Intracellular manipulation of proteins in neurons with resonance laser beam

• Project/Area Number: 21710123
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: HOSOKAWA Chie 独立行政法人産業技術総合研究所, 健康工学研究部門, 研究員 (60435766)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 光ピンセット / ナノバイオ / 脳 / 神経 / 生物物理 / 脳・神経

Research Abstract

Biological systems form complex networks related with functional molecules and are dynamically affected by spatio-temporal regulations. For aiming artificial control of the systems, we proposed and demonstrated intracellular manipulation of neural cell adhesion molecules(NCAMs) on living neuronal cells using resonance and nonresonance laser beams. The intracellular motion of NCAMs labeled with quantum dots was evaluated by fluorescence correlation spectroscopy.

Research Products

• [Journal Article] Optical trapping of synaptic vesicles in neurons (2011)
  • Author(s): Chie Hosokawa, Suguru N. Kudoh, Ai Kiyohara, and Takahisa Taguchi
  • Journal Title: Applied Physics Letters Volume: Vol.98 Issue: 16
  • DOI: 10.1063/1.3579191
  • Peer Reviewed
• [Journal Article] Vitroid-the robot system with an interface between a living neuronal network and outer world (2011)
  • Author(s): Suguru N. Kudoh, Minori Tokuda, Ai Kiyohara, Chie Hosokawa, Takahisa Taguchi, and Isao Hayashi
  • Journal Title: International Journal of Mechatronics and Manufacturing Systems Volume: Vol.4, No.2 Issue: 2 Pages: 135-149
  • DOI: 10.1504/ijmms.2011.039264
  • Peer Reviewed
• [Journal Article] Micro-channel fabrication by femtosecond laser to arrange neuronal cells on multi-electrode arrays (2010)
  • Author(s): Chie Hosokawa, Suguru N. Kudoh, Mariko Suzuki, Ai Kiyohara, Yoichiroh Hosokawa, Kazunori Okano, Hiroshi Masuhara, and Takahisa Taguchi
  • Journal Title: Applied Physics A Volume: Vol.101 Issue: 2 Pages: 423-428
  • DOI: 10.1007/s00339-010-5836-4
  • Peer Reviewed
• [Presentation] Laser-induced perturbation of single neuron in a neuronal network (2012)
  • Author(s): Chie Hosokawa, Yusuke Ueda, Yasutaka Sakamoto, Suguru N. Kudoh, and Takahisa Taguchi
  • Organizer: The 5th International Symposium on Nanomedicine(ISNM2012)
  • Place of Presentation: 名古屋大学
  • Year and Date: 2012-03-16
• [Presentation] Laser-Induced Perturbation of Single Neuron in a Neuronal Network (2012)
  • Author(s): C.Hosokawa, Y.Ueda, Y.Sakamoto, S.N.Kudoh, T.
  • Organizer: 5th International Symposium on Nanomedicine (ISNM2012)
  • Place of Presentation: 名古屋大学(愛知県)(招待講演)
  • Year and Date: 2012-03-16
• [Presentation] レーザー光摂動を用いた神経細胞ネットワーク操作過程の蛍光解析 (2012)
  • Author(s): 細川千絵、植田悠介、坂本泰隆、大西映里子、工藤卓、田口隆久
  • Organizer: 第24回バイオエンジニアリング講演会
  • Place of Presentation: 大阪大学
  • Year and Date: 2012-01-08
• [Presentation] レーザー光摂動を用いた神経細胞ネットワーク操作過程の蛍光解析 (2012)
  • Author(s): 細川千絵、植田悠介、坂本泰隆、大西映里子、工藤卓、田口
  • Organizer: 第24回バイオエンジニアリング講演会
  • Place of Presentation: 大阪大学(大阪府)(依頼講演)
  • Year and Date: 2012-01-08
