Involvement of microRNA in osteoblast differentiation, and its application

• Project/Area Number: 21700715
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied health science
• Research Institution: Kinki University (2011); Gifu International Institute of Biotechnology (2009-2010)
• Principal Investigator: ITOH Tomohiro 近畿大学, 農学部, 講師 (30435854)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 加齢・老化 / microRNA / 骨芽細胞 / 分化

Research Abstract

In this study, we found that the expression levels of 9 miRNAs(log ratio, 1.5<) were distinct between BMP-2-treated and nontreated cells. Among these, we showed that Dlx5, Ets1 BMP-2 and Ets1 genes were regulated by miR-141/-200a, miR-208, and miR-370, respectively. The above-mentioned results strongly suggests that miRNAs has been closely involved in BMP-2-induced osteoblast differentiation.

Research Products

• [Journal Article] MicroRNA-208 Modulates BMP-2-stimulated Mouse Pre-osteoblast Differentiation by Direct Targeting V-ets Erythroblastosis Virus E26 Oncogene Homolog 1 (2010)
  • Author(s): Tomohiro Itoh, Takeda Shu, Yukihiro Akao
  • Journal Title: J. Biol. Chem. Volume: 285(36) Pages: 27745-27752
  • Peer Reviewed
• [Journal Article] MicroRNA-208 modulates BMP-2-stimulated mouse pre-osteoblast differentiation by direct targeting V-ets erythroblastosis virus E26 oncogene homolog 1. (2010)
  • Author(s): Tomohiro Itoh, Takeda Shu, Yukihiro Akao.
  • Journal Title: The Journal of Biological Chemistry Volume: 285(36) Pages: 27745-27752
  • Peer Reviewed
• [Journal Article] MicroRNA-141 and-200a are involved in bonemorphogenetic protein-2-induced Mouse Pre-osteoblast Differentiation by Targeting distal-less homeobox 5 (2009)
  • Author(s): Tomohiro Itoh, Yoshinori Nozawa, Yukihiro Akao
  • Journal Title: J. Biol. Chem. Volume: 284(29) Pages: 19272-19279
  • Peer Reviewed
• [Journal Article] MicroRNA-141 and -200a are involved in bone morphogenetic protein-2-induced mouse pre-osteoblast differentiation by targeting distal-less homeobox 5. (2009)
  • Author(s): Itoh T., Nozawa Y., Akao Y.
  • Journal Title: The Journal of Biological Chemistry 284 Pages: 19272-19279
  • Peer Reviewed
• [Journal Article] Expression of BMP-2 and Ets1 in BMP-2-stimulated mouse pre-osteoblast differentiation is regulated by microRNA-370
  • Author(s): Tomohiro Itoh, Masashi Ando, Yasuyuki Tsukamasa, Yukihiro Akao
  • Journal Title: FEBS Lett Volume: (in press)
  • Peer Reviewed
• [Presentation] microRNA-370によるBMP-2誘導マウス前駆骨芽細胞分化調節 (2012)
  • Author(s): 伊藤智広、安藤正史、塚正泰之、赤尾幸博
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2012-03-29
• [Presentation] microRNA-370によるBMP-2誘導マウス前駆骨芽細胞分化調節 (2012)
  • Author(s): 伊藤智広、安藤正史、塚正泰之、赤尾幸博
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
• [Presentation] microRNA-208によるBMP-2誘導マウス前駆骨芽細胞分化調節 (2010)
  • Author(s): 伊藤智広、竹田秀、赤尾幸博
  • Organizer: 2010年度日本分子生物・日本生化学会合同大会
  • Place of Presentation: 神戸
• [Presentation] 骨芽細胞分化におけるmicroRNAの役割 (2010)
  • Author(s): 伊藤智広
  • Organizer: 岐阜大学大学院先端創薬シンポジウム
  • Place of Presentation: 岐阜
• [Presentation] microRNA-208によるBMP-2誘導マウス前駆骨芽細胞分化調節 (2010)
  • Author(s): 伊藤智広、竹田秀、赤尾幸博
  • Organizer: 2010年度日本分子生物・日本生化学会合同大会
  • Place of Presentation: 神戸国際会議場
• [Presentation] BMP-2誘導マウス前駆骨芽細胞の分化調節に関わるmicroRNAの同定 (2009)
  • Author(s): 伊藤智広、大口健司、野澤義則、赤尾幸博
  • Organizer: 2009年度日本農芸化学会
  • Place of Presentation: 福岡
• [Remarks]
  • URL: http://www.giib.or.jp
• [Patent(Industrial Property Rights)] 間葉系細胞の分化調節剤およびこれを用いた医薬、並びに間葉系細胞への分化調節作用を有する物質のスクリーニング方法 (2011)
  • Inventor(s): 伊藤智広、赤尾幸博、村瀬博宣
  • Industrial Property Rights Holder: 岐阜大学、シーシーアイ株式会社
  • Industrial Property Number: 2011-234162
  • Filing Date: 2011-10-25
• [Patent(Industrial Property Rights)] 間葉系細胞分化を調節するための薬剤及びその利用 (2011)
  • Inventor(s): 伊藤智広, 赤尾幸博, 村瀬博宜
  • Industrial Property Rights Holder: 岐阜大学シーシーアイ株式会社
  • Industrial Property Number: 2011-234162
  • Filing Date: 2011-10-25
• [Patent(Industrial Property Rights)] 間葉系細胞分化を調節するための薬剤及びその利用 (2009)
  • Inventor(s): 伊藤智広, 赤尾幸博, 三島敏
  • Industrial Property Rights Holder: 岐阜大学
  • Filing Date: 2009-11-19