| Project/Area Number |
21700701
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied health science
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| Research Institution | Juntendo University |
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Principal Investigator |
HAGIWARA Shinji Juntendo University, 医学部, 助教 (70445568)
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| Co-Investigator(Kenkyū-buntansha) |
TANIMOTO Mituo , 医学部, 助教 (00420836)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | メタボリックシンドローム / 慢性腎臓病 / Azgp-1 / Zinc-alpha2-glycoprotein (Azgp-1) / PPARγ / Zinc-alpha2-glycoprotein(Azgp-1) |
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| Research Abstract |
In this study, we identified the localization of Azgp-1 expression in the kidney of spontaneous type 2 diabetic obesity KK/Ta mouse, analyzed the function of Azgp-1 in the kidney and examined the role of Azgp-1 on CKD with metabolic syndrome.
The expressions of Azgp-1 protein were increased at tubular epithelial cells of KK/Ta mice compared to BALBc mice. There are no expressions of Azgp-1 protein in the kidney of STZ induced diabetic BALBc mice. The Azgp-1 mRNA expressions were decreased in the kidney of high fat diet C57BL/6 mice. The Azgp-1 mRNA expressions were increased in the kidney of KK/Ta mice treated by Pioglitazone. The concentrations of urinary Azgp-1 protein were significantly increased in patients with type 2 diabetes and positively correlated with glomerular filtration rate (GFR), urinary albumin creatinine ratio and urinary NAG creatinine ratio.
Azgp-1 protein expressions were increased in proximal tubular epithelial cells of diabetic nephropathy, probably related to lipid metabolism and transcript factor PPAR-γ is a modulator of Azgp-1 in the kidney. Moreover, it is suggested that the concentrations of urinary Azgp-1 is useful as a new urinary biomarker in type 2 diabetic nephropathy patients.
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