Investigation of the mechanisms by which antibody to VZV IE62 protein augments mechanical allodynia

• Project/Area Number: 21700400
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: University of Toyama
• Principal Investigator: TAKASAKI Ichiro 富山大学, 生命科学先端研究センター, 助教 (00397176)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 帯状疱疹痛 / 帯状疱疹後神経痛 / IE62 / 帯状疱疹 / 脳由来神経栄養因子 / アロディニア / Galectin-3 / 帯状庖疹 / 帯状庖疹後神経痛

Research Abstract

Acute herpetic pain and postherpetic neuralgia are intractable neurogenic pain, however the mechanisms of the pain are still unknown. We previously found that immunoreaction to antibody to IE62, one of immediate early protein produced by varicella zoster virus, augment mechanical allodynia in mice with peripheral nerve injury via activation of BDNF activity. In this research, we found that antibody to IE62 augment the acute herpetic pain and increase the transition to postherpetic neuralgia in mice. Using global gene expression analysis, we revealed that spinal galectin-3 is involved in the acute herpetic allodynia. However, we found that galectin-3 is not involved in the anti-IE62 antibody-mediated augmentation of mechanical allodynia.

Research Products

• [Journal Article] Contribution of spinal galectin-3 to acute herpetic allodynia in mice (2012)
  • Author(s): Takasaki I, Taniguchi K, Komatsu F, Sasaki A, Andoh T, Nojima H, Shiraki K, Hsu DK, Liu FT, Kato I, Hiraga K, Kuraishi Y.
  • Journal Title: Pain Volume: 153 Pages: 585-592
  • Peer Reviewed
• [Journal Article] Antibody to Varicella-Zoster Virus Immediate Early 62 Augments Allodynia in Zoster via Brain-Derived Neurotrophic Factor (BDNF) (2010)
  • Author(s): Hama Y, Shiraki K, Yoshida Y, Maruyama A, Yasuda M, Tsuda M, Honda M, Takahashi M, Higuchi H, Takasaki I, Daikoku T, Tsumoto T
  • Journal Title: J Virol Volume: 84 Pages: 1616-1624
  • Peer Reviewed
• [Journal Article] Antibody to varicella-zoster virus immediate-early protein 62 augments allodynia in zoster via brain-derived neurotrophic factor. (2010)
  • Author(s): Hama, et al.
  • Journal Title: J Virol 84(3) Pages: 1616-1624
  • Peer Reviewed
• [Journal Article] Peripheral-type benzodiazepine receptor antagonist is effective in relieving neuropathic pain in mice. (2009)
  • Author(s): Kondo, et al.
  • Journal Title: J Pharmacol Sci 110(1) Pages: 55-63
  • Peer Reviewed
• [Presentation] 帯状疱疹痛における脊髄galectin-3の関与 (2011)
  • Author(s): 高崎一朗, 佐々木淳, 安東嗣修, 倉石泰
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] Contribution of spinal galectin-3 to acute herpetic allodynia in mice (2011)
  • Author(s): Takasaki I.
  • Organizer: The 6th International Conference of Neurons and Brain Diseases
  • Place of Presentation: Toyama
• [Presentation] 帯状庖疹痛と帯状庖疹後神経痛の動物モデルの開発及びアロディニアの発生と抑制作用の機序の解明 (2010)
  • Author(s): 高崎一朗
  • Organizer: 日本薬学会第130年会(奨励賞受賞講演)
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2010-03-28
• [Presentation] Antibody to varicella-zoster virus protein IE62 augments neuropathic allodynia via BDNF activity in mice (2010)
  • Author(s): Takasaki I., Hama Y., Yoshida Y., Daikoku T., Tsuda M., Tsumoto T., Shiraki K.
  • Organizer: The 40th Annual Meeting of Neuroscience (Neuroscience2010)
  • Place of Presentation: San Diego, USA
• [Presentation] Antibody to varicella-zoster virus immediate-early protein IE62 interacts with BDNF and enhances neuropathic allodynia (2010)
  • Author(s): Takasaki I., Hama Y., Yoshida Y., Daikoku T., Tsuda M., Tsumoto T., Shiraki K.
  • Organizer: 第33回日本神経科学学会
  • Place of Presentation: 神戸
• [Presentation] 帯状疱疹痛と帯状疱疹後神経痛の動物モデルの開発及びアロディニアの発生と抑制作用の機序の解明 (2010)
  • Author(s): 高崎一朗
  • Organizer: 日本薬学会第139年会
  • Place of Presentation: 岡山
• [Presentation] Antibody to varicella-zoster virus protein IE62 augments neuropathic allodynia via BDNF activity in mice (2010)
  • Author(s): Takasaki I., et al
  • Organizer: Neuroscience 2010
  • Place of Presentation: San Diego, CA, USA