Analysis of Transcriptional Regulation of Genome by Pathogenicity Transcription Factor LeuO.

• Project/Area Number: 21710198
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 基礎ゲノム科学
• Research Institution: Hosei University
• Principal Investigator: SHIMADA Tomohiro Hosei University, マイクロ・ナノテクノロジー研究センター, 研究員 (10535230)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: ゲノム発現制御 / 転写 / 病原性細菌 / 転写因子LeuO / Genomic SELEX / 薬剤耐性 / サルファ剤耐性遺伝子 / SELEX-chip / バイオフィルム / 転写因子Cra / 転写因子CRP / 大腸菌

Research Abstract

To get insights into the regulatory role(s) of LeuO, attempts were made to identify the whole set of regulation targets using Genomic SELEX system. A total of 140 LeuO-binding sites were identified on the E.coli genome, 125 of which were newly identified. Taken together with our previous finding of the involvement of LeuO in mutli-drug efflux, it was revealed that LeuO regulates a number of genes for biofilm formation including the genes for fimbriae formation.

Research Products

• [Journal Article] Novel Members of the cAMP Receptor Protein (CRP) Regulon for Transport and Metabolism of Carbon Sources. (2011)
  • Author(s): Shimada, T., Yamamoto, K., Fujita, N., Ishihama, A.
  • Journal Title: PLoS ONE. in press
  • Peer Reviewed
• [Journal Article] Novel Members of the Cra Regulon Involved in Carbon Metabolism in Escherichia coli. (2011)
  • Author(s): Shimada, T., Yamamoto, K., Ishihama, A.
  • Journal Title: Journal of Bacteriology. 193 Pages: 649-59
  • URL: http://jb.asm.org/cgi/content/full/193/3/649?view=long&pmid=21115656
  • Peer Reviewed
• [Journal Article] Novel Members of the Cra Regulon Involved in Carbon Metabolism in Escherichia coli (2011)
  • Author(s): Tomohiro Shimada
  • Journal Title: Journal of Bacteriology Volume: 193 Pages: 649-659
  • Peer Reviewed
• [Journal Article] Novel Roles of cAMP Receptor Protein (CRP) in Regulation of Transport and Metabolism of Carbon Sources (2011)
  • Author(s): Tomohiro Shimada
  • Journal Title: PLoS ONE Volume: (掲載確定)
  • Peer Reviewed
• [Journal Article] Involvement of the leucine response transcription factor LeuO in regulation of the genes for sulfa drug efflux. (2009)
  • Author(s): Shimada, T., Yamamoto, K., Ishihama, A.
  • Journal Title: Journal of Bacteriology. 191 Pages: 4562-71
  • URL: http://jb.asm.org/cgi/content/full/191/14/4562?view=long&pmid=19429622
  • Peer Reviewed
• [Journal Article] Involvement of the leucine response transcription factor LeuO in regulation of the genes for sulfa drug efflux. (2009)
  • Author(s): Tomohiro Shimada
  • Journal Title: Journal of Bacteriology. 191 Pages: 4562-71
  • Peer Reviewed
• [Presentation] ふたつの転写因子CRPとCraによる大腸菌炭素源代謝遺伝子群の転写制御の全体像 (2011)
  • Author(s): 島田友裕
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都女子大学(京都)
  • Year and Date: 2011-03-26
• [Presentation] ふたつの転写因子CRPとCraによる大腸菌炭素源代謝遺伝子群の転写制御の全体像 (2011)
  • Author(s): 島田友裕, 藤田信之, 山本兼由, 石浜明
  • Organizer: 2011年度日本農芸化学会大会
  • Place of Presentation: 京都女子大学・京都
• [Presentation] 大腸菌炭素源代謝制御のふたつの転写因子CRPとCraによるゲノム転写制御の全体像:Genomic SELEX法を用いた解析 (2010)
  • Author(s): 島田友裕
  • Organizer: 生化・分子生物学合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫)
  • Year and Date: 2010-12-07
• [Presentation] Genome-wide Genomic-SELEX Search of Regulation Targets by Transcription Factors. (2010)
