Development of peptide-immobilized TiO2 chip for monitoring of intracellular protein phosphorylation signals.
| Project/Area Number |
21710121
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Kitakyushu National College of Technology |
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Principal Investigator |
SONODA Tatsuhiko Kitakyushu National College of Technology, 物質化学工学科, 准教授 (30403992)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | バイオチップ / 細胞内シグナル伝達 / ペプチドチップ / 酸化チタン / タンパク質リン酸化 / 網羅的解析 / プロテインキナーゼ |
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| Research Abstract |
A peptide array using a titanium oxide plate for detection of protein kinase activity has been investigated. First, the titanium oxide plate was prepared by sol-gel method. The plate has relatively flat surface and high hydrophilicity rate of photo-induced surface wettability conversion by UV irradiation. Furthermore, we succeeded that the surface wettability was controlled reversibly using hypobaric drying and UV irradiation. Next, complex with poly(acrylic)acid and protein kinase A (PKA) substrate peptide (PAA/PEP) was synthesized. We confirmed that PAA/PEP was phosphorylated by PKA, by coupled enzyme assay. Finally, fluorescenced poly(acrylic)acid (F-PAA) was immobilized on the titanium oxide plate, and the plate was sonicated in water. After washing, F-PAA still remained on the plate. It suggested that a bonding is formed between F-PAA and titanium oxide plate. From above results, we would establish the basis technique to develop the peptide array using the titanium oxide plate.
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Report
(3 results)
Research Products
(8 results)
