Analysis of the gamma-secretase interactome and regulation mechanisms of the enzymatic activity.

Research Project

Project/Area Number	21700392
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Nerve anatomy/Neuropathology
Research Institution	The University of Tokyo
Principal Investigator	WAKABAYASHI Tomoko The University of Tokyo, 大学院・医学系研究科, 助教 (20530330)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000) Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	脳神経疾患 / アルツハイマー病
Research Abstract	In this study, we searched for γ-secretase interacting proteins using proteomic analysis, and screened for proteins that affected either enzymatic activity or subcellular localization ofγ-secretase. As a result, we identified tetraspanins and tetraspanin-associated proteins that have been known to form the lipid microdomain in the cellular membranes (called tetraspanin web or TEM). We found the possibility that tetraspanins and tetraspanin-associated proteins provide a platform for γ-secretase cleavage and regulate its enzymatic activity.

Report

(3 results)