| Project/Area Number |
21700454
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
MAKINO Shigeru The Institute of Physical and Chemical Research, 新規変異マウス研究開発チーム, 開発研究員 (30462732)
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| Research Collaborator |
GONDO Yoichi 独立行政法人理化学研究所, 新規変異マウス研究開発チーム, チームリーダー
HUI Chi-Chung University of Toronto, The Hospital for Sick Children, Professor
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 点突然変異マウス / ENUミュータジェネシス / ヘッジホグシグナル伝達 / 発がん / Sufu / Gli3 / 形態形成 / Gene-driven mutagenesis / Gli / タンパク質の安定性 / ENU / gene-driven mutagenesis / 点突然変異 / 発生 / 神経管形成 |
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| Research Abstract |
Hedgehog (Hh) signal transduction is known to have important roles in cancer formation. To understand the in vivo function of Sufu, which is a negative mediator of Hh signaling, we made use of Sufu mutant mice, which we established by the RIKEN ENU-based gene-driven mutagenesis system. The results showed that Sufu was necessary to regulate subcellular localization as well as stabilization of the Gli3^<REP> transcription factor. These results are not consistent with the previous publications, indicating novel mechanism of Hh signal transduction.
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