  • Author(s): Shimada, T., et al.
  • Organizer: Jacques Monod Commemorative Minisymposium.
  • Place of Presentation: Paris. France.
  • Year and Date: 2010-05-31
• [Presentation] Genome-wide Genomic-SELEX Search of Regulation Targets by Transcription Factors (2010)
  • Author(s): Tomohiro Shimada
  • Organizer: Jacques Monod Commemorative Mini symposium
  • Place of Presentation: パリ(フランス)
  • Year and Date: 2010-05-31
• [Presentation] 大腸菌転写因子CRPのゲノム上結合部位の全体像:Genomic SELEX法を用いた解析 (2010)
  • Author(s): 島田友裕
  • Organizer: 第4回日本ゲノム微生物学会年会
  • Place of Presentation: 九州大学(福岡)
  • Year and Date: 2010-03-08
• [Presentation] 大腸菌炭素源代謝制御のふたつの転写因子CRPとCraによるゲノム転写制御の全体像: Genomic SELEX法を用いた解析 (2010)
  • Author(s): 島田友裕, 藤田信之, 山本兼由, 石浜明
  • Organizer: 生化・分子生物学会合同大会
  • Place of Presentation: 神戸ポートアイランド・兵庫
• [Presentation] 大腸菌転写因子CRPのゲノム上結合部位の全体像: Genomic SELEX法を用いた解析 (2010)
  • Author(s): 島田友裕, 藤田信之, 山本兼由, 石浜明
  • Organizer: 第四回日本ゲノム微生物学会年会
  • Place of Presentation: 九州大学・福岡県
• [Presentation] Involvement of the Leucine Response Transcription Factor LeuO in Regulation of the Genes for Sulfa Drug Efflux (2009)
  • Author(s): Tomohiro Shimada
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2009-12-09
• [Presentation] Genomic SELEX for the Genome-wide Search of Regulation Targets by Transcription Factors : SELEX-clos and SELEX-chip Procedures (2009)
  • Author(s): Tomohiro Shimada
  • Organizer: 2009 International Symposium on Micro-NanoMechatronics and Human Science
  • Place of Presentation: 名古屋大学(愛知)
  • Year and Date: 2009-11-09
• [Presentation] Novel technologies for the genome-wide search of regulation targets by transcription factors : genomic SELEX and SELEX-chip. Micro- and Nano-Mechatronics and Human Science (2009)
  • Author(s): Shimada, T., et al.
  • Organizer: 2009 IEEE International Symposium on IEEE Robotics and Automation Society, USA.
  • Place of Presentation: Nagoya. Japan.
• [Presentation] Growth phase-dependent regulation of csgD, the master regulator of biofilm formation : Interplay between multiple transcription factors. (2009)
  • Author(s): Ishihama, A., Ogasawara, H., Shimada, T., Teramoto, J., Kori,A., Yamada, K., Yamamoto, K.
  • Organizer: 3^<rd> Internatl. Conf. Environmental, Industrial and Applied Microbiology (BioMicroWorld 2009)
  • Place of Presentation: Lisbon, Portugal.
• [Presentation] Comprehensive analysis for the recognition sequences of DNA-binding transcription factors within the E.coli genome using the newly developed promoter chip. (2009)
  • Author(s): Yamamoto, K., Shimada, T., Ishihama, A.
  • Organizer: 21^<st> RNA Polymerase Workshop
  • Place of Presentation: Bristol, UK
• [Presentation] Involvement of the Leucine Response Transcription Factor LeuO in Regulation of the Genes for Sulfa Drug Efflux (2009)
  • Author(s): Tomohiro Shimada, Kaneyoshi Yamamoto, Akira Ishihama
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜・神奈川
• [Presentation] Genomic SELEXを用いた大腸菌転写因子LeuOの転写制御機構解析 (2009)
  • Author(s): 島田友裕, 山本兼由, 石浜明
  • Organizer: 第6回21世紀大腸菌研究会
  • Place of Presentation: KKRホテル熱海・静岡
• [Book] Genomic SELEX for the genome-wide search of regulation targets by transcription factors : SELEX-clos and SELEX-chip procedures. In: Micro- and Nano-Mechatronics and Human Science (Fukuda, T. eds.) (2009)
  • Author(s): Shimada, T., et al.