Developing a novel bioassay method using surface-enhanced laser desorption/ionization mass spectrometry for detecting toxic materials.

• Project/Area Number: 21710070
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: AOKI Motohide 東京薬科大学, 生命科学部, 助教 (30418917)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 環境分析 / 質量分析法 / 環境評価 / バイオマーカー

Research Abstract

For more effective and efficient chemical risk management, a novel bioassay method for detecting toxic materials in the environment using surface-enhanced laser desorption/ionization mass spectrometry(SELDI-MS) was developed. The established SELDI-MS protocol for collecting the response patterns to toxic materials in the protein profile of microalgae as a biological indicator of environment allows to efficiently searching for biomarkers responding to toxic materials and to detecting the hazardous substances.

Research Products

• [Journal Article] Involvement of sulfoquinovosyl diacylglycerol in DNA synthesis in Synechocystis sp.PCC 6803 (2012)
  • Author(s): M.Aoki, M.Tsuzuki, N.Sato
  • Journal Title: BMC Research Notes Volume: 0 Issue: 1 Pages: 98-98
  • DOI: 10.1186/1756-0500-5-98
  • Peer Reviewed
• [Journal Article] Observation of Cell-Size Variation under Environmental Stress by Fluorescence Correlation Spectroscopy without Objective Image Magnification (2012)
  • Author(s): K.Fujiwara, K.Horiuchi, S.Goryoda, Y.Hashidume, N.Horiguchi, T.Miyakawa, M.Takasu、 M. Aoki
  • Journal Title: Journal of Environmental Science and Engineering (A & B) Volume: 1 Pages: 364-370
  • Peer Reviewed
• [Journal Article] Thallium induces morphological changes in the photosynthetic apparatus of Synehocystis sp. PCC6803 (2010)
  • Author(s): M. Aoki, H. Matsumoto, T. Takahashi, K. Sato, H. Kumata, K. Fujiwara
  • Journal Title: Proceedings of 15th International Congress on Photosynthesis Volume: (in press)
• [Journal Article] 天然水中有機物の光分解装置の政策と化学発光法を用いたその性能評価 (2010)
  • Author(s): 藤原祺多夫、大迫祐太、佐々木孝菜、熊田英峰、青木元秀、川島範男
  • Journal Title: 分析化学 Volume: 59(11) Pages: 1021-1028
  • Peer Reviewed
• [Journal Article] Thallium Induces Morphological Changes in the Photosynthetic Apparatus of Synehocystis sp.PCC6803
  • Author(s): M.Aoki, H.Matsumoto, T.Takahashi, K.Sato, H.Kumata, K.Fujiwara
  • Journal Title: In the PS2010 Proceedings, 15th International Congress on Photosynthesis Held at Beijing, China Volume: (印刷中)
• [Presentation] SELDI-MSを用いたシアノバクテリアの環境ストレスバイオマーカーの探索 (2012)
  • Author(s): 青木元秀、藤原祺多夫
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
• [Presentation] 低pH条件における三価クロム曝露はクロレラの葉緑体膜の異常を引き起こす (2012)
  • Author(s): 高尾有希乃、青木元秀、熊田英峰、藤原祺多夫
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
• [Presentation] 低pH条件における三価クロム曝露がクロレラの形態に与える影響 (2011)
  • Author(s): 高尾有希乃, 青木元秀, 熊田英峰, 藤原祺多夫
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京大学(駒場)
• [Presentation] Windowウォッチによる大気中PAHsの分布調査:東京都多摩地区の小学校でのケーススタディ (2011)
  • Author(s): 浜出早紀, 伊藤麻南実, 熊田英峰, 青木元秀, 藤原祺多夫
  • Organizer: 第20回日本環境化学討論会
  • Place of Presentation: 熊本県立大学
• [Presentation] パッシブエアサンプラー(PAS)を用いた東京都心・郊外における大気中PAHの季節変動評価 (2011)
  • Author(s): 伊藤麻南実, 熊田英峰, 青木元秀, 藤原祺多夫
  • Organizer: 第20回日本環境化学討論会
  • Place of Presentation: 熊本県立大学
• [Presentation] 堆積物中PAHsのPCGC単離のための前処理法:UCM除去 (2011)
  • Author(s): 斎藤祥一, 熊田英峰, 青木元秀, 藤原祺多夫
  • Organizer: 第20回日本環境化学討論会
  • Place of Presentation: 熊本県立大学
• [Presentation] Gene expression analyses of putative chromate transporters and stress response factors under chromate exposure condition in a cyanobacteria (2010)
  • Author(s): K. Takenaka, A. Kikuchi, M. Aoki, H. Kumata, K. Fujiwara
  • Organizer: 11th European Meeting on Environmental Chemistry
