Development of DNA chip by using nanogap

• Project/Area Number: 21700477
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Osaka Prefecture University
• Principal Investigator: TOKONAMI Shiho 大阪府立大学, 21 世紀科学研究機構, 講師 (60535491)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 金ナノ粒子 / DNA / センサ / ナノギャップ / ラベルフリー / 電気抵抗検出型 / 一塩基多型検出

Research Abstract

We have worked in research and development of biosensor using nanogap formed between adjacent gold nanoparticle (AuNP). A two-dimensional AuNP array was successfully fabricated by effectively utilizing chemical adsorption of thiol molecule and gold. Moreover, one-step preparation of nanogap was achieved by producing conductive polymer and AuNP at once. The obtained nanogap between AuNPs enabled us to electrochemically detect DNA and glucose.

Research Products

• [Journal Article] Organic-Inorganic Hybrid Nanoraspberry Consisted of Gold Nanoparticle and Aniline Oligomer (2011)
  • Author(s): R. Morita, R. Inoue, S. Tokonami, Y. Yamamoto, M. Nakayama, H. Nakao, H. Shiigi, T. Magaoka
  • Journal Title: J. Electrochem. Soc. Volume: 158
  • Peer Reviewed
• [Journal Article] Organic-Inorganic Hybrid Nanoraspberry Consisted of Gold Nanoparticle and Aniline Oligomer (2011)
  • Author(s): R.Morita, R.Inoue, S.Tokonami, Y.Yamamoto, M.Nakayama, H.Nakao, H.Shiigi, T.Nagaoka
  • Journal Title: J.Electrochem.Soc. Volume: 158
  • Peer Reviewed
• [Journal Article] Novel Synthesis, Structure, and Oxidation Catalysis of Ag/Au Bimetallic Nanoparticle (2010)
  • Author(s): S. Tokonami, N. Morita, K. Takasaki, N. Toshima
  • Journal Title: J. Phys. Chem. C Volume: 114 Pages: 10336-10341
  • Peer Reviewed
• [Journal Article] Novel Synthesis, Structure, and Oxidation Catalysis of Ag/Au Bimetallic Nanoparticle (2010)
  • Author(s): S.Tokonami, N.Morita, K.Takasaki, N.Toshima
  • Journal Title: J.Phys.Chem.C Volume: 114 Pages: 10336-10341
  • Peer Reviewed
• [Journal Article] Green Electroless Plating Method Using Gold Nanoparticles for Conducting Microbeads: Application to Anisotropic Conductive Films (2009)
  • Author(s): S. Tokonami, Y. Yamamoto, Y. Mizutani, I. Ota, H. Shiigi, T. Nagaoka
  • Journal Title: J. Electrochem. Soc. Volume: 156
  • Peer Reviewed
• [Journal Article] Fabrication of SmCo5 Alloy Magnetic Nanoparticles by Assistance of Copper and Their Magnetic Properties (2009)
  • Author(s): S. Tokonami, M. Kinjo, M. Inokuchi, N. Toshima
  • Journal Title: Chem. Lett. Volume: 38 Pages: 682-683
  • NAID: 130004425588
  • Peer Reviewed
• [Journal Article] Micro- and nanosized molecularly imprinted polymers for high-throughput analytical applications (2009)
  • Author(s): S. Tokonami, H. Shiigi, T. Nagaoka
  • Journal Title: Anal. Chim. Acta Volume: 641 Pages: 7-13
  • Peer Reviewed
• [Journal Article] Activity enhancement of a screen-printed carbon electrode by modification with agold nanoparticle for glucose determination (2009)
  • Author(s): M.Iwamoto, S.Tokonami, H.Shiigi, T.Nagaoka
  • Journal Title: Research on Chemical Intermediates 35 Pages: 919-930
  • Peer Reviewed
• [Journal Article] Green Electroless Plating Method Using Gold Nanoparticles for Conducting Microbeads : Application to Anisotropic Conductive Films (2009)
  • Author(s): S.Tokonami, Y.Yamamoto, Y.Mizutani, I.Ota, H.Shiigi, T.Nagaoka
  • Journal Title: J.Electrochem.Soc. 156
  • Peer Reviewed
• [Presentation] 長岡勉過酸化ポリピロール膜の作製と分子認識 (2011)
