Development of human pluripotent stem cells with high gene targeting efficiency
| Project/Area Number |
21700492
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Saitama Medical University |
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Principal Investigator |
SUZUKI Keiichiro Saitama Medical University, 医学部, 客員講師 (70433654)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 遺伝子ターゲッティング / ヒトiPS細胞 / 遺伝子ターゲッテング |
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| Research Abstract |
We used helper-dependent adenoviral vectors to knock-out KU80, LIG1 and LIG3 genes by homologous recombination in human induced pluripotent cells (hPSCs). Unlike heterozygous mutants of LIG1 and LIG3, which showed a tendency for sensitivities to a reagent causing DNA single-strand break, the KU80 heterozygous hiPSCs did not show any obvious phenotypes. Furthermore, increase in gene targeting efficiency was not observed in these heterozygous mutants.
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Report
(3 results)
Research Products
(6 results)
