Analysis of transcriptional regulation by Cohesin
Project/Area Number |
21710192
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
基礎ゲノム科学
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Research Institution | The University of Tokyo (2010) Tokyo Institute of Technology (2009) |
Principal Investigator |
BANDO Masashige The University of Tokyo, 分子細胞生物学研究所, 助教 (90360627)
|
Project Period (FY) |
2009 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | ゲノム構造 / コヒーシン / アセチル化 / HDAC8 / SMC3 / クロマチン免疫沈降 / コルネリア・デ・ランゲ症候群 / ChIP-seq / アセチル化修飾 / ChIP-seuence法 / マウス胎児 / 転写制御 |
Research Abstract |
I have developed a system to deplete SMC3, one of essential cohesin subunits, specifically in the neuron cells of mice. The neuron could not survive and cell death was induced. I am analyzing gene expression profile and chromatin high order structure at each stage of differentiation. Meanwhile I have identified HDAC8 as a specific deaceylase for SMC3 and causative gene for CdLS. These findings suggest that acetylation status of cohesin is also important for transcriptional regulation. I am now trying to analyze biochemical property of acetylated Smc3 and its genomic localization to understand the mechanism behind regulation of transcription by acetylation.
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Report
(3 results)
Research Products
(9 results)
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[Journal Article] Sororin mediates sister chromatid cohesion by antagonizing Wapl2010
Author(s)
Nishiyama T, Ladurner R, Schmitz J, Kreidl E, Schleiffer A, Bhaskara V, Bando M, Shirahige K, Hyman AA, Mechtler K, Peters JM
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Genome-wide DNA methylation analysis in cohesin mutant human cell lines2010
Author(s)
Liu J, Zhang Z, Bando M, Itoh T, Deardorff MA, Li JR, Clark D, Kaur M, Tatsuro K, Kline AD, Chang C, Vega H, Jackson LG, Spinner NB, Shirahige K, Krantz ID
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Journal Title
Nucleic Acids Res. 38
Pages: 5657-5671
Related Report
Peer Reviewed
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[Journal Article] Sororin mediates sister chromatid cohesion by antagonizing Wap12010
Author(s)
Nishiyama T, Ladurner R, Schmitz J, Kreidl E, Schleiffer A, Bhaskara V, Bando M, Shirahige K, Hyman AA, Mechtler K, Peters JM
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Journal Title
Cell
Volume: 143
Pages: 737-749
Related Report
Peer Reviewed
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[Journal Article] Genome-wide DNA methylation analysis in cohesin mutant human cell lines2010
Author(s)
Liu J, Zhang Z, Bando M, Itoh T, Deardorff MA, Li JR, Clark D, Kaur M, Tatsuro K, Kline AD, Chang C, Vega H, Jackson LG, Spinner NB, Shirahige K, Krantz ID
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Journal Title
Nucleic Acids Res.
Volume: 38
Pages: 5657-5671
Related Report
Peer Reviewed
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[Journal Article] Transcriptional Dysregulation in NIPBL and Cohesin Mutant Human Cells2009
Author(s)
Liu J, Zhang Z, Bando M, Itoh T, Deardorff MA, Clark D, Kaur M, Tandy S, Kondoh T, Rappaport E, Spinner NB, Vega H, Jackson LG, Shirahige K, Krantz ID
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Journal Title
Related Report
Peer Reviewed
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