Assessment method of food that control postprandial hyperglycemia using a DNA microarray

• Project/Area Number: 21700693
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied health science
• Research Institution: The University of Tokushima
• Principal Investigator: OKUMURA Hisami The University of Tokushima, 大学院・ヘルスバイオサイエンス研究部, 助教 (30322259)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 生活習慣表 / 食後高血糖 / DNAチップ / トランスクリプトミクス / ヒト試験 / 機能性食品

Research Abstract

The objective of this study was to elucidate whether intake of foods differed from glycemic and insulinemic responses affect gene expression profiling in peripheral white blood cells (WBC). Futhermore, we observed the time course of gene expression changes in WBC to elucidate the metabolic changes in the postprandial state. In randomized crossover study, seven healthy subjects consumed glucose (GL), white rice (WR) and rolled barley (BAR) containing 75 g of available carbohydrate and water (WAT). Blood glucose, insulin and free fatty acid concentrations, subjective levels of fullness and hunger, and Gene expression profiling in WBC were measured. The number of genes changed more than 1.5-fold and the expression patterns in the time-course were different among the GL, WR and BAR. Several genes involved in glycolysis and fatty acid ss-oxidation were markedly changed after intake of the GL, WR and BAR. These genes were not changed at all time points in the WAT. It is concluded from this study that gene expression profiling in WBC can reflect food-related metabolic changes even in the postprandial state.

Research Products

• [Journal Article] Does-dependent effects of barley cooked with white rice on post prandial glucose and desacyl ghrelin levels (2009)
  • Author(s): M Sakuma, et al.
  • Journal Title: J Clin Biochem Nutr 44 Pages: 151-159
  • Peer Reviewed
• [Presentation] DNAチップを用いた食品負荷による食後高血糖の網羅的遺伝子発現評価 (2011)
  • Author(s): 川上由香、奥村仙示、森由佳、足立知咲、佐久間理英、武田英二
  • Organizer: 第14回日本病態栄養学会
  • Place of Presentation: 横浜
• [Presentation] 食事組成の違いが食後高血糖上昇および代謝に及ぼす影響 (2011)
  • Author(s): 奥村仙示
  • Organizer: 第14回日本病態栄養学会
  • Place of Presentation: 横浜
• [Presentation] DNAチップを用いた食品負荷による食後高血糖の網羅的遺伝子発現評価 (2011)
  • Author(s): 川上由香
  • Organizer: 病態栄養学会
  • Place of Presentation: 横浜市
• [Presentation] 食事組成の違いが食後高血糖上昇および代謝に及ぼす影響 (2011)
  • Author(s): 奥村仙示
  • Organizer: 病態栄養学会
  • Place of Presentation: 横浜市
• [Presentation] Gene expression profiling of human peripheral blood is affected by the differences in glycemic and insulinemic responses to food intake. (2010)
  • Author(s): Y Kawakami, H Yamanaka-Okumura, M Sakuma, Y Matsumoto, T Sato, E Takeda
  • Organizer: 32th ESPEN congress
  • Place of Presentation: Nice, France
• [Presentation] Gene expression profiling of human peripheral blood is affected by the differences in glycemic and insulinemic responses to food intake (2010)
  • Author(s): 川上由香
  • Organizer: ESPEN
  • Place of Presentation: フランス・ニース