Production of lactate polymer in the transgenic plants

• Project/Area Number: 21710087
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Environmental technology/Environmental materials
• Research Institution: Hokkaido University
• Principal Investigator: MATSUMOTO Kenichiro 北海道大学, 大学院・工学研究院, 准教授 (80360642)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: バイオポリマー / 組換え植物 / 乳酸 / 組み換え植物

Research Abstract

Polylactic acid is a biobased polyester, which is synthesized from fermentative lactate. We attempted to synthesize lactate-incorporated polymer using the transgenic plants. To meet this goal, the gene encoding the monomer supplying enzymes and polymerase were introduced into plants. The expression of these genes and polyester synthesis were confirmed.

Research Products

• [Journal Article] Improved polyhydroxybutyrate (PHB) production in transgenic tobacco by enhancing translation efficiency of bacterial PHB biosynthetic genes (2011)
  • Author(s): Ken'ichiro MATSUMOTO, Kenjiro MORIMOTO, Aoi GOHDA, Hiroaki SHIMADA, Seiichi TAGUCHI
  • Journal Title: J. Biosci. Bioeng. Volume: Vol. 111(4) Pages: 485-488
  • Peer Reviewed
• [Journal Article] Biosynthesis of glycolate-based polyesters containing medium-chain-length 3-hydroxyalkanoates in recombinant Escherichia coli expressing engineered polyhydroxyalkanoate synthase (2011)
  • Author(s): Ken'ichiro MATSUMOTO, Ayako ISHIYAMA, Kohei SAKAI, Tetsufumi SHIBA, Seiichi TAGUCHI
  • Journal Title: J. Biotechnol. Volume: Vol.156(3) Pages: 214-217
  • Peer Reviewed
• [Journal Article] Improved polyhydroxybutyrate (PHB) production in transgenic tobacco by enhancing translation efficiency of bacterial PHB biosynthetic genes (2011)
  • Author(s): K.Matsumoto, K.Morimoto, A.Gohda, H.Shimada, S.Taguchi
  • Journal Title: Journal of Bioscience and Bioengineering Volume: (Web release)
  • Peer Reviewed
• [Presentation] 高活性化PhaBの導入による組換えタバコでのPHB生産性の向上 (2011)
  • Author(s): 大場貴史、江口綾、森本健二郎、本橋廉、松本謙一郎、田口精一
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都
• [Presentation] 進化型PhaBによる組換えタバコにおけるPHB生産性増強 (2011)
  • Author(s): 大場貴史、江口綾、森本健二郎、本橋廉、松本謙一郎、田口精一
  • Organizer: 日本生物工学会大会
  • Place of Presentation: 東京
• [Presentation] 組換え大腸菌による乳酸およびグリコール酸ベース新奇バイオプラスチックの生合成 (2011)
  • Author(s): 石山絢子, 松本謙一郎, 田口精一
  • Organizer: 日本生物工学会大会
  • Place of Presentation: 東京
• [Presentation] 乳酸およびグリコール酸ベース新奇バイオプラスチックの微生物生産 (2011)
  • Author(s): 斯波哲史、石山絢子、松本謙一郎、田口精一
  • Organizer: 農芸化学会北海道支部 学術講演会
  • Place of Presentation: 札幌
• [Presentation] Engineered enzymes and pathways for production of lactate-based polyesters (2010)
  • Author(s): Ken'ichiro MATSUMOTO, Miwa YAMADA, Fumi SHOZUI, Shu URAMOTO, Ren MOTOHASHI, Takanori NAKAI, Kotaro SHIMIZU, Seiichi TAGUCHI
  • Organizer: International Conference on Cellular & Molecular Bioengineering 2
  • Place of Presentation: Nanyang Technological University, Singapore
• [Presentation] PHB生合成系酵素群の発現効率化による組換え体タバコでのPHB生産性の向上 (2009)
  • Author(s): 森本健二郎、合田葵、松本謙一郎、島田浩章、田口精一
  • Organizer: 農芸化学会北海道支部学術講演会
  • Place of Presentation: 札幌
• [Presentation] Expansion of Bological Polyesters by Engineering of Microbial Polyester Synthase (2009)
  • Author(s): Ken'ichiro Matsumoto
  • Organizer: 2009 Taiwan-Japan Biateral Polymer Symposium
  • Place of Presentation: Tiwan
• [Remarks]
  • URL: http://www.eng.hokudai.ac.jp/labo/seika/TOP.html