Analysis plasticity of monoaminergic and cholinergic neurons in nalcoleptic mice

• Project/Area Number: 21700401
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Kanazawa University
• Principal Investigator: TSUJINO Natsuko 金沢大学, 医学系, 助教 (40432166)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 神経疾患の病態と治療 / 神経ペプチド / 睡眠障害 / 神経疾患 / オレキシン / ノルアドレナリン / ナルコレプシー / モノアミン / 睡眠 / 神経変性疾患 / パッチクランプ / ノルアジレナリン神経

Research Abstract

I examined monoaminergic neuronal activities of narcoleptic mice (orexin neuron deficiency mice). Firing frequency of Locus ceruleus noradrenergic neuron was increased in narcoleptic mice by in vivo extracellular recording during wakefulness and slow wave sleep. In vitro patch clamp recording, IPSC of noradrenergic neuron was reduced in narcoleptic mice. This reduction might be results the increasing of firing frequency of noradrenergic neuron in narcoleptic mice.

Research Products

• [Journal Article] Pharmacogenetic modulation of orexin neurons alters sleep/wakefulness states in mice (2011)
  • Author(s): Sasaki K, Suzuki M, Mieda M, Tsujino N, Roth B, Sakurai T
  • Journal Title: PLoS One Volume: 6(5)
  • NAID: 120003141898
  • Peer Reviewed
• [Journal Article] Critical role of neuropeptides b/w receptor 1 signaling in social behavior and fear memory (2011)
  • Author(s): Nagata-Kuroiwa R, Furutani N, Hara J, Hondo M, Ishii M, Abe T, Mieda M, Tsujino N, Motoike T, Yanagawa Y, Kuwaki T, YamamotoM, Yanagisawa M, Sakurai T
  • Journal Title: PLoS One Volume: 6(2)
  • NAID: 120007130926
  • Peer Reviewed
• [Journal Article] Thrombin activates Ca^<2+>-permiating nonselective cation channels through protein kinase C in human umbilical vein endothelial cells (2011)
  • Author(s): Tsujino N, Nakada T, Tsubouchi K, Kobayashi M, Kawai Y, Yano S, Matsunaga T, Hirose M, Ohmori S, Ohhashi T, Yamada M
  • Journal Title: Shinshu Medical Journal Volume: 59(1) Pages: 13-26
  • Peer Reviewed
• [Journal Article] Nicorandil ameliorates impulse conduction disturbances during ischemia in isolated arterially perfused canine atria (2011)
  • Author(s): Hirose M, Yano S, Nakada T, Horiuchi-Hirose M, Tsujino N, Yamada M
  • Journal Title: nternational Journal of Cardiology Volume: 146(1) Pages: 37-43
  • Peer Reviewed
• [Journal Article] Critical role of neuropeptides b/w receptor 1 signaling in social behavior and fear memory (2011)
  • Author(s): Nagata-Kuroiwa R, Furutani N, Hara J, Hondo M, Ishii M, Abe T, Mieda M, Tsujino N, Motoike T, Yanagawa Y, Kuwaki T, Yamamoto M, Yanagisawa M, Sakurai T
  • Journal Title: PLoS One Volume: 6(2)
  • NAID: 120007130926
  • Peer Reviewed
• [Journal Article] Nicorandil ameliorates impulse conduction disturbances during ischemia in isolated arterially perfused canine atria (2011)
  • Author(s): Hirose M, Yano S, Nakada T, Horiuchi-Hirose M, Tsujino N, Yamada M
  • Journal Title: International Journal of Cardiology Volume: 146(1) Pages: 37-43
  • Peer Reviewed
• [Journal Article] Activation of Bombesin Receptor Subtype-3 Influences Activity of Orexin Neurons by Both Direct and Indirect Pathways (2010)
  • Author(s): Furutani N, Hondo M, Tsujino N, Sakurai T
  • Journal Title: Journal of Molecular Neuroscience Volume: 42(1) Pages: 106-11
  • NAID: 120002384286
  • Peer Reviewed
• [Journal Article] The orexin system: Roles in sleep/wake regulation (2010)
  • Author(s): Sakurai, T., Mieda, M., Tsujino, N.
  • Journal Title: Annals of the New York Academy of Sciences Volume: 1200 Pages: 149-161
  • NAID: 120002384291
  • Peer Reviewed
• [Journal Article] The orexin system : Roles in sleep/wake regulation (2010)
  • Author(s): Sakurai, T., Mieda, M., Tsujino, N.
  • Journal Title: Annals of the New York Academy of Sciences Volume: 1200 Pages: 149-161
  • NAID: 120002384291
  • Peer Reviewed
• [Journal Article] Orexin/Hypocretin: a neuropeptide at the interface of sleep, energy homeostasis, and reward system (2009)
  • Author(s): Tsujino N, Sakurai T
  • Journal Title: Pharmacological Reviews Volume: 61(2) Pages: 162-76
  • Peer Reviewed
• [Journal Article] Nicorandil ameliorates impulse conduction disturbances during ischemia in isolated arterially perfused canine atria. (2009)
