The effect of L-ascorbic acid on transcriptional regulation

• Project/Area Number: 21700751
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Eating habits, studies on eating habits
• Research Institution: Ochanomizu University
• Principal Investigator: SONE Yasuko お茶の水女子大学, 生活環境教育研究センター, 助教 (80452027)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: L-アスコルビン酸 / ナトリウム依存性ビタミンCタランスポーター / デヒドロア / ChIP / ODSラット / デヒドロアスコルビン酸レダクターゼ / ビタミンCトランスポーター / DNAマイクロアレイ / 遺伝子発現

Research Abstract

We investigated the effects of L-ascorbic acid(AsA) administration(0, 5 and 100 mg/day) on the expression levels of sodium-dependent vitamin C transporters(SVCTs) and that of other genes in ODS rat livers. Similarly, the effect of AsA addition into the medium on the transporters expressions in rat primary hepatocyte and HepG2 was examined. Moreover, to examine the interaction between the promoter regions of SVCTs and the transcriptional factors, ChiP IP & qPCR assay was conducted.
As a result, the SVCT1 levels increased in the livers of rats not administered AsA and decreased in the livers of rats administered 100 mg/day AsA, indicating that these transporter levels can be influenced by the amount of AsA administered. Also, these transporter levels can be associated with redox in vivo. The ChiP IP & qPCR assay exhibited the binding between of C/EBP and the promoter region of SVCT2.

