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Cell-cell interaction in 3D cell culture using a original biochip

Research Project

Project/Area Number 21700458
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Biomedical engineering/Biological material science
Research InstitutionNagoya University (2010)
Tohoku University (2009)

Principal Investigator

MASUDA Taisuke  Nagoya University, 工学研究科, 特任助教 (30431513)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsバイオメカニクス / 3次元培養 / 細胞相互作用 / 3次元細胞凝集体 / マスクレス露光 / グレースケール露光 / 熱ショックタンパク質 / マイクロ流路
Research Abstract

In this study, I have paid attention to cell-cell interaction, developed an original biochip. Moreover, the cell-cell interaction was examined by using this biochip. And, it aimed at the restructuring of three-dimensional tissue. As a result, it was shown that the effect of the differentiation promotion of chondorocyte to method of three-dimensional culture proposed in the present study, and nearing to a more physiological environment.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (36 results)

All 2011 2010 2009 Other

All Journal Article (15 results) (of which Peer Reviewed: 15 results) Presentation (18 results) Remarks (3 results)

  • [Journal Article] On-Chip Magnetically Actuated Robot with Ultrasonic Vibration for Single Cell Manipulations2011

    • Author(s)
      H Hagiwara, T.Kawahara, T.Masuda, et al
    • Journal Title

      Lab on a Chip 印刷中

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nanomanipilation of single influenza virus using dielectrophoretic concentration and optical tweezers for single virus infection to a specific cell on a microfluidic chip2011

    • Author(s)
      H Maruyama, K Kotani, T.Masuda, et al
    • Journal Title

      Microfluidic Nanofluidic 10

      Pages: 1109-1117

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] On-chip magnetically actuated robot with ultrasonic vibration for single cell manipulations2011

    • Author(s)
      Masaya Hagiwara
    • Journal Title

      Lab on a Chip

      Volume: (印刷中 未定)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nanomanipilation of single influenza virus using dielectrophoretic concentration and optical tweezers for single virus infection to a specific cell on a microfluidic chip2011

    • Author(s)
      Hisataka Maruyama
    • Journal Title

      Microfluidics and Nanofluidics

      Volume: 10 Pages: 1109-1117

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of physicochemical properties of octacalcium phosphate prepared by hydrolysis and co-precipitation with fluoride ions2010

    • Author(s)
      Y Shiwaku, Y Honda, T Masuda, et al
    • Journal Title

      J Ceram Soc Japan 118

      Pages: 402-405

    • NAID

      130000304756

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quality of regenerated bone enhanced by implantation of octacalcium phosphate-collagen composite2010

    • Author(s)
      T Masuda, T Kawai, T Anada, et al
    • Journal Title

      Tissue Eng Part C 16

      Pages: 471-478

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional cell culture device utilizing thin membrane deformation by decompression2010

    • Author(s)
      T Anada, T.Masuda, Y.Honda, et al
    • Journal Title

      Sens Actuators B Chem 147

      Pages: 376-379

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Preparation and characterization of porous alginate scaffolds containing various amounts of octacalcium phosphate (OCP) crystals2010

    • Author(s)
      N Shiraishi, T Anada, T Masuda, et al
    • Journal Title

      J Mater Sci Mater Med 21

      Pages: 907-914

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of physicochemical properties of octacalcium phosphate prepared by hydrolysis and co-precipitation with fluoride ions2010

    • Author(s)
      Yukari Shiwaku
    • Journal Title

      Journal of the Ceramic Society of Japan

      Volume: 118 Pages: 402-405

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quality of regenerated bone enhanced by implantation of octacalcium phosphate-collagen composite2010

    • Author(s)
      Taisuke MASUDA
    • Journal Title

      Tissue Engineering C

      Volume: 16 Pages: 471-478

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional cell culture device utilizing thin membrane deformation by decomression2010

    • Author(s)
      Takahisa Anada
    • Journal Title

      Sensors and Actuators B

      Volume: 147 Pages: 376-379

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation and characterization of porous alginate scaffolds containing various amounts of octacalcium phosphate (OCP) crystals2010

    • Author(s)
      Naru Shiraishi
    • Journal Title

      Journal of Materials Science : Materials in Medicine

      Volume: 21 Pages: 907-914

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quality of regenerated bone enhanced by implantation of octacalcium phosphate-collagen composite2010

    • Author(s)
      Taisuke MASUDA
    • Journal Title

      Tissue Engineering C (未定, 掲載確定)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional cell culture device utilizing thin membrane deformation by decompression2010

    • Author(s)
      Takahisa ANADA
    • Journal Title

      Sensors and Actuators B (未定, 掲載確定)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Mechanisms of mechanical Stress Response during Chondrogenesis2009

    • Author(s)
      I Takahashi, T Masuda, K Kohsaka, et al
    • Journal Title

      Journal of Boimechanical Science and Engineerng, J Biomechanical Science and Engineering 4

      Pages: 307-316

    • NAID

      130000129002

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] Active virus filter for enrichment and manipulation of virus2011

    • Author(s)
      Taisuke MASUDA
    • Organizer
      24th IEEE International Conference on Micro Electro Mechanical Systems
    • Place of Presentation
      カンクン(メキシコ)
    • Year and Date
      2011-01-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] Active virus filter for enrichment and manipulation of virus2011

