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Analysis of the novel sialylated N-glycan expressed in the mouse brain

Research Project

Project/Area Number 21700420
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Neurochemistry/Neuropharmacology
Research InstitutionNational Institute for Physiological Sciences

Principal Investigator

YOSHIMURA Takeshi  生理学研究所, 分子生理研究系, 特任助教 (60402567)

Research Collaborator FUKASE Koichi  大阪大学, 大学院・理学研究科, 教授 (80192722)
TANAKA Katsunori  大阪大学, 大学院・理学研究科, 助教 (00403098)
KITAJIMA Ken  名古屋大学, 生物機能開発利用研究センター, 教授 (80192558)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords糖鎖 / シアル酸 / 神経細胞 / 糖蛋白質 / シグレック
Research Abstract

Analyses of glycosyltransferase knockout mice and congenital disorders of glycosylation reveal that N-glycans play important roles in the brain. In this study, we developed a simple method to purify and detect trace amounts of N-glycans. We focused on the analysis of the novel sialylated N-glycan structure, 6-Sialyl-Lewis^c(Galβ1, 3[NeuAcα2, 6] GlcNAc-), found in the mouse brain. We aimed to identify its interacting molecules as well as the protein on which it is beared.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (22 results)

All 2012 2011 2010 2009 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (16 results) Remarks (4 results)

  • [Journal Article] Detection of N-glycans on small amounts of glycoproteins in tissue samples and sodium dodecyl sulfate-polyacrylamide gels2012

    • Author(s)
      Yoshimura T, Yamada G, Narumi M, Koike T, Ishii A, Sela I, Mitrani-Rosenbaum S, Ikenaka K
    • Journal Title

      Analytical Biochemistry

      Volume: 423 Issue: 2 Pages: 253-260

    • DOI

      10.1016/j.ab.2012.01.023

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 脳神経の発達とN結合型糖鎖2011

    • Author(s)
      池中一裕、鳥居知宏、吉村武
    • Journal Title

      生化学

      Volume: 第83巻 Pages: 212-215

    • NAID

      10029067782

    • URL

      http://www.jbsoc.or.jp/event/magazine/index.html

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Structure determination of N-glycans on a few pmol glycoprotein and its application to the structural analysis of N-glycans on P0.ISN-ESN-20112011

    • Author(s)
      Yoshimura T
    • Organizer
      23rd Biennial Meeting
    • Place of Presentation
      Athens, Greece
    • Year and Date
      2011-08-30
    • Related Report
      2011 Final Research Report
  • [Presentation] Structure determination of N-glycans on a few pmol glycoprotein and its application to the structural analysis of N-glycans on myelin protein zero2011

    • Author(s)
      吉村武
    • Organizer
      日本生化学会中部支部会例会・シンポジウム
    • Place of Presentation
      静岡県立大学看護学部棟(静岡県)
    • Year and Date
      2011-05-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Structure determination of N-glycans on a few pmol glycoprotein and its application to the structural analysis of N-glycans on myelin protein zero2011

    • Author(s)
      Yoshimura T
    • Organizer
      THE 31st NAITO CONFERENCE, Glycan Expression and Regulation[II]: Metabolites, Stress Response, Microdomains, and Beyond
    • Place of Presentation
      Chateraise Gateaux Kingdom Sapporo, Hokkaido, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Analysis of N-glycan structures on myelin protein zero2011

    • Author(s)
      Yoshimura T
    • Organizer
      10th Biennial ISN Satellite Meeting on Myelin Biology
    • Place of Presentation
      Kolymvari, Crete, Greece
    • Related Report
      2011 Final Research Report
  • [Presentation] 末梢神経髄鞘の主要構成蛋白質myelin protein zeroの糖鎖構造2011

    • Author(s)
      吉村武
    • Organizer
      第30回日本糖質学会年会
    • Place of Presentation
      ハイブ長岡(新潟県)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Analysis of N-glycan structures on myelin protein zero2011

    • Author(s)
      Takeshi Yoshimura
    • Organizer
      10th Biennial ISN Satellite Meeting on Myelin Biology
    • Place of Presentation
      Kolymvari (Crete, Greece)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Structure determination of N-glycans on a few pmol glycoprotein and its application to the structural analysis of N-glycans on P02011

    • Author(s)
      Takeshi Yoshimura
    • Organizer
      ISN-ESN-2011, 23rd Biennial Meeting
    • Place of Presentation
      Megaron Athens International Conference Centre (Athens, Greece)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Structure determination of N-glycans on a few pmol glycoprotein and its application to the structural analysis of N-glycans on myelin protein zero2011

    • Author(s)
      Takeshi Yoshimura
    • Organizer
      THE 31st NAITO CONFERENCE, Glycan Expression and Regulation[II] : Metabolites, Stress Response, Microdomains, and Beyond
    • Place of Presentation
      Chateraise Gateaux Kingdom Sapporo (Hokkaido, Japan)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 脳発達におけるフコシル基転移酵素FUT10の役割2010

    • Author(s)
      吉村武
    • Organizer
      糖鎖科学名古屋拠点 第8回「若手の力」フォーラムおよび名古屋拠点若手研究奨励賞講演
    • Place of Presentation
      名古屋大学・VBLホール(愛知県)
    • Year and Date
      2010-09-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] 早期の肝癌を検出する新規バイオマーカー2010

    • Author(s)
      池中一裕、吉村武、東幹人、鳥居知宏
    • Organizer
      第一回 名古屋大学医学系研究科 生理学研究所 合同シンポジウム
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県)
    • Year and Date
      2010-08-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Identification and functional analysis of a novel LewisX-synthesizing α1,3-fucosyltransferase gene in neural precursor2010

    • Author(s)
      吉村武
    • Organizer
      日本生化学会中部支部会 例会・シンポジウム
    • Place of Presentation
      名古屋大学医学部(愛知県)
    • Year and Date
      2010-05-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] Analysis of N-glycans in myelin2009

    • Author(s)
      Yoshimura T
    • Organizer
      22nd Biennial Meeting of the ISN/APSN Joint Meeting
    • Place of Presentation
      BEXCO, Busan, South Korea
    • Year and Date
      2009-08-25
    • Related Report
      2011 Final Research Report
  • [Presentation] Analysis of N-glycans in myelin2009

    • Author(s)
      吉村武、Jan Sedzik、池中一裕
    • Organizer
      22nd Biennial Meeting of the ISN/APSN Joint Meeting
    • Place of Presentation
      Busan(Korea)
    • Year and Date
      2009-08-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] Analysis of N-glycan structure from a femtomole level of glycoprotein2009

    • Author(s)
      Yoshimura T
    • Organizer
      Ninth Biennial Satellite Meeting of the ISN on Myelin Biology
    • Place of Presentation
      Gyeongju, South Korea
    • Related Report
      2011 Final Research Report
  • [Presentation] Analysis of N-glycan structure from a femtomole level of glycoprotein2009

    • Author(s)
      吉村武
    • Organizer
      第52回日本神経化学会(伊香保)大会
    • Place of Presentation
      伊香保温泉ホテル天坊(群馬県)
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] Analysis of N-glycan structure from a femtomole level of glycoprotein2009

    • Author(s)
      吉村武
    • Organizer
      Ninth Biennial Satellite Meeting of the ISN on Myelin Biology
    • Place of Presentation
      Gyeongju(Korea)
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.nips.ac.jp/ninfo/index.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.nips.ac.jp/ninfo/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.nips.ac.jp/ninfo/index.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.nips.ac.jp/ninfo/index.html

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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