Epigenetic analysis in the individual level by mice engineered using artificial chromosome vectors

• Project/Area Number: 21700451
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory animal science
• Research Institution: Tottori University
• Principal Investigator: KASHIWAGI Akiko 鳥取大学, 医学部, 助教 (90335521)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: エピジェネティクス / 人工染色体 / マウス / 遺伝子改変マウス / 染色体工学 / トランスクロモソミックマウス

Research Abstract

Recently, the relevance between epigenetics and human development and diseases has been revealed. It is important to utilize model animals maintained stably the genetical modification to be verified further these findings in individual level. In this study, we succeeded the development of the novel basic technology to generate model mice maintained stably artificial chromosome vectors to monitor the variations of epigenetics in individual level. These mice models will be useful for analyses of changes of the epigenetics and physiological gene expression arising from various biological phenomena.