| Project/Area Number |
21710122
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
OKAMOTO Kenji The Institute of Physical and Chemical Research, 佐甲細胞情報研究室, 研究員 (40402763)
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| Research Collaborator |
HIBINO Kayo 独立行政法人理化学研究所, 佐甲細胞情報研究室, 研究員 (40435673)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | EGFR / 単一分子計測 / FRET / 統計的データ解析 / 高時間分解計測 / 分子構造変化ダイナミクス |
|---|
| Research Abstract |
Single molecule FRET measurement on conformational dynamics of the EGFR molecule, which is related to signal transduction on the cell membrane, has been developed. Conditions for preparation and fluorescence labeling of EGFR were optimized and FRET signals were successfully observed while the dynamics measurements could have not been completed since it had been taken longer time to optimize EGFR expression conditions than initial expectation. A new method of experimental data analysis based on the variational Bayes was also developed, which improved the accuracy and the time resolution.
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