Functional analyses of antisense transcripts in the epigenetic regulation of gene expression
| Project/Area Number |
21710196
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
基礎ゲノム科学
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
SHIBA Hiroshi Nara Institute of Science and Technology, バイオサイエンス研究科, 助教 (20294283)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | DNAメチル化 / アンチセンス転写産物 / メチローム / ゲノムタイリングアレイ / aberrant RNA / 次世代シークエンサー |
|---|
| Research Abstract |
DNA methylation within the gene body is thought to impede transcriptional elongation, but the causal role of genic DNA methylation remains essentially unknown. In this research, I have generated DNA methylation profile (methylome) as well as sense and antisense transcription profiles (transcriptome), at 35-base pair resolution, of the wild-type and the loss-of DNA methyltransferase mutant of Arabidopsis. Combinational analyses of the methylomes and the transcriptomes have indicated that some sense/antisense transcripts are associated with body methylation. I also examined correlation between body methylation and small RNAs.
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Report
(3 results)
Research Products
(30 results)
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[Presentation] アブラナ科植物の自家不和合性でみられるエピジェネティックな遺伝子発現制御2010
Author(s)
Hiroshi Shiba, Taketo Kayabe, Kentaro Yanol, Junshi Yazaki, Huaming Chen, Yutaka Suzuki, Masahiro Fujita, Nori Kurata, Akira Isogai, Joseph R.Ecker, Seiji Takayama.
Organizer
日本遺伝学会
Place of Presentation
札幌
Year and Date
2010-09-20
Related Report
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[Presentation] Genome-wide analyses of allele-specific expression and DNA methylation in Arabidopsis thaliana2010
Author(s)
柴博史, 栢部健人, Kentaro Yanol, 矢崎潤史, Huaming Chen, 鈴木剛, 藤田雅丈, 倉田のり, 磯貝彰, Joseph R.Ecker, 高山誠司
Organizer
21st International Conference on Arabidopsis Research
Place of Presentation
Yokohama
Year and Date
2010-06-08
Related Report
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[Presentation]2010
Author(s)
柴博史, 栢部健人, 樽谷芳明, 藤田雅丈, 倉田のり, 矢崎潤史, Joseph R.Ecker, 磯貝彰, 高山誠司
Organizer
第51回日本植物生理学会
Place of Presentation
熊本
Year and Date
2010-03-18
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