: 1919-1933
  • Peer Reviewed
• [Journal Article] Self-organizing optic-cup morphogenesis in three-dimensional culture (2011)
  • Author(s): Eiraku M, Takata N, Ishibashi H, Kawada M, Sakakura E, Okuda S, Sekiguchi K, Adachi A, Sasai Y.
  • Journal Title: Nature Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Molecular pathway and cell state responsible for dissociation-induced apoptosis in human pluripotent stem cells. (2010)
  • Author(s): Ohgushi M, Matsumura M, Eiraku M, Murakami K, Aramaki T, Nishiyama A, Muguruma K, Nakano T, Suga H, Ueno M, Ishizaki T, Suemori H, Narumiya S, Niwa H, Sasai Y
  • Journal Title: Cell Stem Cell 7 Pages: 225-239
  • Peer Reviewed
• [Journal Article] Molecular pathway and cell state responsible for dissociation-induced apoptosis in human pluripotent stem cells. (2010)
  • Author(s): Ohgushi M, Matsumura M, Eiraku M, Murakami K, Aramaki T, Nishiyama A, Muguruma K, Nakano T, Suga H, Ueno M, Ishizaki T, Suemori H, Narumiya S, Niwa H, Sasai Y.
  • Journal Title: Cell Stem Cell Volume: 7 Pages: 225-239
  • Peer Reviewed
• [Presentation] 神経前駆細胞の細胞周期進行が脳形成に持つ意義の解明に向けて (2009)
  • Author(s): 永楽元次、笹井芳樹
  • Organizer: 第32回日本神経科学大会シンポジウム,演題「ES細胞からの大脳皮質神経組織分化について」
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-09-17
• [Presentation] ES細胞からの大脳皮質神経組織分化について (2009)
  • Author(s): 永樂元次、笹井芳樹
  • Organizer: 第32回に本神経科学大会シンポジウム
  • Place of Presentation: 名古屋国際会議場・名古屋
  • Year and Date: 2009-09-17
• [Remarks] ホームページ等
  • URL: http://www.riken.go.jp/r-world/research/lab/cdb/fdta/index.html