• [Presentation] Optical trapping and assembling dynamics of synaptic vesicles in neurons revealed by fluorescence correlation spectroscopy, Neuroscience 2011 (2011)
  • Author(s): Chie Hosokawa, Yusuke Ueda, Suguru N. Kudoh, and Takahisa Taguchi
  • Organizer: The 41st annual meeting of SFN
  • Place of Presentation: Washington Convention Center, Washington DC, USA
  • Year and Date: 2011-11-13
• [Presentation] Optical trapping and assembling dynamics of synaptic vesicles in neurons revealed by fluorescence correlation spectroscopy (2011)
  • Author(s): C.Hosokawa, Y.Ueda, S.N.Kudoh, T.Taguchi
  • Organizer: Neuroscience 2011, The 41st annual meeting of SFN
  • Place of Presentation: Washington Convention Center, Washington DC, USA
  • Year and Date: 2011-11-13
• [Presentation] Optical perturbation of neuronal network by a focused laser beam (2011)
  • Author(s): 細川千絵、植田悠介、坂本泰隆、鈴木真理子、工藤卓、田口隆久
  • Organizer: 第26回生体・生理工学シンポジウム
  • Place of Presentation: 立命館大学
  • Year and Date: 2011-09-22
• [Presentation] Optical Perturbation of Neuronal Network by a Focused Laser Beam (2011)
  • Author(s): 細川千絵、植田悠介、坂本泰隆、鈴木真理子、工藤卓、田口
  • Organizer: 第26回生体・生理工学シンポジウム
  • Place of Presentation: 立命館大学(滋賀県)(依頼講演)
  • Year and Date: 2011-09-22
• [Presentation] Optical Perturbation of Neuronal Network by a Focused Laser Beam (2011)
  • Author(s): 細川千絵、植田悠介、坂本泰隆、鈴木真理子、工藤卓、田口隆久
  • Organizer: 第26回生体・生理工学シンポジウム
  • Place of Presentation: 立命館大学(滋賀県)
  • Year and Date: 2011-09-22
• [Presentation] 集光フェムト秒レーザーによる海馬神経細胞の光刺激 (2011)
  • Author(s): 坂本泰隆、細川千絵、工藤卓、田口隆久
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-09-15
• [Presentation] 光ピンセットによる神経細胞内シナプス小胞群の集合操作の蛍光解析 (2011)
  • Author(s): 植田悠介、細川千絵、工藤卓、田口隆久
  • Organizer: 第72回応用物理学会学術講演会
  • Place of Presentation: 山形大学
  • Year and Date: 2011-09-01
• [Presentation] 光ピンセットによる神経細胞内シナプス小胞群の集合操作の蛍光解析 (2011)
  • Author(s): 植田悠介、細川千絵、工藤卓、田口隆久
  • Organizer: 第72回応用物理学会学術講演会
  • Place of Presentation: 山形大学(山形県)
  • Year and Date: 2011-09-01
• [Presentation] 神経細胞内シナプス小胞群の光捕捉ダイナミクス (2011)
  • Author(s): 細川千絵、植田悠介、工藤卓、田口隆久
  • Organizer: ナノ学会第9回大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2011-06-02
• [Presentation] 神経細胞内シナプス小胞群の光捕捉ダイナミクス (2011)
  • Author(s): 細川千絵、植田悠介、工藤卓、田口隆久
  • Organizer: ナノ学会第9回大会
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2011-06-02
• [Presentation] 光捕捉された神経細胞内シナプス小胞群の蛍光相関分光解析 (2011)
  • Author(s): 細川千絵、植田悠介、工藤卓、大西映里子、鈴木真理子、田口隆久
  • Organizer: 第58回応用物理学関係連合講演会
  • Place of Presentation: 神奈川工科大学
  • Year and Date: 2011-03-25
• [Presentation] 光ピンセットを用いた培養神経細胞シナプス操作過程の蛍光解析 (2010)
  • Author(s): 細川千絵、大西映里子、工藤卓、田口隆久
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2010-09-03
• [Presentation] 集光レーザービームによる培養神経回路網の操作技術の開発 (2010)
  • Author(s): 細川千絵
  • Organizer: バイオメクフォーラム21第56回研究会
  • Place of Presentation: 大阪大学
  • Year and Date: 2010-04-17
• [Book] 医療応用バイオロボティクスへの発展 (2010)
  • Author(s): 工藤卓、細川千絵
• [Remarks]
  • URL: http://unit.aist.go.jp/gruop/birg/