  • Author(s): Hirose M, Yano S, Nakada T, Horiuchi-Hirose M, Tsujino N, Yamada M
  • Journal Title: International journal of cardiology
  • Peer Reviewed
• [Journal Article] Orexin/Hypocretin : a neuropeptide at the interface of sleep, energy homeostasis, and reward system. (2009)
  • Author(s): Tsujino N, Sakurai T
  • Journal Title: Pharmacological reviews 61 Pages: 162-179
  • Peer Reviewed
• [Presentation] Chronic functional alterations of locus ceruleus noradrenergic neurons in orexin neuron-ablated narcoleptic mice (2012)
  • Author(s): 辻野なつ子、常松友美、山中章弘、渡辺雅彦、小山純正、桜井武
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 信州大学(長野県)
  • Year and Date: 2012-03-31
• [Presentation] Chronic functional alterations of locus ceruleus noradrenergic neurons in orexin neuron-ablated narcoleptic mice (2011)
  • Author(s): 辻野なつ子、常松友美、山中章弘、小山純正、桜井武
  • Organizer: Worldsleep 2011
  • Place of Presentation: 京都国際会館(京都府)
  • Year and Date: 2011-10-19
• [Presentation] Chronic functional alterations of locus ceruleus noradrenergic neurons in orexin neuron-ablated narcoleptic mice (2011)
  • Author(s): 辻野なつ子、常松友美、山中章弘、小山純正、桜井武
  • Organizer: Worldsleep 2011
  • Place of Presentation: 国際会館(京都府)(招待講演)
  • Year and Date: 2011-10-19
• [Presentation] Alterations in noradrenergic neurons in orexin neuron-deficient mice (2011)
  • Author(s): 辻野なつ子、常松友美、山中章弘、小山純正、桜井武
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-15
• [Presentation] Functional and histological interaction between preoptic area GABAergic neurons and orexin neurons (2011)
  • Author(s): 辻野なつ子、齊藤夕貴、本堂茉莉、三枝理博、桜井武
  • Organizer: 第88回日本生理学会大会
  • Place of Presentation: 学会誌上開催
• [Presentation] Functional and histological interaction between preoptic area GABAergic neurons and orexin neurons (2011)
  • Author(s): 辻野なつ子、齊藤夕貴、本堂茉莉、三枝理博、桜井武
  • Organizer: 第88回日本生理学会大会
  • Place of Presentation: (学会誌上開催)
• [Presentation] Alterations in noradrenergic neurons in orexin neuron-ablated mice (2010)
  • Author(s): 辻野なつ子、常松友美、山中章弘、小山純正、桜井武
  • Organizer: 第87回日本生理学会
  • Place of Presentation: 岩手市民文化ホール(岩手県)
  • Year and Date: 2010-05-19
• [Presentation] Alterations in noradrenergic neurons in orexin neuron-ablated mice (2010)
  • Author(s): 辻野なつ子、常松友美、山中章弘、小山純正、桜井武
  • Organizer: 第87回日本生理学会
  • Place of Presentation: 盛岡市民文化ホール(岩手県)
  • Year and Date: 2010-05-19
• [Presentation] Regulation of orexin neurons by bombesin receptor subtype 3 (2010)
  • Author(s): 辻野なつ子、古谷直生、本堂茉莉、桜井武
  • Organizer: Official Satellite Symposium of the 14^<th> International Congress of Endocrinology (ICE2010) : Obesity and Metabolic Syndrome
  • Place of Presentation: 京都大学芝蘭会館(京都府)
  • Year and Date: 2010-03-30
• [Presentation] オレキシン神経欠損マウスでのノルアドレナリン作動性神経活動の解析 (2009)
  • Author(s): 辻野なつ子、常松友美、山中章弘、小山純正、桜井武
  • Organizer: 第56回中部日本生理学会
  • Place of Presentation: 音楽堂(石川県)
  • Year and Date: 2009-12-04
• [Presentation] オレキシン神経欠損マウスでのノルアドレナリン作動性神経活動の解析 (2009)
  • Author(s): 辻野なつ子、常松友美、山中章弘、小山純正、桜井武
  • Organizer: 第56回中部日本生理学会
  • Place of Presentation: 県立音楽堂 石川
  • Year and Date: 2009-12-04
• [Presentation] Alterations in noradrenergic neurons in the narcoleptic mice (2009)
  • Author(s): 辻野なつ子、常松友美、小山純正、山中章弘、桜井武
  • Organizer: 第9回VIP/PACAP関連ペプチド国際シンポジウム サテライトシンポジウム
  • Place of Presentation: いわさきホテル(鹿児島県)
  • Year and Date: 2009-10-02
• [Presentation] オレキシン神経欠損マウスにおけるモノアミン神経活動の変化 (2009)
  • Author(s): 辻野なつ子、常松友美、山中章弘、小山純正、桜井武
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 国際会議場(愛知県)
  • Year and Date: 2009-09-18
• [Presentation] オレキシン神経欠損マウスにおけるモノアミン神経活動の変化 (2009)
  • Author(s): 辻野なつ子、常松友美、山中章弘、小山純正、桜井武
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場 愛知
  • Year and Date: 2009-09-18
• [Presentation] Alterations in monoaminergic neurons in orexin neuron-ablated mice (2009)
  • Author(s): 辻野なつ子、常松友美、山中章弘、小山純正、桜井武
  • Organizer: 第36回国際生理学会世界大会
  • Place of Presentation: 国際会館(京都)
  • Year and Date: 2009-08-01
• [Presentation] Alterations in monoaminergic neurons in orexin neuron-ablatedmice. (2009)
  • Author(s): 辻野なつ子、常松友美、山中章弘、小山純正、桜井武
  • Organizer: 第36回 国際生理学会世界大会
  • Place of Presentation: 京都国際会館 京都
  • Year and Date: 2009-08-01