A light induced loss of function technology for AMPA receptors

• Project/Area Number: 21700412
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Yokohama City University
• Principal Investigator: TAKEMOTO Kiwamu Yokohama City University, 医学部, 助教 (80466432)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 光操作 / イメージング / 生体操作

Research Abstract

Neurons communicate with each other by electronic signals through synapses to exhibit the brain function. Function of each synapse was not identified in learning and memory, however, technology of brain decoding at synaptic resolution was not exist. In this research, we developed a light induced loss of function technology for AMPA receptors that is important for synaptic function.

Research Products

• [Journal Article] Chromophore-assisted light inactivation of HaloTag fusion proteins labeled with eosin in living cells (2011)
  • Author(s): Takemoto K, Matsuda T, McDougall M, Klaubert D, Hasegawa A, Los G, Wood K,Miyawaki A, Nagai T
  • Journal Title: ACS.Chem.Biol. 印刷中
  • Peer Reviewed
• [Journal Article] Serotonin mediates cross-modal reorganization of cortical circuits. (2011)
  • Author(s): Jitsuki S, Takemoto K, Kawasaki T, Tada T, Takahashi A, Becamel C, Sano A, Yuzaki M, Zukin RS, Ziff EB, Kessels HW, Takahashi T.
  • Journal Title: Neuron 69巻 Pages: 780-792
  • Peer Reviewed
• [Journal Article] Apoptosis controls the speed of looping morphogenesis in Drosophila male terminalia (2011)
  • Author(s): Kuranaga E, Matsunuma T, Kanuka H, Takemoto K, Koto A, Kimura K, Miura M
  • Journal Title: Development 138巻 Pages: 1493-1499
  • Peer Reviewed
• [Journal Article] Chromophore-assisted light inactivation of Halo Tag fusion proteins labeled with eosin in living cells (2011)
  • Author(s): Takemoto K, Matsuda T, McDougall M, Klaubert D, Hasegawa A, Los G, Wood K, Miyawaki A, Nagai T
  • Journal Title: ACS Chem.Biol. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Serotonin mediates cross-modal reorganization of cortical circuits. (2011)
  • Author(s): Jitsuki S, Takemoto K, Kawasaki T, Tada T, Takahashi A, Becamel C, Sano A, Yuzaki M, Zukin RS, Ziff EB, Kessels HW, Takahashi T.
  • Journal Title: Neuron Volume: 69 Pages: 780-792
  • Peer Reviewed
• [Journal Article] Apoptosis controls the speed of looping morphogenesis in Drosophila male terminalia (2011)
  • Author(s): Kuranaga E, Matsunuma T, Kanuka H, Takemoto K, Koto A, Kimura K, Miura M
  • Journal Title: Development Volume: 138 Pages: 1493-1499
  • Peer Reviewed
• [Presentation] Light induced loss of function technology for AMPA receptors in living cells (2010)
  • Author(s): Kiwamu Takemoto, Takeharu Nagai, Takuya Takahashi
  • Organizer: Neuro2010
  • Place of Presentation: 神戸 神戸国際展示場1号館
  • Year and Date: 2010-09-03
• [Remarks] ホームページ等
  • URL: http://neurosci.med.yokohama-cu.ac.jp/2.html