| Project/Area Number |
21710196
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
基礎ゲノム科学
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
SHIBA Hiroshi Nara Institute of Science and Technology, バイオサイエンス研究科, 助教 (20294283)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | DNAメチル化 / アンチセンス転写産物 / メチローム / ゲノムタイリングアレイ / aberrant RNA / 次世代シークエンサー |
|---|
| Research Abstract |
DNA methylation within the gene body is thought to impede transcriptional elongation, but the causal role of genic DNA methylation remains essentially unknown. In this research, I have generated DNA methylation profile (methylome) as well as sense and antisense transcription profiles (transcriptome), at 35-base pair resolution, of the wild-type and the loss-of DNA methyltransferase mutant of Arabidopsis. Combinational analyses of the methylomes and the transcriptomes have indicated that some sense/antisense transcripts are associated with body methylation. I also examined correlation between body methylation and small RNAs.
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