| Project/Area Number |
21700419
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
TANAKA Kenji National Institute for Physiological Sciences, 分子生理研究系, 助教 (30329700)
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| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | グリア細胞 / アストロサイト / オリゴデンドロサイト / MLC / Mlc1 / 髄鞘 / 遺伝子改変 / 脳白質 / 脳梁 / 髄鞘形成 / 多機能遺伝子改変 |
|---|
| Research Abstract |
I succeeded to establish the animal model for a white matter disease named megalencephalic leukoencephalopathy with subcortical cysts (MLC), which is caused by MLC1 mutation. I used a versatile new gene modulation system to generate both mouse Mlc1 gene conditional knockout and overexpression. I found that Mlc1 was exclusively expressed by astrocytes and its overexpression resulted in astrocytic swelling and vacuolating myelopathy, whereas Mlc1 gene knockout mice were grossly normal.
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