Research Products

• [Journal Article] The level of orally ingested vitamin Caffected the expression of vitamin Ctransporters and vitamin Caccumulation in the livers of ODS rats (2011)
  • Author(s): Sone Y, Ueta E, Kodama S, Sannoumaru Y, Miyake N, Sone H, Fujiwara Y, Otsuka Y, Kondo K, Inagaki M, Namba E, Kurata T, Suzuki E
  • Journal Title: Biosci Biotechnol Biochem Volume: 75 Pages: 2394-7
  • NAID: 120005520667
  • Peer Reviewed
• [Journal Article] Dose of 3-methylcholanthrene enhancesvitamin C accumulation and mRNA expression of its transporter in theliver of ODS rats and in HepG2 cells (2011)
  • Author(s): Sone Y, Ueta E, Sannomaru Y, Miyake N, Sone H, Otsuka Y, Kondo K, Kurata T, Suzuki E
  • Journal Title: J Biochem Mol Toxicol Volume: 25 Pages: 369-76
  • Peer Reviewed
• [Journal Article] The Level of Orally Ingested Vitamin C Affected the Expression of Vitamin C Transporters and Vitamin C Accumulation in the Livers of ODS Rats (2011)
  • Author(s): Sone Y, Ueta E, Kodama s, Sannoumaru Y, Miyake N, Sone H, Fujiwara Y, Otsuka Y, Kondo K, Inagaki M, Nanba E, Kurata T, Suzuki E
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 75 Issue: 12 Pages: 2394-2397
  • DOI: 10.1271/bbb.110312
  • NAID: 10030397928
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Dose of 3-methylcholanthrene enhances vitamin C accumulation and mRNA expression of its transporter in the liver of ODS rats and in HepG2 cells (2011)
  • Author(s): Sone Y, Ueta E, Sannomaru Y, Miyake N, Sone H, Otsuka Y, Kondo K, Kurata T, Suzuki E
  • Journal Title: J Biochem Mol Toxicol Volume: 25 Issue: 6 Pages: 369-76
  • DOI: 10.1002/jbt.20403
  • Peer Reviewed
• [Journal Article] Antioxidant/anti-inflammatory activities and total phenolic content of extracts obtained from plants grown in Vietnam. (2011)
  • Author(s): Yasuko Sone, Joon-Kwan Moon, Truong Tuyet Mai, Nghiem Nguyet Thu, Eri Asano, Keiko Yamaguchi, Yuzuru Otsuka, Takayuki Shibamoto
  • Journal Title: Journal of the Science of Food and Agriculture. Volume: (未定 印刷中(掲載確定))
  • Peer Reviewed
• [Journal Article] Effect of oxygen concentration on the expression of antioxidant gene in HepG2. (2010)
  • Author(s): Yasuko SONE, Yasuko KUDO, Rieko OBA, Yuzuru OTSUKA
  • Journal Title: Biosci.Biotechnol.Biochem. Volume: 74 Pages: 1267-70
  • NAID: 10027556534
  • Peer Reviewed
• [Journal Article] Association with Lifestyle Factor, Polymorphisms in Adiponectin, Perilipin and Hormone Sensitive Lipase, and Clinical Markers in Japanese Male. (2010)
  • Author(s): Yasuko SONE, Keiko YAMAGUCHI, Aya FUJIWARA, Toshimi KIDO, Kazuo KAWAHARA, Asako, ISHIWAKI, Kazuo KONDO, yutaka MORITA, Noriko TOMINAGA, Yuzuru OTSUKA
  • Journal Title: Journal of Nutritional Science and Vitaminology Volume: 56 Pages: 123-31
  • Peer Reviewed
• [Journal Article] Effect of oxygen concentration on the expression of antioxidant gene in HepG2 (2010)
  • Author(s): 曽根保子、工藤靖子、大場理恵子、大塚譲
  • Journal Title: Bioscience, Biotechnology, and Biochemistry (in press, 未定)
  • NAID: 10027556534
  • Peer Reviewed
• [Journal Article] Association with Lifestyle Factor, Polymorphisms in Adiponectin, Perilip in and Hormone Sensitive Lipase, and Clinical Markers in Japanese Male (2010)
  • Author(s): 曽根保子、山口敬子、藤原綾、貴堂としみ、河原和夫、石脇亜沙子、近藤和雄、森田寛、冨永典子、大塚譲
  • Journal Title: Journal of Nutritional Science and Vitaminology 56(未定)
  • Peer Reviewed
• [Presentation] Anti-oxidant/anti-inflammatory activities and total phenolic content of extracts obtained from plants grown in Vietnam (2012)
  • Author(s): Yasuko Sone, Joon-Kwan Moon, Truong Tuyet Mai, Nghiem Nguyet Thu, Eri Asano, Keiko Yamaguchi, Yoko Fujiwara, Yuzuru Otsuka, Takayuki Shibamoto
  • Organizer: International Conference on Food Factors
  • Place of Presentation: 台湾・Taipei International Convention Center
  • Year and Date: 2012-11-22
• [Presentation] 脂質代謝関連遺伝子FADS、PPARγの遺伝子多型が臨床指標に及ぼす影響 (2011)
  • Author(s): 曽根保子、園田麻里子、貴堂としみ、市育代、河原和夫、森田寛、近藤和雄、大塚譲、藤原葉子
  • Organizer: 日本臨床栄養学会
  • Place of Presentation: 東京・都市センターホテル
  • Year and Date: 2011-10-28
• [Presentation] 日本人男性における脂質代謝関連遺伝子多型と臨床指標との関連性 (2011)
  • Author(s): 曽根保子、貴堂としみ、河原和夫、大塚譲、藤原葉子
  • Organizer: 日本栄養・食糧学会
  • Place of Presentation: 東京・お茶の水女子大学
  • Year and Date: 2011-05-15
• [Presentation] RNAi法による酸化ストレス応答遺伝子ノックダウンによる細胞増殖の影響 (2010)
  • Author(s): 曽根保子、宮倉玲子、大塚譲
  • Organizer: 第62回 日本家政学会大会
  • Place of Presentation: 広島大学
  • Year and Date: 2010-05-30
• [Presentation] Influence of Vitamin C Level on the Gene Expression in Primary Hepat ocyte of ODS Rat. (2009)
  • Author(s): 曽根保子、渡邉雅信、鬼武ふみ子、叔〓、児玉暁、齋藤和美、山王丸靖子、上田悦子, 他
  • Organizer: 19th International Congress of Nutrition
  • Place of Presentation: BITEC, Bangkok, Thailand
  • Year and Date: 2009-10-08
• [Presentation] Influence of Dose Level of Vitamin C onthe Expression of Vitamin C Transporters in ODS Rats (2009)
  • Author(s): Sone Y, Midorikawa A, Taniguchi S, Watanabe M, Shu M, Onitake F, Kodama S, Saito K, Sannoumaru Y, Ueta E, Miyake N, Sone H, Namba E, Otsuka Y, Kondo K, Inagaki M, Kurata T, Suzuki E
  • Organizer: 19^<th> InternationalCongress of Nutrition
  • Place of Presentation: Bangkok, Thailand
• [Presentation] Effect of 3-MC and L-Ascorbic Acid onGene Expression of Primary Human Hepatocyte (2009)
  • Author(s): Sone Y, Iwaki E, Otsuka Y
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] アスコルビン酸濃度の違いによる遺伝子発現変化の検討 (2009)
  • Author(s): 曽根保子、丑田晴菜、児玉暁、齋藤和美、山王丸靖子、三宅紀子、曽根博仁、倉田忠男、鈴木恵美子
  • Organizer: 日本ビタミン学会第61回大会
  • Place of Presentation: 京都
• [Remarks]
  • URL: http://www.hles.ocha.ac.jp/food/eiyoken/index.html
• [Remarks]
  • URL: http://www.cf.ocha.ac.jp/ieshl/otsuka/pub/index.htm
• [Remarks]
  • URL: http://pea.ieshl.ocha.ac.jp/