  • Place of Presentation: Portoroz, Slovenia
• [Presentation] Thallium induces morphological changes in the photosynthetic membrane of Synechocystis sp. PCC6803 (2010)
  • Author(s): H. Matsumoto, M. Aoki, T. Takahashi, K. Sato, H. Kumata, K. Fujjiwara
  • Organizer: 15th International Congress of Photosynthesis
  • Place of Presentation: Beijing, China
• [Presentation] Thallium induces morphological changes in the photosynthetic membrane of Synechocystis sp.PCC6803 (2010)
  • Author(s): H.Matsumoto, M.Aoki, T.Takahashi, K.Sato, H.Kumata, K.Fujjiwara
  • Organizer: 15th International Congress of Photosynthesis
  • Place of Presentation: Beijing, China
• [Presentation] Gene Expression Analyses of Putative Chromate Transporters and Stress Response Factors under Chromate Exposure Condition in a Cyanobacteria (2010)
  • Author(s): K.Takenaka, A.Kikuchi, M.Aoki, H.Kumata, K.Fujiwara
  • Organizer: 11th European Meeting on Environmental Chemistry
  • Place of Presentation: Portoroz, Slovenia
• [Presentation] Determination of Cell-size variation under the Environmental Stress by Fluorescence Correlation Spectroscopy without Image Magnification (2010)
  • Author(s): K.Horiuchi, S.Goryoda, N.Horiguchi, M.Aoki, K.Fujiwara
  • Organizer: 11th European Meeting on Environmental Chemistry
  • Place of Presentation: Portoroz, Slovenia
• [Presentation] パッシブエアサンプラー(PAS)を用いた大気中PAH汚染起源解明のための分析方法の最適化 (2010)
  • Author(s): 伊藤麻南実, 熊田英峰, 青木元秀, 藤原祺多夫
  • Organizer: 第19回環境化学討論会
  • Place of Presentation: 中部大学
• [Presentation] 多摩丘陵森林土壌中のリン脂質脂肪酸の定量的分析方法の開発 (2010)
  • Author(s): 吉田伸一郎, 熊田英峰, 青木元秀, 藤原祺多夫
  • Organizer: 第19回環境化学討論会
  • Place of Presentation: 中部大学
• [Presentation] Analysis of proteins responded to thallium exposure in Synechocystis sp. PCC6803 (2009)
  • Author(s): T. Takahashi, M. Aoki, K. Fujiwara
  • Organizer: 10^<th> European Meeting on Environmental Chemistry
  • Place of Presentation: Limoges, France
• [Presentation] Toxicity of cobalt complex isomers to micro-algae (2009)
  • Author(s): N. Sa-ai, M. Midorikawa, M. Aoki, K. Fujiwara
  • Organizer: 10^<th> European Meeting on Environmental Chemistry
  • Place of Presentation: Limoges, France
• [Presentation] Synechocystis sp. PCC6803におけるタリウムストレス応答タンパク質の解析 (2009)
  • Author(s): 高橋達矢、末松仁、青木元秀、熊田英峰、藤原祺多夫
  • Organizer: 日本植物学会第73回大会
  • Place of Presentation: 山形大学
• [Presentation] クロムストレスがChlorella kessreriの生体膜脂質におよぼす影響とpH条件 (2009)
  • Author(s): 青木元秀、笹本勝紀、江田歩美、熊田英峰、藤原祺多夫
  • Organizer: 日本植物学会第73回大会
  • Place of Presentation: 山形大学
• [Presentation] Synechocystis sp. PCC6803におけるクロムストレス応答遺伝子の探索 (2009)
  • Author(s): 竹中一貴、菊池明人、青木元秀、熊田英峰、藤原祺多夫
  • Organizer: 日本植物学会第73回大会
  • Place of Presentation: 山形大学
• [Presentation] シアノバクテリアchrA相同遺伝子の六価クロム輸送能と発現量解析 (2009)
  • Author(s): 菊池明人、竹中一貴、青木元秀、熊田英峰、藤原祺多夫
  • Organizer: 日本植物学会第73回大会
  • Place of Presentation: 山形大学
• [Presentation] コバルト錯体のクロレラに対する毒性研究 (2009)
  • Author(s): 佐合奈菜江、緑川真以子、青木元秀、藤原祺多夫
  • Organizer: 日本分析化学会第58年会
  • Place of Presentation: 北海道大学
• [Presentation] Identification of stress response genes to hexavalent chromium in cyanobacterium Synechocystis sp. PCC6803 (2009)
  • Author(s): Takenaka, K., Aoki, M., Fujiwara, K.
  • Organizer: 10^<th> European Meeting on Environmental Chemistry
  • Place of Presentation: Limoges, France
• [Presentation] Analysis of the transporters of hexavalent chromium in cyanobacterium Synechocystis sp. PCC6803 (2009)
  • Author(s): Kikuchi, A., Aoki, M., Fujiwara, K.
  • Organizer: 10^<th> European Meeting on Environmental Chemistry
  • Place of Presentation: Limoges, France
• [Remarks]
  • URL: http://logos.ls.toyaku.ac.jp/.aoki/seldi/