| Project/Area Number |
21700476
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
HASHIMOTO Ken Kawasaki Medical School, 医学部, 助教 (80341080)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 動脈硬化 / 血管内皮細胞 / 単球 / 浸潤 / PECAM-1 / VE-cadherin / 内皮細胞 / マクロファージ / 分子イメージング |
|---|
| Research Abstract |
In atherogenesis, it is unclear whether and how one monocyte transmigration event affects endothelium to facilitate subsequent ones. When human monocytes were added twice to endothelial cells in vitro, significant augmentation of transmigration was observed at the second addition. Endothelial surface expressions of two major junctional molecules, PECAM-1 and VE-cadherin, increased and decreased respectively, in response to monocyte addition. These findings show that monocyte trans-endothelial migration alters endothelial junctional organization to more monocyte-permeable state (increased PECAM-1 and decreased VE-cadherin), resulting in the augmented transmigratory activity at a later stage.
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