Molecular characterization of a novel ER stress inducible factor, MANF

• Project/Area Number: 21700403
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Gifu University
• Principal Investigator: OH-HASHI Kentaro Gifu University, 工学部, 助教 (50332953)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 小胞体ストレス / ATF6 / MANF / CRELD2 / 神経栄養因子 / 神経変性疾患

Research Abstract

Using microarray analysis and RT-PCR, we found that two types of MANF mRNA up-regulation by treatment with thapsigargin, an ER stress inducer. Afer constructing various expression plasmids of mouse MANF mutants in addition to two full-length mouse MANF, we evaluated the effects of MANF-overexpression on stress-induced cell death and examined the processing and secretion of wild type and mutated MANF.

Research Products

• [Journal Article] Role of an ER stress response element in regulating the bidirectional promoter of the mouse CRELD2-ALG12 gene pair. (2010)
  • Author(s): Oh-hashi K, Koga H, Ikeda S, Shimada K, Hirata Y, Kiuchi K.
  • Journal Title: BMC Genomics. 11 Pages: 664-664
• [Journal Article] Role of an ER stress response element in regulating the bidirectional promoter of the mouse CRELD2-ALG12 gene pair. (2010)
  • Author(s): Oh-hashi K, et al.
  • Journal Title: BMC Genomics. Volume: 11 Pages: 664-664
  • Peer Reviewed
• [Journal Article] CRELD2 is a novel endoplasmic reticulum stress-inducible gene. (2009)
  • Author(s): Oh-hashi K, Koga H, Ikeda S, Shimada K, Hirata Y, Kiuchi K.
  • Journal Title: Biochem.Biophys.Res.Commun. 387(3) Pages: 504-510
• [Journal Article] CRELD2 is a novel endoplasmic reticulum stress-inducible gene (2009)
  • Author(s): Oh-hashi K, et al., (他5名)
  • Journal Title: Biochem Biophys Res Commun 387 Pages: 504-510
  • Peer Reviewed
• [Presentation] 新規小胞体ストレス誘導因子CRELD2の細胞内挙動の解析 (2010)
  • Author(s): 国枝亮佑、大橋憲太郎、平田洋子、木内一壽
  • Organizer: 第33回日本分子生物学会・第83回日本生化学大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] 新規小胞体ストレス誘導遺伝子CRELD2の発現制御機構の解析 (2010)
  • Author(s): 大橋憲太郎、平田洋子、木内一壽
  • Organizer: 第33回日本基礎老化学会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-06-17
• [Presentation] 新規小胞体ストレス誘導遺伝子CRELD2の発現制御機構の解析 (2010)
  • Author(s): 大橋憲太郎, et al.
  • Organizer: 第33回日本基礎老化学会
  • Place of Presentation: 名古屋大学
• [Remarks] ホームページ
• [Remarks] 岐阜大学工学部生命工学科生命情報工学講I
  • URL: http://www1.gifu-u.ac.jp/~bioinfo1/Bioinfo1/BIoinfo1.html
• [Remarks] 岐阜大学工学部生命工学科生命情報工学講I
  • URL: http://www1.gifu-u.ac.jp/~bioinfo1/Bioinfo1/BIoinfo1.html