| Project/Area Number |
21700751
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Eating habits, studies on eating habits
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| Research Institution | Ochanomizu University |
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Principal Investigator |
SONE Yasuko お茶の水女子大学, 生活環境教育研究センター, 助教 (80452027)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | L-アスコルビン酸 / ナトリウム依存性ビタミンCタランスポーター / デヒドロア / ChIP / ODSラット / デヒドロアスコルビン酸レダクターゼ / ビタミンCトランスポーター / DNAマイクロアレイ / 遺伝子発現 |
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| Research Abstract |
We investigated the effects of L-ascorbic acid(AsA) administration(0, 5 and 100 mg/day) on the expression levels of sodium-dependent vitamin C transporters(SVCTs) and that of other genes in ODS rat livers. Similarly, the effect of AsA addition into the medium on the transporters expressions in rat primary hepatocyte and HepG2 was examined. Moreover, to examine the interaction between the promoter regions of SVCTs and the transcriptional factors, ChiP IP & qPCR assay was conducted.
As a result, the SVCT1 levels increased in the livers of rats not administered AsA and decreased in the livers of rats administered 100 mg/day AsA, indicating that these transporter levels can be influenced by the amount of AsA administered. Also, these transporter levels can be associated with redox in vivo. The ChiP IP & qPCR assay exhibited the binding between of C/EBP and the promoter region of SVCT2.
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