    • Author(s)
      T Masuda, et al
    • Organizer
      24th IEEE International Conference on Micro Electro Mechanical Systems
    • Place of Presentation
      Cancun,メキシコ
    • Year and Date
      2011-01-24
    • Related Report
      2010 Final Research Report
  • [Presentation] 細胞-細胞間微細操作における力学刺激応答2011

    • Author(s)
      益田泰輔, ほか
    • Organizer
      日本機械学会第23回バイオエンジニアリング講演会
    • Place of Presentation
      熊本
    • Year and Date
      2011-01-08
    • Related Report
      2010 Final Research Report
  • [Presentation] 単一ウイルス分離操作のための能動ウイルスフィルタ2011

    • Author(s)
      益田泰輔, ほか
    • Organizer
      日本機械学会第23回バイオエンジニアリング講演会
    • Place of Presentation
      熊本
    • Year and Date
      2011-01-08
    • Related Report
      2010 Final Research Report
  • [Presentation] MEMS技術による3次元細胞凝集体の作成2011

    • Author(s)
      益田泰輔, ほか
    • Organizer
      日本機械学会第23回バイオエンジニアリング講演会
    • Place of Presentation
      熊本
    • Year and Date
      2011-01-08
    • Related Report
      2010 Final Research Report
  • [Presentation] 細胞-細胞間微細操作における力学刺激応答2011

    • Author(s)
      益田泰輔
    • Organizer
      日本機械学会第23回バイオエンジニアリング講演会
    • Place of Presentation
      熊本大学(熊本市)
    • Year and Date
      2011-01-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 単一ウイルス分離操作のための能動ウイルスフィルタ2011

    • Author(s)
      益田泰輔
    • Organizer
      日本機械学会第23回バイオエンジニアリング講演会
    • Place of Presentation
      熊本大学(熊本市)
    • Year and Date
      2011-01-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] MEMS技術による3次元細胞凝集体の作成2011

    • Author(s)
      益田泰輔
    • Organizer
      日本機械学会第23回バイオエンジニアリング講演会
    • Place of Presentation
      熊本大学(熊本市)
    • Year and Date
      2011-01-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 能動ウイルスフィルタを用いた単一ウイルス操作2010

    • Author(s)
      益田泰輔, ほか
    • Organizer
      第11回計測自動制御学会システムインテグレーション部門講演会
    • Place of Presentation
      仙台
    • Year and Date
      2010-12-25
    • Related Report
      2010 Final Research Report
  • [Presentation] 能動ウイルスフィルタを用いた単一ウイルス操作2010

    • Author(s)
      益田泰輔
    • Organizer
      第11回計測自動制御学会システムインテグレーション部門講演会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2010-12-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] 能動ウイルスフィルタによるウイルス濃度制御2010

    • Author(s)
      益田泰輔, ほか
    • Organizer
      第22回化学とマイクロ・ナノシステム研究会
    • Place of Presentation
      名古屋
    • Year and Date
      2010-11-18
    • Related Report
      2010 Final Research Report
  • [Presentation] マスクレス・グレースケール露光を利用した3次元培養装置の作成2010

    • Author(s)
      益田泰輔, ほか
    • Organizer
      第22回化学とマイクロ・ナノシステム研究会
    • Place of Presentation
      名古屋
    • Year and Date
      2010-11-18
    • Related Report
      2010 Final Research Report
  • [Presentation] 能動ウイルスフィルタによるウイルス濃度制御2010

    • Author(s)
      益田泰輔
    • Organizer
      第22回化学とマイクロ・ナノシステム研究会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Year and Date
      2010-11-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] マスクレス・グレースケール露光を利用した3次元培養装置の作成2010

    • Author(s)
      益田泰輔
    • Organizer
      第22回化学とマイクロ・ナノシステム研究会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Year and Date
      2010-11-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] 長期培養のための3次元細胞凝集体形成デバイスの開発2010

    • Author(s)
      益田泰輔, ほか
    • Organizer
      日第28回日本ロボット学会学術講演会
    • Place of Presentation
      名古屋
    • Year and Date
      2010-09-22
    • Related Report
      2010 Final Research Report
  • [Presentation] 長期培養のための3次元細胞凝集体形成デバイスの開発2010

    • Author(s)
      益田泰輔
    • Organizer
      第28回日本ロボット学会学術講演会
    • Place of Presentation
      名古屋工業大学(名古屋市)
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] Development of a pH indicator immobilized gel-sheet for microenvironment analysis2009

    • Author(s)
      T Masuda, et al
    • Organizer
      20th IEEE International Symposium on Micro-Nano Mechatronics and Human Science
    • Place of Presentation
      Nagoya.
    • Year and Date
      2009-11-08
    • Related Report
      2010 Final Research Report
  • [Presentation] Development of a pH indicator immobilized gel-sheet for microenvironment analysis2009

    • Author(s)
      Taisuke MASUDA
    • Organizer
      20th IEEE International Symposium on Micro-Nano Mechatronics and Human Science
    • Place of Presentation
      Nagoya
    • Year and Date
      2009-11-08
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.biorobotics.mech.nagoya-u.ac.jp/index.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.biorobotics.mech.nagoya-u.ac.jp/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www-kiso.dent.tohoku.ac.jp/cfe/index.html

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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