  • Author(s): 中土井祐, 床波志保, 細末健太, 椎木弘
  • Organizer: 分析展2011 ・ 科学機器展2011 Seperation Science 2011
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2011-09-06
• [Presentation] 過酸化ポリピロール膜の作製と分子認識 (2011)
  • Author(s): 中土井祐, 床波志保, 細末健太, 椎木弘, 長岡勉
  • Organizer: 分析展2011・科学機器展2011 Seperation Science 2011
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2011-09-06
• [Presentation] ナノギャップ電極を利用したバイオセンサの高感度化 (2011)
  • Author(s): 床波志保, 濱田大地, 中土井祐, 椎木弘, 長岡勉
  • Organizer: 東京コンファレンス2010
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2011-08-30
• [Presentation] 金属ナノ粒子間ギャップを利用したDNAセンサの開発 (2011)
  • Author(s): 床波志保, 椎木弘, 長岡勉
  • Organizer: 第58回応用物理学関係連合講演会
  • Place of Presentation: 神奈川工科大
  • Year and Date: 2011-03-24
• [Presentation] 分子鋳型過酸化ポリピロール膜を用いたウイルスセンサの開発 (2011)
  • Author(s): 中土井祐, 椎木弘, 長岡勉, 床波志保
  • Organizer: 第58回応用物理学関係連合講演会
  • Place of Presentation: 神奈川工科大
  • Year and Date: 2011-03-24
• [Presentation] Au/Ag二元系金属ナノ粒子の調製および構造解析・光応答解析 (2011)
  • Author(s): 濱田大地, 飯田琢也, 戸嶋直樹, 床波志保
  • Organizer: 第58回応用物理学関係連合講演会
  • Place of Presentation: 神奈川工科大
  • Year and Date: 2011-03-24
• [Presentation] 金ナノ粒子固定化電極を利用したDNAセンサの開発 (2010)
  • Author(s): 床波志保, 濱田大地, 中土井祐, 椎木弘, 長岡勉
  • Organizer: 東京コンファレンス2010
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2010-08-30
• [Presentation] 金ナノ粒子架橋構造を利用した電気抵抗型DNAセンサの開発 (2009)
  • Author(s): 床波志保, 椎木弘, 長岡勉
  • Organizer: 日本分析化学会第58年会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-09-24
• [Presentation] Development of Electrical DNA Detection System by Using Open Bridge-Structured Gold Nanoparticle Array (2009)
  • Author(s): S. Tokonami, I. Ota, S. Shirai, H. Shiigi, T. Nagaoka
  • Organizer: Flow Analysis XI
  • Place of Presentation: Pollensa, Mallorca, Spain
  • Year and Date: 2009-09-17
• [Presentation] Open Bridge-Structured Gold Nanoparticle Film for Electrical DNA Detection (2009)
  • Author(s): S.Tkonami, I. Ota, S. Shirai, H. Shiigi, T. Nagaoka
  • Organizer: Euro Analysis 2009
  • Place of Presentation: Innsbruck, Austria
  • Year and Date: 2009-09-08
• [Presentation] Open Bridge-Structured Gold Nanoparticle Film for Electrical DNA Detection (2009)
  • Author(s): S.Tokonami, I.Ota, S.Shirai, H.Shiigi, T.Nagaoka
  • Organizer: EURO ANALYSIS 2009
  • Place of Presentation: Innsbruck
• [Book] "Gold Nano Films: Synthesis, Characterization, and Potential Biomedical Applications" in "Nanostructured Thin Films and Sufaces" (2009)
  • Author(s): S.Tokonami, H. Shiigi, T. Nagaoka
  • Publisher: Wiley VCH
• [Book] Gold Nano Films : Synthesis, Characterization, and Potential Biomedical Applications (2009)
  • Author(s): S.Tokonami, H.Shiigi, T.Nagaoka
  • Total Pages: 8
  • Publisher: Wiley VCH
• [Patent(Industrial Property Rights)] 金属ナノ粒子集積構造体を利用した圧力検出装置, 温度検出装置, 圧力検出方法, および温度検出方法 (2010)
  • Inventor(s): 飯田琢也, 床波志保, 山本陽二郎, 椹木弘, 長岡勉
  • Industrial Property Rights Holder: 大阪府立大学
  • Industrial Property Number: 2010-273291
  • Filing Date: 2010-12-08
• [Patent(Industrial Property Rights)] 金属ナノ粒子集積構造体を利用した被検出物質の検出装置および方法 (2010)
  • Inventor(s): 床波志保, 飯田琢也, 山本陽二郎, 椎木弘, 長岡勉
  • Industrial Property Rights Holder: 大阪府立大学
  • Industrial Property Number: 2010-273284
  • Filing Date: 2010-12-08
• [Patent(Industrial Property Rights)] 金属ナノ粒子集積構造体を利用した圧力検出装置、温度検出装置、圧力検出方法、および温度検出方法 (2010)
  • Inventor(s): 飯田琢也, 床波志保, 山本陽二郎, 椎木弘, 長岡勉
  • Industrial Property Rights Holder: 大阪府立大学
  • Industrial Property Number: 2010-273291
  • Filing Date: 2010-12-08