| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Research Abstract |
We focused on SP120, one of the nuclear matrix-associated proteins to develop MARcode, a prediction program for matrix attachment regions (MAR). It is suggested that SP120 is involved in the neuronal differentiation through its binding to DNA topoisomerase (topo) IIβ. We showed that SP120 specifically bound to AT-rich topoIIβ action sites. We performed a domain analysis for MAR-binding activity of SP120 and found that RG-domain had the activity. We showed the evidence that SP120 directly bound to AT patches (short stretches of consecutive A and T bases). We performed a genome-wide analysis to identify in vivo SP120 binding sites. We will further analyze the identified regions to develop the MARcode program.
|
