Analysis of transcriptional regulation by Cohesin

• Project/Area Number: 21710192
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 基礎ゲノム科学
• Research Institution: The University of Tokyo (2010); Tokyo Institute of Technology (2009)
• Principal Investigator: BANDO Masashige The University of Tokyo, 分子細胞生物学研究所, 助教 (90360627)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: ゲノム構造 / コヒーシン / アセチル化 / HDAC8 / SMC3 / クロマチン免疫沈降 / コルネリア・デ・ランゲ症候群 / ChIP-seq / アセチル化修飾 / ChIP-seuence法 / マウス胎児 / 転写制御

Research Abstract

I have developed a system to deplete SMC3, one of essential cohesin subunits, specifically in the neuron cells of mice. The neuron could not survive and cell death was induced. I am analyzing gene expression profile and chromatin high order structure at each stage of differentiation. Meanwhile I have identified HDAC8 as a specific deaceylase for SMC3 and causative gene for CdLS. These findings suggest that acetylation status of cohesin is also important for transcriptional regulation. I am now trying to analyze biochemical property of acetylated Smc3 and its genomic localization to understand the mechanism behind regulation of transcription by acetylation.

Research Products

• [Journal Article] Sororin mediates sister chromatid cohesion by antagonizing Wapl (2010)
  • Author(s): Nishiyama T, Ladurner R, Schmitz J, Kreidl E, Schleiffer A, Bhaskara V, Bando M, Shirahige K, Hyman AA, Mechtler K, Peters JM
  • Journal Title: Cell 143 Pages: 737-749
  • Peer Reviewed
• [Journal Article] Genome-wide DNA methylation analysis in cohesin mutant human cell lines (2010)
  • Author(s): Liu J, Zhang Z, Bando M, Itoh T, Deardorff MA, Li JR, Clark D, Kaur M, Tatsuro K, Kline AD, Chang C, Vega H, Jackson LG, Spinner NB, Shirahige K, Krantz ID
  • Journal Title: Nucleic Acids Res. 38 Pages: 5657-5671
  • Peer Reviewed
• [Journal Article] Sororin mediates sister chromatid cohesion by antagonizing Wap1 (2010)
  • Author(s): Nishiyama T, Ladurner R, Schmitz J, Kreidl E, Schleiffer A, Bhaskara V, Bando M, Shirahige K, Hyman AA, Mechtler K, Peters JM
  • Journal Title: Cell Volume: 143 Pages: 737-749
  • Peer Reviewed
• [Journal Article] Genome-wide DNA methylation analysis in cohesin mutant human cell lines (2010)
  • Author(s): Liu J, Zhang Z, Bando M, Itoh T, Deardorff MA, Li JR, Clark D, Kaur M, Tatsuro K, Kline AD, Chang C, Vega H, Jackson LG, Spinner NB, Shirahige K, Krantz ID
  • Journal Title: Nucleic Acids Res. Volume: 38 Pages: 5657-5671
  • Peer Reviewed
• [Journal Article] Csm3, Tof1, and Mrc1 form a heterotrimeric mediator complex that associates with DNA replication forks. (2009)
  • Author(s): Bando M, Katou Y, Komata M, Tanaka H, Itoh T, Sutani T, Shirahige K
  • Journal Title: J.biol.chem 284 Pages: 34355-34365
  • Peer Reviewed
• [Journal Article] Ctf4 coordinates the progression of helicase and DNA polymerase α (2009)
  • Author(s): Tanaka H, Katou Y, Yagura M, Saitoh K, Itoh T, Araki H, Bando M, Shirahige K
  • Journal Title: Genes to Cell 14 Pages: 807-820
  • Peer Reviewed
• [Journal Article] Transcriptional Dysregulation in NIPBL and Cohesin Mutant Human Cells (2009)
  • Author(s): Liu J, Zhang Z, Bando M, Itoh T, Deardorff MA, Clark D, Kaur M, Tandy S, Kondoh T, Rappaport E, Spinner NB, Vega H, Jackson LG, Shirahige K, Krantz ID
  • Journal Title: Pros Biology 7
  • Peer Reviewed
• [Journal Article] Csm3, Tof1, and Mrc1 form a heterotrimeric mediator complex that associates with DNA replication forks (2009)
  • Author(s): Bando M, Katou Y, Komata M, Tanaka H, Itoh T, Sutani T, Shirahige K
  • Journal Title: J.biol.chem 284 Pages: 34355-34365
  • Peer Reviewed
• [Presentation] SMC3脱アセチル化酵素HDAC8の生理的意義の解明 (2010)
  • Author(s): 坂東優篤、斉藤勝也、高垣謙太郎、野崎直人、広田亨、伊藤武彦、白髭克彦
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07