| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Muscular necrosis was produced in the soleus muscle of adult rats by intramuscular injection of cardiotoxin and subsequent regeneration was induced. The sampling of soleus muscle was performed on 14th day after the injection. Immunohistochemical staining of phosphorylated HSP27 at serine 15 (p-Ser15) and some myogenic marker proteins was performed in the cross section of the soleus muscle. As the results, strong expression of p-Ser15 was noted in 20% of all fibers observed in the regenerated muscle. Further, most of these p-Ser15-positive fibers were myotubes or small immature muscle fibers with the cross-sectional area of less than 400μm^2. On the other hands, the fibers without expression of p-Ser15 were also seen in the regenerated muscle. These results suggested that p-Ser15-positive cells which was present during muscular regeneration were susceptive to the cellular stress due to the muscle activity.
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