| Project/Area Number |
21710075
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Nagaoka University of Technology |
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Principal Investigator |
ABE Katsumasa 長岡技術科学大学, 工学部, 助教 (40509551)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 環境修復技術 / 環境汚染物質 / 有機リン化合物 / 加水分解酵素 / 発現機構 / 遺伝子クローニング / 環境浄化 / 難分解性環境汚染物 / 有機リン加水分解酵素 / 遺伝子破壊株 / 発現機構解析 / 難分解性環境汚染物質 |
|---|
| Research Abstract |
Tris(1, 3-dichloro-2-propyl) phosphate(TDCPP), a typical organophosphate triesters, has been used worldwide as flame-retardants, and thus resulting in its dispersion into natural environment. Since this compound is known to possess carcinogenicity, mutagenicity and neurotoxicity, persistence of TDCPP in natural environment has been concerned. In this study, I report the purification and characterization of TDCPP-degrading enzyme from Sphingomonas sp. TDK1 and its gene cloning and sequencing. The enzyme can degrade not only the trihaloalkyl phosphates but also aryl organophosphates. The enzyme was induced when environmental orthophosphate becomes limiting.
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