Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Research Abstract |
Combining our originally poly-ubiquitinated protein enrichment method and high throughput LC-MS/MS analysis, we tried to identify specific substrates for all USP type deubiquitinases coded in the genome of Saccharomyces cerevisiae. Finally, we identified 94 potential substrate-enzyme relationships and, for some of these, made validation with molecular biological methods.
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