A systematic search for potential substrates of deubiquitinase with quantitative proteomics

• Project/Area Number: 21710200
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied genomics
• Research Institution: The University of Tokyo
• Principal Investigator: OTA Kazuhisa The University of Tokyo, 大学院・理学系研究科, 助教 (00322727)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 翻訳後修飾 / ユビキチン / 出芽酵母 / プロテオミクス / 質量分析

Research Abstract

Combining our originally poly-ubiquitinated protein enrichment method and high throughput LC-MS/MS analysis, we tried to identify specific substrates for all USP type deubiquitinases coded in the genome of Saccharomyces cerevisiae. Finally, we identified 94 potential substrate-enzyme relationships and, for some of these, made validation with molecular biological methods.

Research Products

• [Journal Article] A yeast one-hybrid system to screen for methylated DNA-binding proteins. (2010)
  • Author(s): Feng SY., Ota K., Ito T.
  • Journal Title: Nucleic Acids Res.
  • Peer Reviewed
• [Journal Article] A yeast one-hybrid system to screen for methylated DNA-binding proteins (2010)
  • Author(s): Feng SY, Ota K, Ito T.
  • Journal Title: Nucleic Acids Research Volume: 38
  • Peer Reviewed
• [Journal Article] Remodeling of the SCF complex-mediated ubiquitination system by compositional alteration of incorporated F-box proteins (2010)
  • Author(s): Kato, M., Kito, K., Ota, K.Ito, T.
  • Journal Title: Proteomics 10 Pages: 115-123
  • Peer Reviewed
• [Journal Article] Remodeling of the SCF Complex-mediated Ubiquitination System by Compositional (2009)
  • Author(s): Kato M., Kito K., Ota K., Ito T.
  • Journal Title: Alteration of Incorporated F-box Proteins Proteomics
  • Peer Reviewed
• [Journal Article] Circular permutation of ligand-binding module improves dynamic range of genetically encoded FRET-based nanosensor. (2009)
  • Author(s): Okada S., Ota K., Ito T.
  • Journal Title: Protein Sci.
  • Peer Reviewed
• [Presentation] RSP5機能のユビキチン化による制御機構 (2010)
  • Author(s): 太田一寿、紀藤圭治、伊藤隆司
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] ミトコンドリア外膜蛋白質MDM34のユビキチン化機構 (2009)
  • Author(s): 太田一寿・岡田悟・伊藤隆司
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] USP型脱ユビキチン化酵素の網羅的基質探索 (2009)
  • Author(s): 廣田剛志・太田一寿・紀藤圭治・伊藤隆司
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-10
• [Remarks] ホームページ
  • URL: http://itolab.k.u-tokyo.ac.jp
• [Remarks]
  • URL: http://itolab.k.u-tokyo.ac.jp
• [Remarks]
  • URL: http://itolab2.cb.k.u-tokyo.ac.jp/