Analyses of mammalian ribonecleoproteome dynamics via modified SELEX procedure.

Research Project

Project/Area Number	21710191
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	基礎ゲノム科学
Research Institution	The University of Tokyo
Principal Investigator	OHUCHI Shoji The University of Tokyo, 医科学研究所, 助教 (20422412)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords	ゲノム調節 / ncRNA / 機能性RNA / RNPome / ゲノム動態 / SELEX / アプタマー / リボヌクレオプロテオーム / 転写後発現調節
Research Abstract	In order to identify natural RNA elements which interact with intracellular proteins, I performed the modified SELEX procedure coupled with in silico screening employing whole human transcriptome library, and found the interactions between a transcription elongation regulator protein, HEXIM1, and several mRNAs. The result indicates the presence of a novel class of post-transcriptional gene regulatory mechanism mediated via RNA-protein interactions.