| Project/Area Number |
21700369
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neuroscience in general
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
MIZUGUCHI Rumiko The Institute of Physical and Chemical Research, シナプス分子機構研究チーム, 研究員 (70450418)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 神経発生 / 嗅覚神経回路 / 僧帽・房飾細胞 / T-box転写因子 / Tbx21(T-bet) / Tbr2(Eomes) / 遺伝子ターゲッティングマウス / 僧帽細胞・房飾細胞 / Tbx21 / Tbr2 |
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| Research Abstract |
To explore the functional roles of Tbx21 and Tbr2 in mitral/tufted cells in the mouse olfactory bulb, I analyzed their loss-of-function phenotypes using gene-targeting strategy. Tbx21 konckout mice showed a slight delay of regenerated olfactory sensory neurons to innervate the olfactory bulb, but the development of mitral/tufted cells was grossly normal. In contrast, loss of Tbr2 in mitral/tufted cells resulted in expressional changes of developmentally-regulated genes and abnormalities in dendritic morphology of mitral/tufted cells, which led to defects in interactions with inhibitory interneurons in the olfactory bulb. These results demonstrate that Tbr2 is required for proper differentiation of mitral/tufted cells as well as neuronal wiring in the olfactory bulb. It is also revealed that Tbx21 and Tbr21 have different functions in mitral/tufted cells.
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